Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin.

abstract：:The impact of overproduction of a heterologous protein on the metabolic system of host Lactococcus lactis was investigated. The protein expression profiles of L. lactis IL1403 containing two near-identical plasmids that expressed high- and low-level of the green fluorescent protein (GFP) were examined via shotgun prot...

journal_title：Journal of microbiology and biotechnology

authors： Kim M,Jin Y,An HJ,Kim J

更新日期：2017-07-28 00:00:00

abstract：:Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59-66% as analyzed by TLC and HPL...

journal_title：Journal of microbiology and biotechnology

authors： Ko JA,Ryu YB,Park JY,Kim CY,Kim JS,Nam SH,Lee WS,Kim YM

更新日期：2016-03-01 00:00:00

abstract：:To increase the availability of microalgae as producers of valuable compounds, it is necessary to develop novel systems for gene expression regulation. Among the diverse expression systems available in microalgae, none are designed to induce expression by low temperature. In this study, we explored a cold-inducible sy...

journal_title：Journal of microbiology and biotechnology

authors： Kim M,Kim J,Kim S,Jin E

更新日期：2020-11-28 00:00:00

abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...

journal_title：Journal of microbiology and biotechnology

authors： Kim HJ,Jung WK,Lee HW,Yoo W,Kim TD,Kim H

更新日期：2015-12-28 00:00:00

abstract：:In an ongoing investigation of compounds from natural products that exhibit anti-aging properties, hydroxyhibiscone A (1), a new furanosesquiterpenoid, together with hibiscone D (2), was isolated from the root bark of Hibiscus syriacus. Utilizing UV, IR, NMR, and MS spectroscopic analyses, these chemical structures we...

journal_title：Journal of microbiology and biotechnology

authors： Ryoo IJ,Yun BS,Lee IK,Kim YH,Lee IS,Ahn JS,Bae K,Yoo ID

更新日期：2010-08-01 00:00:00

abstract：:An electron paramagnetic resonance (EPR) signal characteristic of the 5,5'-dimethyl-1-pyrroline-N-oxide (DMPO)-OH spin adduct, which is formed from the reaction of DMPO with superoxide radicals generated by xanthine oxidase-mediated reaction, was significantly reduced by the cadaverine or Escherichia coli Mn-containin...

journal_title：Journal of microbiology and biotechnology

authors： Kang IH,Kim JS,Kim EJ,Lee JK

更新日期：2007-01-01 00:00:00

abstract：:The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen ty...

journal_title：Journal of microbiology and biotechnology

authors： Gil CS,Chang MS,Eom SH

更新日期：2017-08-28 00:00:00

abstract：:We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detectio...

journal_title：Journal of microbiology and biotechnology

authors： Ji GY,Song HG,Son BR,Hong SB,Kim JW,Shin KS

更新日期：2014-03-28 00:00:00

abstract：:A gene (acas) designated as alpha-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of alpha-amylase. In fact, the recombinant enzyme exhibited typical ASase activi...

journal_title：Journal of microbiology and biotechnology

authors： Seo DH,Jung JH,Choi HC,Cho HK,Kim HH,Ha SJ,Yoo SH,Cha J,Park CS

更新日期：2012-09-01 00:00:00

abstract：:Ahead of Print article withdrawn by publisher. ...

journal_title：Journal of microbiology and biotechnology

authors： Farajzadah Sheikh A,Aslani S,Hosseini SA,Amin M,Goodarzi H,Jomehzadeh N,Ranjbar R,Moradzadeh M,Azarpira S,Moradi M,Hashemzadeh M

更新日期：2018-05-08 00:00:00

abstract：:Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, there was not much information on antibiotic resistance of Halomonas species. As an effort to find antibiotic resistance of Halomonas socia CKY01, which showed produ...

journal_title：Journal of microbiology and biotechnology

authors： Park YL,Choi TR,Kim HJ,Song HS,Lee HS,Park SL,Lee SM,Kim SH,Park S,Bhatia SK,Gurav R,Sung C,Seo SO,Yang YH

更新日期：2020-11-04 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are biodegradable plastics produced by bacteria, but their use in diverse applications is prohibited by high production costs. To reduce these costs, the conversion by Pseudomonas strains of P HAs from crude s ludge p alm oil ( SPO) a s an inexpensive renewable raw material was tested. Pse...

journal_title：Journal of microbiology and biotechnology

authors： Kang DK,Lee CR,Lee SH,Bae JH,Park YK,Rhee YH,Sung BH,Sohn JH

更新日期：2017-05-28 00:00:00

abstract：:The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-β2m-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hinder...

journal_title：Journal of microbiology and biotechnology

authors： Xu T,Li X,Wu Y,Shahzad KA,Wang W,Zhang L,Shen C

更新日期：2016-12-28 00:00:00

abstract：:Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and...

journal_title：Journal of microbiology and biotechnology

authors： Kim YO,Park IS,Nam BH,Kim DG,Jee YJ,Lee SJ,An CM

更新日期：2014-09-01 00:00:00

abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...

journal_title：Journal of microbiology and biotechnology

authors： Lee JK,Shin OS

更新日期：2020-12-31 00:00:00

abstract：:The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and fi...

journal_title：Journal of microbiology and biotechnology

authors： Kim HL,Shin E,Kim HM,Go H,Roh J,Bae J,Lee K

更新日期：2007-10-01 00:00:00

abstract：:Proteomic analysis led to identification of the proteins of Vibrio vulnificus that were induced upon exposure to INT-407 cells, and 7 of which belong to the functional categories such as amino acid transport/metabolism, nucleotide transport/metabolism, posttranslational modification/protein turnover/chaperones, and tr...

journal_title：Journal of microbiology and biotechnology

authors： Oh MH,Jeong HG,Choi SH

更新日期：2008-05-01 00:00:00

abstract：:Tuberculosis, which is caused by Mycobacterium tuberculosis (Mtb), is among the most pressing worldwide problems. Mtb uniquely interacts with innate immune cells through various pattern recognition receptors. These interactions initiate several inflammatory pathways that play essential roles in controlling Mtb pathoge...

journal_title：Journal of microbiology and biotechnology

authors： Kim JS,Kim YR,Yang CS

更新日期：2019-10-28 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:In this study, a method of heat adaptation was implemented in an attempt to increase the upper thermal threshold of two Streptococcus thermophilus found in South Korea and identified the alterations in membrane fatty acid composition to adaptive response to heat. In order to develop heat tolerant lactic acid bacteria,...

journal_title：Journal of microbiology and biotechnology

authors： Min B,Kim K,Li V,Cho S,Kim H

更新日期：2020-05-28 00:00:00

abstract：:Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells ...

journal_title：Journal of microbiology and biotechnology

authors： Kim SJ,Kim IW,Kwon YN,Yun EY,Hwang JS

更新日期：2012-02-01 00:00:00

abstract：:A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of ...

journal_title：Journal of microbiology and biotechnology

authors： Vyas P,Joshi R,Sharma KC,Rahi P,Gulati A,Gulati A

更新日期：2010-12-01 00:00:00

abstract：:Profiling the transcriptome changes involved in xylose metabolism by the fungus Trichoderma reesei allows for the identification of potential targets for ethanol production processing. In the present study, the transcriptome of T. reesei HJ-48 grown on xylose versus glucose was analyzed using nextgeneration sequencing...

journal_title：Journal of microbiology and biotechnology

authors： Huang J,Lin M,Liang S,Qin Q,Liao S,Lu B,Wang Q

更新日期：2020-10-28 00:00:00

abstract：:Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...

journal_title：Journal of microbiology and biotechnology

authors： Oh KH,Cho SH

更新日期：2014-03-28 00:00:00

abstract：:Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosy...

journal_title：Journal of microbiology and biotechnology

authors： Pranawidjaja S,Choi SI,Lay BW,Kim P

更新日期：2015-06-01 00:00:00

abstract：:Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identificat...

journal_title：Journal of microbiology and biotechnology

authors： Chen C,Zhong G,Ren L,Lu C,Li Z,Wu Y

更新日期：2015-10-01 00:00:00

abstract：:While searching for lactic acid bacteria that can restore aging-impaired immune responses, we isolated the Toll-like receptor (TLR) 2/NF-kappaB-activating strain Lactobacillus plantarum HY7712 from kimchi and investigated its immunomodulating effect in whole-body γ-irradiated mice. Exposure to HY7712 strongly activate...

journal_title：Journal of microbiology and biotechnology

authors： Lee H,Ahn YT,Park SH,Park DY,Jin YW,Kim CS,Sung SH,Huh CS,Kim DH

更新日期：2014-01-01 00:00:00

abstract：:Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobiliz...

journal_title：Journal of microbiology and biotechnology

authors： Yao J,Chen Q,Zhong G,Cao W,Yu A,Liu Y

更新日期：2014-01-01 00:00:00

abstract：:We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of ...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Hong J,Zhang P,Lu LF,Hwang JS,Park KC,Kim H

更新日期：2015-10-01 00:00:00

abstract：:Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms....

journal_title：Journal of microbiology and biotechnology

authors： Kim JE,Monmai C,Rod-In W,Jang AY,You SG,Lee SM,Park WJ

更新日期：2019-09-28 00:00:00