Latest Comprehensive Knowledge of the Crosstalk between TLR Signaling and Mycobacteria and the Antigens Driving the Process.

abstract：:The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-β2m-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hinder...

journal_title：Journal of microbiology and biotechnology

authors： Xu T,Li X,Wu Y,Shahzad KA,Wang W,Zhang L,Shen C

更新日期：2016-12-28 00:00:00

abstract：:Cryptomonads are unicellular, biflagellate algae. Generally, cryptomonad cells cannot be preserved well because of their fragile nature, and an improved methodology should be developed to identify cryptomonads from natural habitats. In this study, we tried using several cytological fixatives, including glutaraldehyde,...

journal_title：Journal of microbiology and biotechnology

authors： Xia S,Cheng Y,Zhu H,Liu G,Hu Z

更新日期：2013-03-01 00:00:00

abstract：:We previously reported that the CopA3 peptide (LLCIALRKK, D-form) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the D-form, has limited the development of CopA3 for therapeutic purposes. Here, we in...

journal_title：Journal of microbiology and biotechnology

authors： Yoon IN,Hong J,Zhang P,Hwang JS,Kim H

更新日期：2017-02-28 00:00:00

abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...

journal_title：Journal of microbiology and biotechnology

authors： Kim HJ,Jung WK,Lee HW,Yoo W,Kim TD,Kim H

更新日期：2015-12-28 00:00:00

abstract：:The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic wa...

journal_title：Journal of microbiology and biotechnology

authors： Ryszard CJ,Tomasz A,Kalinowska K,Skowronska A

更新日期：2009-09-01 00:00:00

abstract：:In this study, two soybean genotypes i.e. aluminum-tolerant Baxi 10 (BX10) and aluminum-sensitive Bendi 2 (BD2) were used as plant materials and the acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after ...

journal_title：Journal of microbiology and biotechnology

authors： Wen ZL,Yang MK,Fazal A,Liao YH,Cheng LR,Hua XM,Hu DQ,Shi JS,Yang RW,Lu GH,Qi JL,Hong Z,Qian QP,Yang YH

更新日期：2020-08-28 00:00:00

abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...

journal_title：Journal of microbiology and biotechnology

authors： Long D,Tang X,Cai K,Chen G,Shen C,Shi J,Chen L,Chen Y

更新日期：2013-08-01 00:00:00

abstract：:Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is hi...

journal_title：Journal of microbiology and biotechnology

authors： Choi IY,Lee JH,Kim HJ,Park MK

更新日期：2017-12-28 00:00:00

abstract：:Chitosan-based film-forming gel is regarded as a promising vehicle for topical delivery of antimicrobial agents to skin wounds, since it protects from microbial infection and the cationic polymer itself possesses antibacterial activity. In this study, possible synergistic interaction against common skin pathogens betw...

journal_title：Journal of microbiology and biotechnology

authors： Han SD,Sung HJ,Lee GH,Jun JH,Son M,Kang MJ

更新日期：2016-05-28 00:00:00

abstract：:Monoamine oxidase (MAO) is found in most cell types and catalyzes the oxidation of monoamines. Three anithiactins (A-C, modified 2-phenylthiazoles) isolated from Streptomyces sp. were tested for inhibitory activity of two isoforms, MAO-A and MAO-B. Anithiactin A was effective and selective for the inhibition of MAO-A,...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Jung WK,Kim HJ,Jeong YS,Nam SJ,Kang H,Kim H

更新日期：2015-09-01 00:00:00

abstract：:Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

journal_title：Journal of microbiology and biotechnology

authors： Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

更新日期：2013-04-01 00:00:00

abstract：UNLABELLED:We have previously shown that paraquat (PQ)-induced oxidative stress causes dramatic damage in various human cell lines. Naringenin (NG) is an active flavanone, which has been reported to have beneficial bioactivities, including antioxidative, anti-inflammatory, and antitumorigenic activities, with a relativ...

journal_title：Journal of microbiology and biotechnology

authors： Podder B,Song HY,Kim YS

更新日期：2014-05-01 00:00:00

abstract：:Beta-1,3-glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. beta-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of t...

journal_title：Journal of microbiology and biotechnology

authors： Shim JH,Sung KJ,Cho MC,Choi WA,Yang Y,Lim JS,Yoon DY

更新日期：2007-09-01 00:00:00

abstract：:The aim of this study was to analyze the chemical composition of 14 kinds of citrus oils and to test their biological activities. Citrus essential oils were obtained by steam distillation from immature fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)- flame ionization detectors (FID) an...

journal_title：Journal of microbiology and biotechnology

authors： Baik JS,Kim SS,Lee JA,Oh TH,Kim JY,Lee NH,Hyun CG

更新日期：2008-01-01 00:00:00

abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Qian H,Zhang WG

更新日期：2010-01-01 00:00:00

abstract：:This study compared the radioprotective effects of high-molecular-weight poly-gamma-glutamate (γ-PGA, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence int...

journal_title：Journal of microbiology and biotechnology

authors： Oh YJ,Kwak MS,Sung MH

更新日期：2018-04-28 00:00:00

abstract：:Staphylococcus aureus strains are important foodborne pathogens that produce various toxins. To evaluate the risk of the enterotoxins, four S. aureus strains from kinbap and two clinical samples were isolated and identified, and their expression of the enterotoxin genes were analyzed using a reverse transcription real...

journal_title：Journal of microbiology and biotechnology

authors： Lee YD,Moon BY,Park JH,Chang HI,Kim WJ

更新日期：2007-03-01 00:00:00

abstract：:Succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde (SSA) into succinic acid in the final step of γ-aminobutyric acid degradation. Here, we characterized Bacillus subtilis SSADH (BsSSADH) regarding its cofactor discrimination and substrate inhibition. BsSSADH showed similar val...

journal_title：Journal of microbiology and biotechnology

authors： Park SA,Park YS,Lee KS

更新日期：2014-07-01 00:00:00

abstract：:The amylosucrase encoding gene from Deinococcus geothermalis DSM 11300 (DgAS) was codon-optimized and expressed in Escherichia coli. The enzyme was employed for biosynthesis of three different dihydroxybenzene glucosides using sucrose as the source of glucose moiety. The reaction parameters, including temperature, pH,...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim TS,Parajuli P,Pandey RP,Sohng JK

更新日期：2018-09-28 00:00:00

abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...

journal_title：Journal of microbiology and biotechnology

authors： Park HJ,Min J,Ahn JM,Cho SJ,Ahn JY,Kim YH

更新日期：2015-02-01 00:00:00

abstract：:This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sung JY,Cho HH,Kwon KC,Koo SH

更新日期：2014-06-28 00:00:00

abstract：:Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-in...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Nam HJ,Kang JK,Seok H,Hwang JS,Kim H

更新日期：2014-05-01 00:00:00

abstract：:Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...

journal_title：Journal of microbiology and biotechnology

authors： Lee JM,Hwang KT,Jun WJ,Park CS,Lee MY

更新日期：2008-10-01 00:00:00

abstract：:A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel colu...

journal_title：Journal of microbiology and biotechnology

authors： Pak WM,Kim KB,Kim MJ,Cho JY,Ahn DH

更新日期：2015-03-01 00:00:00

abstract：:A cDNA encoding cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum and ChainA, stefin B te...

journal_title：Journal of microbiology and biotechnology

authors： Li S,Guo D,Zhao B,Ye J,Tian J,Ren W,Ju Y,Cui P,Li R

更新日期：2010-08-01 00:00:00

abstract：:Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells ...

journal_title：Journal of microbiology and biotechnology

authors： Kim SJ,Kim IW,Kwon YN,Yun EY,Hwang JS

更新日期：2012-02-01 00:00:00

abstract：:Cameleon is a genetically engineered Ca2+ sensing molecule consisting of two variants of the green fluorescent protein (GFP), calmodulin and calmodulin-binding protein, M13. HEK-293 cells stably expressing three types of cameleons, yellow cameleon-2, cameleon-3er, and cameleon-2nu, were constructed, and the expression...

journal_title：Journal of microbiology and biotechnology

authors： Ko HJ,Park TH

更新日期：2007-06-01 00:00:00

abstract：:In order to predict biologically significant attributes such as function from protein sequences, searching against large databases for homologous proteins is a common practice. In particular, BLAST and HMMER are widely used in a variety of biological fields. However, sequencehomologous proteins determined by BLAST and...

journal_title：Journal of microbiology and biotechnology

authors： Yu DS,Lee DH,Kim SK,Lee CH,Song JY,Kong EB,Kim JF

更新日期：2012-08-01 00:00:00

abstract：:In order to induce high levels of protein secretion, we have constructed a recombinant plasmid, designated pBP244, into which was incorporated key components of the type-II Sec-dependent secretion system, including LepB (signal peptidase), SecA (ATPase), and SecB (chaperone). The biological activities of the LepB, Sec...

journal_title：Journal of microbiology and biotechnology

authors： Kim SW,Kim YH,Yoo AY,Yu JE,Hur J,Lee JH,Cha J,Kang HY

更新日期：2007-08-01 00:00:00

abstract：:Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and termina...

journal_title：Journal of microbiology and biotechnology

authors： Park HD,Noguera DR

更新日期：2008-08-01 00:00:00