Functional analysis of olfactory receptors expressed in a HEK-293 cell system by using cameleons.

abstract：:An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50°C. The enzyme was stable between pH 6.0 and 11.0 at 25°C, 40°C, and 50°C for 24 h. The Km and Vmax of the enzy...

journal_title：Journal of microbiology and biotechnology

authors： Bakir ZB,Metin K

更新日期：2016-06-28 00:00:00

abstract：:Quorum sensing is a process of cell-to-cell communication in which bacteria produce autoinducers as signaling molecules to sense cell density and coordinate gene expression. In the present study, a LuxI-type synthase, AnoI, and a LuxR-type regulator, AnoR, were identified in Acinetobacter nosocomialis, an important no...

journal_title：Journal of microbiology and biotechnology

authors： Oh MH,Choi CH

更新日期：2015-08-01 00:00:00

abstract：:Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other met...

journal_title：Journal of microbiology and biotechnology

authors： Fatima M,Zaidi NU,Amraiz D,Afzal F

更新日期：2016-01-01 00:00:00

abstract：:In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test t...

journal_title：Journal of microbiology and biotechnology

authors： Pham HT,Tran HT,Vu LT,Dang HT,Nguyen TTT,Dang THT,Nguyen MTT,Nguyen HQ,Kim BH

更新日期：2019-07-28 00:00:00

abstract：:Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction proce...

journal_title：Journal of microbiology and biotechnology

authors： Nie Y,Xu Y,Hu QS,Xiao R

更新日期：2009-01-01 00:00:00

abstract：:Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essen...

journal_title：Journal of microbiology and biotechnology

authors： Chung KH,Yang KS,Kim J,Kim JC,Lee KY

更新日期：2007-11-01 00:00:00

abstract：:Soils amended for long-term with high levels of compost demonstrated greater abundance of bacterial members of the phylum Bacteroidetes whereas a decreasing trend in the relative abundance of phylum Acidobacteria was noted with increasing levels of compost. Metabolic profiles predicted by PICRUSt demonstrated differen...

journal_title：Journal of microbiology and biotechnology

authors： Samaddar S,Han GH,Chauhan P,Chatterjee P,Jeon S,Sa T

更新日期：2019-02-28 00:00:00

abstract：:Particle size and metal species are important to both soil microbial toxicity and phytotoxicity in the soil ecosystem. The effects of CuO and ZnO nanoparticles (NPs) and microparticles (MPs) on soil microbial toxicity, phytotoxicity, and bioaccumulation in two crops (Cucumis sativus and Zea mays) were estimated in a s...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sin H,Lee S,Lee I

更新日期：2013-09-28 00:00:00

abstract：:The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...

journal_title：Journal of microbiology and biotechnology

authors： Oh SW,Jang MU,Jeong CK,Kang HJ,Park JM,Kim TJ

更新日期：2008-08-01 00:00:00

abstract：:A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Ten IL,Jung HM,Liu QM,Im WT,Lee ST

更新日期：2008-06-01 00:00:00

abstract：:Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticu...

journal_title：Journal of microbiology and biotechnology

authors： Wang X,Huang Y,Sheng Y,Su P,Qiu Y,Ke C,Feng D

更新日期：2017-03-28 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children wi...

journal_title：Journal of microbiology and biotechnology

authors： Wen H,Yin X,Yuan Z,Wang X,Su S

更新日期：2018-04-28 00:00:00

abstract：:The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and 37 degrees C and microaerobic (O2 5%, CO2 10%, N2 85%) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morp...

journal_title：Journal of microbiology and biotechnology

authors： Jang KI,Kim MG,Ha SD,Kim KS,Lee KH,Chung DH,Kim CH,Kim KY

更新日期：2007-02-01 00:00:00

abstract：:Areca nut (AN) chewing is a habit in many countries in Central, Southern, and Southeast Asia. It is strongly associated with the occurrence of oral, pharyngeal, and esophageal cancer as well as systemic inflammation. However, the association between AN intake and the development of gastric lesions has not yet been ide...

journal_title：Journal of microbiology and biotechnology

authors： Lee J,Gunawardhana ND,Jang S,Choi YH,Illeperuma RP,Kim A,Su H,Hong YA,Kim JH,Kim J,Jung DW,Cha IH,Bak EJ,Cha JH

更新日期：2016-10-28 00:00:00

abstract：:Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity ...

journal_title：Journal of microbiology and biotechnology

authors： Lin X,Liu S,Xie G,Chen J,Li P,Chen J

更新日期：2016-11-28 00:00:00

abstract：:The evolution of living organisms occurs via a combination of highly complicated processes that involve modification of various features such as appearance, metabolism and sensing systems. To understand the evolution of life, it is necessary to understand how each biological feature has been optimized in response to n...

journal_title：Journal of microbiology and biotechnology

authors： Nguyen TV,Hong SH,Lee SY

更新日期：2009-11-01 00:00:00

abstract：:Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...

journal_title：Journal of microbiology and biotechnology

authors： Yang SJ,Ko JA,Kim HS,Jo MH,Lee HN,Park BR,Kim Y

更新日期：2018-12-28 00:00:00

abstract：:Two alternative pathways for methionine biosynthesis are known in Corynebacterium glutamicum: one involving transsulfuration (mediated by metB and metC) and the other involving direct sulthydrylation (mediated by metY). In this study, MetB (cystathionine gamma-synthase) and MetY (O-acetylhomoserine sulfhydrylase) from...

journal_title：Journal of microbiology and biotechnology

authors： Hwang BJ,Park SD,Kim Y,Kim P,Lee HS

更新日期：2007-06-01 00:00:00

abstract：:Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, ...

journal_title：Journal of microbiology and biotechnology

authors： Khalmuratova I,Choi DH,Woo JR,Jeong MJ,Oh Y,Kim YG,Lee IJ,Choo YS,Kim JG

更新日期：2020-11-28 00:00:00

abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...

journal_title：Journal of microbiology and biotechnology

authors： Long D,Tang X,Cai K,Chen G,Shen C,Shi J,Chen L,Chen Y

更新日期：2013-08-01 00:00:00

abstract：:A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75 de...

journal_title：Journal of microbiology and biotechnology

authors： Liu B,Zhang N,Zhao C,Lin B,Xie L,Huang Y

更新日期：2012-10-01 00:00:00

abstract：:Beta-1,3-glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. beta-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of t...

journal_title：Journal of microbiology and biotechnology

authors： Shim JH,Sung KJ,Cho MC,Choi WA,Yang Y,Lim JS,Yoon DY

更新日期：2007-09-01 00:00:00

abstract：:In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...

journal_title：Journal of microbiology and biotechnology

authors： Mashhadi-Karim M,Azin M,Gargari SL

更新日期：2011-12-01 00:00:00

abstract：:The metabolic engineering of epothilones, as secondary metabolites, was investigated using Sorangium cellulosum to achieve the selective production of epothilone B, a potent anticancer agent. Thus, the propionyl-CoA synthetase gene (prpE) from Ralstonia solanacearum was heterologously expressed in S. cellulosum to inc...

journal_title：Journal of microbiology and biotechnology

authors： Han SJ,Park SW,Park BW,Sim SJ

更新日期：2008-01-01 00:00:00

abstract：:Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms....

journal_title：Journal of microbiology and biotechnology

authors： Kim JE,Monmai C,Rod-In W,Jang AY,You SG,Lee SM,Park WJ

更新日期：2019-09-28 00:00:00

abstract：:Outer membrane vesicles (OMVs) are spherical nanostructures that are ubiquitously shed from gram-negative bacteria both in vitro and in vivo. Recent findings revealed that OMVs, which contain diverse components derived from the parent bacterium, play an important role in communication with neighboring bacteria and the...

journal_title：Journal of microbiology and biotechnology

authors： Yoon H

更新日期：2016-08-28 00:00:00

abstract：:It has been well known that the use of Saccharomyces cerevisiae can cause fungemia in critically ill patients and flavone shows an antimicrobial effect on S. cerevisiae. Therefore, we have investigated the activities of thirteen flavone analogues on S. cerevisiae in our studies. Because flavonoids including flavones h...

journal_title：Journal of microbiology and biotechnology

authors： Young J,Park Y,Lee YU,Kim H,Shim YH,Ahn JH,Lim Y

更新日期：2007-03-01 00:00:00

abstract：:Scientific research regarding the use of live bacterial cells for therapeutic purposes has been rapidly growing over the years and has generated considerable interest to scientists and health professionals. Probiotics are defined as essential live microorganisms which, when administered in adequate amounts, confer a h...

journal_title：Journal of microbiology and biotechnology

authors： Islam MA,Yun CH,Choi YJ,Cho CS

更新日期：2010-10-01 00:00:00

abstract：:The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

journal_title：Journal of microbiology and biotechnology

authors： Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

更新日期：2013-08-01 00:00:00