Abstract：
:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activity increased in the presence of 30% methanol and, remarkably, increased as well to 154.6% in the presence of Ca(2+). Lip7-3 preferred pnitrophenyl octanoate (C8) as a substrate and exhibited broad specificity for short- to longchain fatty acid esters. Additionally, Lip7-3 showed a low degree of enantioselectivity for an S-enantiomer (e.g., (S)-methyl-3-hydroxy-2-methylpropionate). It efficiently hydrolyzed glyceryl tributyrate, but did not hydrolyze glyceryl trioleate, fish oil, or olive oil. Its substrate specificity and activation by the solvent might offer a merit to the biotechnological enzyme applications like transesterification in the production of biodiesel.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Kim HJ,Jung WK,Lee HW,Yoo W,Kim TD,Kim Hdoi
10.4014/jmb.1507.07104subject
Has Abstractpub_date
2015-12-28 00:00:00pages
2024-33issue
12eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1507.07104journal_volume
25pub_type
杂志文章abstract：:This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1502.02057
更新日期：2015-10-01 00:00:00
abstract：:Organic solvent-tolerant (OST) enzymes are widely applied in various industries due to their activity and stability in organic solvents, higher substrate solubility, and increased stereo-selectivity. However, the criteria for identifying OST enzymes largely remain unresolved. In this study, we compared the amino acid ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1806.06032
更新日期：2018-09-28 00:00:00
abstract：:In recent years, foot-and-mouth disease has occurred in all parts of the world. The animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. Moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1603.03056
更新日期：2016-08-28 00:00:00
abstract：:Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-10-01 00:00:00
abstract：:This study examined the laxative effects of hot-water extracts of Hovenia dulcis Thunb. (HD), Phyllostachys pubescens Mazel (PM), and a 2:8 mixture of both (HP) in two chronic constipation models. For the loperamide-induced constipation model, animals were divided into an untreated group, negative control group (loper...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1911.11051
更新日期：2020-05-28 00:00:00
abstract：:In this study, we compared the virulence-associated factors of Acinetobacter baumannii complex species. Sixty-three isolates of five A. baumannii complex species, including 19 A. baumannii, 15 A. nosocomialis, 13 A. seifertii, 13 A. pittii, and 3 A. calcoaceticus isolates, were included in this study. For all isolates...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1507.07076
更新日期：2016-01-01 00:00:00
abstract：:In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbo...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1003.03022
更新日期：2010-09-01 00:00:00
abstract：:Staphylococcus aureus strains are important foodborne pathogens that produce various toxins. To evaluate the risk of the enterotoxins, four S. aureus strains from kinbap and two clinical samples were isolated and identified, and their expression of the enterotoxin genes were analyzed using a reverse transcription real...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:A Gram-positive and endospore-forming strain, JH8T, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176T (96.9%...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-02-01 00:00:00
abstract：:Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1707.07007
更新日期：2017-11-28 00:00:00
abstract：:Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center f...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1212.12029
更新日期：2013-05-01 00:00:00
abstract：:Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×10(7) CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1208.08018
更新日期：2013-02-01 00:00:00
abstract：:Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange c...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-04-01 00:00:00
abstract：:Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-s...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-11-01 00:00:00
abstract：:Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biol...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1501.01006
更新日期：2015-04-01 00:00:00
abstract：:During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3- hydroxypropionaldehyde to 1,3-propanediol using NADP...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1307.07015
更新日期：2013-12-01 00:00:00
abstract：:The impact of overproduction of a heterologous protein on the metabolic system of host Lactococcus lactis was investigated. The protein expression profiles of L. lactis IL1403 containing two near-identical plasmids that expressed high- and low-level of the green fluorescent protein (GFP) were examined via shotgun prot...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1703.03065
更新日期：2017-07-28 00:00:00
abstract：:Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after t...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1505.05062
更新日期：2015-11-01 00:00:00
abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1410.10023
更新日期：2015-02-01 00:00:00
abstract：:Monoamine oxidase (MAO) is found in most cell types and catalyzes the oxidation of monoamines. Three anithiactins (A-C, modified 2-phenylthiazoles) isolated from Streptomyces sp. were tested for inhibitory activity of two isoforms, MAO-A and MAO-B. Anithiactin A was effective and selective for the inhibition of MAO-A,...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1505.05020
更新日期：2015-09-01 00:00:00
abstract：:Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children wi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1711.11065
更新日期：2018-04-28 00:00:00
abstract：:Rumex japonicus Houtt (RJH) is a valuable plant used in traditional medicine to treat several diseases, such as scabies and jaundice. In this study, Jurkat cell growth inhibitory extracts of R. japonicus roots were subjected to bioassay-guided fractionation, resulting in the isolation of three naphthalene derivatives ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.2007.07018
更新日期：2020-12-28 00:00:00
abstract：:The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for lar...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1610.10037
更新日期：2017-01-28 00:00:00
abstract：:Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanopa...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1608.08011
更新日期：2017-02-28 00:00:00
abstract：:Lactobacillus brevis ATCC 14869 exhibited a carbon catabolite de-repressed (CCR) phenotype which has ability to consume fermentable sugar simultaneously with glucose. To evaluate this unusual phenotype under harsh conditions during fermentation, the effect of lactic acid and hydrogen ion concentrations on L. brevis AT...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1512.12038
更新日期：2016-07-28 00:00:00
abstract：:Glucose dehydrogenase (GDH) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. In this study, the glucose 1-dehydrogenase B gene (gdhB) was cloned from Bacillus thuringiensis subsp. kurstaki, and wild-type (GDH-BTWT) and His-tagged (GDH-BTN-...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1603.03021
更新日期：2016-10-28 00:00:00
abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1407.07001
更新日期：2015-01-01 00:00:00
abstract：:The effects of algal inoculation on chemical oxygen demand (COD) and total nitrogen (TN) removal, and indigenous bacterial dynamics were investigated in municipal wastewater. Experiments were conducted with municipal wastewater inoculated with either Chlorella vulgaris AG10032, Selenastrum gracile UTEX 325, or Scenede...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1512.12067
更新日期：2016-05-28 00:00:00
abstract：:In the bacterial community, unicellular organisms act together as a multicellular being. Bacteria interact within the community and programmed cell death (PCD) in prokaryotes is a sort of altruistic action that enables the whole population to thrive. Genetically, encoded cell death pathways are triggered by DNA damage...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.1904.04017
更新日期：2019-07-28 00:00:00
abstract：:Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from Paeonia lactiflora against Candida albicans, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabli...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1611.11064
更新日期：2017-02-28 00:00:00
