Abstract：
:Organic solvent-tolerant (OST) enzymes are widely applied in various industries due to their activity and stability in organic solvents, higher substrate solubility, and increased stereo-selectivity. However, the criteria for identifying OST enzymes largely remain unresolved. In this study, we compared the amino acid composition of 19 OST esterases and 19 non-OST esterases. OST esterases have increased ratio of Ala and Arg residues and decreased ratio of Asn, Ile, Tyr, and Ser residues. Based on the amino acid composition analysis, we cloned acarboxylesterase (EstSP2) from a psychrophilic bacterium, Sphingomonas glacialis PAMC 26605, and characterized its recombinant protein. EstSP2 is substrate specific to p-nitrophenyl acetate and hydrolyzed aspirin, with optimal activityat 40°C; at 4°C, the activity is approximately 50% of its maximum. As expected, EstSP2showstolerance in up to 40% concentration of polar organic solvents, including dimethyl sulfoxide, methanol, and ethanol. The results of this study suggest that selection of OST esterases based on their amino acid composition analysis could be a novel approach to identify OST esterases produced from bacterial genomes.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Dachuri VK,Lee C,Jang SHdoi
10.4014/jmb.1806.06032subject
Has Abstractpub_date
2018-09-28 00:00:00pages
1502-1510issue
9eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1806.06032journal_volume
28pub_type
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journal_title：Journal of microbiology and biotechnology
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abstract：:The effects of algal inoculation on chemical oxygen demand (COD) and total nitrogen (TN) removal, and indigenous bacterial dynamics were investigated in municipal wastewater. Experiments were conducted with municipal wastewater inoculated with either Chlorella vulgaris AG10032, Selenastrum gracile UTEX 325, or Scenede...
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更新日期：2008-05-01 00:00:00
abstract：:Ahead of Print article withdrawn by publisher. ...
journal_title：Journal of microbiology and biotechnology
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更新日期：2009-10-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1308.08007
更新日期：2013-11-28 00:00:00
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journal_title：Journal of microbiology and biotechnology
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doi：10.4014/jmb.1505.05062
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.2002.02032
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-05-01 00:00:00
abstract：:The past decades have been a golden era during which great tasks were accomplished in the field of microbiology, including food microbiology. In the past, culture-dependent methods have been the primary choice to investigate bacterial diversity. However, using cultureindependent high-throughput sequencing of 16S rRNA ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
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