Synthetic Bacteria for Therapeutics.

abstract：:In the present work, current knowledge on the potential fate, microbial degradation, and toxicity of hexahydro- 1,3,5-trinitro-1,3,5-triazine (RDX) was thoroughly reviewed, focusing on the toxicological assessment of a variety of potential RDX degradation pathways in bacteria and fungi. The present review on microbial...

journal_title：Journal of microbiology and biotechnology

authors： Khan MI,Lee J,Park J

更新日期：2012-10-01 00:00:00

abstract：:Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline ...

journal_title：Journal of microbiology and biotechnology

authors： Ha JS,Lee YM,Choi SL,Song JJ,Shin CS,Kim JH,Lee SG

更新日期：2008-03-01 00:00:00

abstract：:Gibberellins (GAs) are a group of phytohormones that control many developmental processes in higher plants. We report the cloning and expression pattern of gibberellin biosynthesis genes from a new GA-producing fungus, Fusarium proliferatum (strain KGL0401). These genes sequences are deposited in the National Center f...

journal_title：Journal of microbiology and biotechnology

authors： Rim SO,You YH,Yoon H,Kim YE,Lee JH,Kang MS,Kim C,Seu YB,Kim JG

更新日期：2013-05-01 00:00:00

abstract：:The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for th...

journal_title：Journal of microbiology and biotechnology

authors： Kim JY,Kim HK,Jang HJ,Kim EK,Kim MK

更新日期：2015-04-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized DEAE·HCl derivatization...

journal_title：Journal of microbiology and biotechnology

authors： Lee SE,Lee CG,Kang DH,Lee HY,Jung KH

更新日期：2012-12-01 00:00:00

abstract：:A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced i...

journal_title：Journal of microbiology and biotechnology

authors： Khang YH,Park HY,Jeong YS,Kim JA,Kim YH

更新日期：2009-05-01 00:00:00

abstract：:Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by a...

journal_title：Journal of microbiology and biotechnology

authors： Ho CW,Tan WS,Chong FC,Ling TC,Tey BT

更新日期：2009-04-01 00:00:00

abstract：:In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...

journal_title：Journal of microbiology and biotechnology

authors： Mashhadi-Karim M,Azin M,Gargari SL

更新日期：2011-12-01 00:00:00

abstract：:Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosy...

journal_title：Journal of microbiology and biotechnology

authors： Pranawidjaja S,Choi SI,Lay BW,Kim P

更新日期：2015-06-01 00:00:00

abstract：:The aim of this work is to investigate the protective efficacy of emodin, an active, naturally-occurring anthraquinone derivative of several traditional Chinese herbs, against Brucella abortus infection in macrophages. Brucella were incubated with different concentrations of emodin and showed that bacterial survival r...

journal_title：Journal of microbiology and biotechnology

authors： Huy TXN,Reyes AWB,Hop HT,Arayan LT,Son VH,Min W,Lee HJ,Kim S

更新日期：2018-10-28 00:00:00

abstract：:We previously reported that the CopA3 peptide (LLCIALRKK, D-form) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the D-form, has limited the development of CopA3 for therapeutic purposes. Here, we in...

journal_title：Journal of microbiology and biotechnology

authors： Yoon IN,Hong J,Zhang P,Hwang JS,Kim H

更新日期：2017-02-28 00:00:00

abstract：:Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...

journal_title：Journal of microbiology and biotechnology

authors： Oh KH,Cho SH

更新日期：2014-03-28 00:00:00

abstract：:Four series (S, M, R, and W) of Alternaria longipes isolates were obtained based on consecutive induction with Dimethachlon (Dim) and ultraviolet irradiation. These isolates were then characterized according to their tolerance to Dim, sensitivity to osmotic stress, and phenotypic properties. All the induced Dim-resist...

journal_title：Journal of microbiology and biotechnology

authors： Luo Y,Yang J,Zhu M,Yan J,Mo M,Zhqng K

更新日期：2008-01-01 00:00:00

abstract：:This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory con...

journal_title：Journal of microbiology and biotechnology

authors： Cha JD,Jeong MR,Jeong SI,Lee KY

更新日期：2007-05-01 00:00:00

abstract：:The cohesin complex holds sister chromatids together and prevents premature chromosome segregation until the onset of anaphase. Mcd1 (also known as Scc1), the α-kleisin subunit of cohesin, is a key regulatory subunit of the mitotic cohesin complex and is required for maintaining sister chromatid cohesion, chromosome o...

journal_title：Journal of microbiology and biotechnology

authors： Lee MS,Yoon SW,Kim KP

更新日期：2015-05-01 00:00:00

abstract：:Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...

journal_title：Journal of microbiology and biotechnology

authors： Cheon SH,Lee KH,Kwon JY,Ryu HN,Yu DH,Choi YS,Kim DI

更新日期：2007-12-01 00:00:00

abstract：:In order to predict biologically significant attributes such as function from protein sequences, searching against large databases for homologous proteins is a common practice. In particular, BLAST and HMMER are widely used in a variety of biological fields. However, sequencehomologous proteins determined by BLAST and...

journal_title：Journal of microbiology and biotechnology

authors： Yu DS,Lee DH,Kim SK,Lee CH,Song JY,Kong EB,Kim JF

更新日期：2012-08-01 00:00:00

abstract：:During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3- hydroxypropionaldehyde to 1,3-propanediol using NADP...

journal_title：Journal of microbiology and biotechnology

authors： Ma CW,Zhang L,Dai JY,Xiu ZL

更新日期：2013-12-01 00:00:00

abstract：:Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essen...

journal_title：Journal of microbiology and biotechnology

authors： Chung KH,Yang KS,Kim J,Kim JC,Lee KY

更新日期：2007-11-01 00:00:00

abstract：:Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF ...

journal_title：Journal of microbiology and biotechnology

authors： Kong MK,Min BH,Lee PC

更新日期：2012-05-01 00:00:00

abstract：:Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 days. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and...

journal_title：Journal of microbiology and biotechnology

authors： Lü Y,Gong Y,Li Y,Pan Z,Yao Y,Li N,Guo J,Gong D,Tian Y,Peng C

更新日期：2017-08-28 00:00:00

abstract：:A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA ...

journal_title：Journal of microbiology and biotechnology

authors： Rajkumar R,Kothilmozhian J,Ramasamy R

更新日期：2011-06-01 00:00:00

abstract：:Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other related conditions. In this study, we investigated the antimelanogenic effects of an extract from the microalgae Chlamydomonas reinhardtii (CE) and potential mechanisms responsible for its inhibi...

journal_title：Journal of microbiology and biotechnology

authors： Lee A,Kim JY,Heo J,Cho DH,Kim HS,An IS,An S,Bae S

更新日期：2018-12-28 00:00:00

abstract：:Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobiliz...

journal_title：Journal of microbiology and biotechnology

authors： Yao J,Chen Q,Zhong G,Cao W,Yu A,Liu Y

更新日期：2014-01-01 00:00:00

abstract：:Alcohol dependence is a global public health problem, yet the mechanisms of alcohol dependence are incompletely understood. The traditional view has been that ethanol alters various neurotransmitters and their receptors in the brain and causes the addiction. However, an increasing amount of experimental evidence sugge...

journal_title：Journal of microbiology and biotechnology

authors： Fan Y,Ya-E Z,Ji-Dong W,Yu-Fan L,Ying Z,Ya-Lun S,Meng-Yu M,Rui-Ling Z

更新日期：2018-11-28 00:00:00

abstract：:Polyester cloth (PC) was selected as a prospective inexpensive substitute separator material for microbial fuel cells (MFCs). PC was compared with a traditional Nafion proton exchange membrane (PEM) as an MFC separator by analyzing its physical and electrochemical properties. A single layer of PC showed higher mass tr...

journal_title：Journal of microbiology and biotechnology

authors： Kim T,Kang S,Sung JH,Kang YK,Kim YH,Jang JK

更新日期：2016-12-28 00:00:00

abstract：:In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipene...

journal_title：Journal of microbiology and biotechnology

authors： Han AW,Oh KH,Jheong WH,Cho YC

更新日期：2010-07-01 00:00:00

abstract：:Many strains of Bacillus cereus cause gastrointestinal diseases, and the closely related insect pathogen Bacillus thuringiensis has also been involved in outbreaks of diarrhea. The diarrheal diseases are attributed to enterotoxins. Sixteen reference strains of B. cereus and nine commercial and 12 reference strains of ...

journal_title：Journal of microbiology and biotechnology

authors： Kim MJ,Han JK,Park JS,Lee JS,Lee SH,Cho JI,Kim KS

更新日期：2015-06-01 00:00:00

abstract：:The correlation between alcoholic fermentation rate, measured as carbon dioxide (CO2) evolution, and the rate of hydrogen sulfide (H2S) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace H2S were directly impacted by yeast assimilable nitrogenous ...

journal_title：Journal of microbiology and biotechnology

authors： Butzke CE,Park SK

更新日期：2011-05-01 00:00:00