Biotransformation of Glycosylated Saponins in Balloon Flower Root Extract into 3-O-β-D-Glucopyranosyl Platycosides by Deglycosylation of Pectinase from Aspergillus aculeatus.

abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Xu Y,Chen X,Zhang LY

更新日期：2015-01-01 00:00:00

abstract：:Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus T...

journal_title：Journal of microbiology and biotechnology

authors： Jirasatid S,Nopharatana M,Kitsubun P,Vichitsoonthonkul T,Tongta A

更新日期：2013-03-01 00:00:00

abstract：:The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and 37 degrees C and microaerobic (O2 5%, CO2 10%, N2 85%) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morp...

journal_title：Journal of microbiology and biotechnology

authors： Jang KI,Kim MG,Ha SD,Kim KS,Lee KH,Chung DH,Kim CH,Kim KY

更新日期：2007-02-01 00:00:00

abstract：:The amylosucrase encoding gene from Deinococcus geothermalis DSM 11300 (DgAS) was codon-optimized and expressed in Escherichia coli. The enzyme was employed for biosynthesis of three different dihydroxybenzene glucosides using sucrose as the source of glucose moiety. The reaction parameters, including temperature, pH,...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim TS,Parajuli P,Pandey RP,Sohng JK

更新日期：2018-09-28 00:00:00

abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...

journal_title：Journal of microbiology and biotechnology

authors： Wang J,Wang ZY,He XP,Zhang BR

更新日期：2010-11-01 00:00:00

abstract：:A Gram-positive and endospore-forming strain, JH8T, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176T (96.9%...

journal_title：Journal of microbiology and biotechnology

authors： Choi JH,Im WT,Yoo JS,Lee SM,Moon DS,Kim HJ,Rhee SK,Roh DH

更新日期：2008-02-01 00:00:00

abstract：:In recent years, foot-and-mouth disease has occurred in all parts of the world. The animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. Moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans...

journal_title：Journal of microbiology and biotechnology

authors： Kim BN,Cho HS,Cha Y,Park JK,Kim G,Kim YH,Min J

更新日期：2016-08-28 00:00:00

abstract：:VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast c...

journal_title：Journal of microbiology and biotechnology

authors： Luo S,Yan L,Zhang X,Yuan L,Fang Q,Zhang YA,Dai H

更新日期：2015-12-28 00:00:00

abstract：:The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALN...

journal_title：Journal of microbiology and biotechnology

authors： Hernández A,Velásquez O,Leonardi F,Soto C,Rodríguez A,Lizaraso L,Mosquera Á,Bohórquez J,Coronado A,Espejo Á,Sierra R,Sánchez OF,Alméciga-Díaz CJ,Barrera LA

更新日期：2013-05-01 00:00:00

abstract：:A gene (acas) designated as alpha-amylase was cloned from Arthrobacter chlorophenolicus A6. The multiple amino acid sequence analysis and functional expression of acas revealed that this gene really encoded an amylosucrase (ASase) instead of alpha-amylase. In fact, the recombinant enzyme exhibited typical ASase activi...

journal_title：Journal of microbiology and biotechnology

authors： Seo DH,Jung JH,Choi HC,Cho HK,Kim HH,Ha SJ,Yoo SH,Cha J,Park CS

更新日期：2012-09-01 00:00:00

abstract：:We previously reported that the CopA3 peptide (LLCIALRKK, D-form) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the D-form, has limited the development of CopA3 for therapeutic purposes. Here, we in...

journal_title：Journal of microbiology and biotechnology

authors： Yoon IN,Hong J,Zhang P,Hwang JS,Kim H

更新日期：2017-02-28 00:00:00

abstract：:L-Arabinose, a five carbon sugar, has not been considered as an important bioresource because most studies have focused on D-xylose, another type of five-carbon sugar that is prevalent as a monomeric structure of hemicellulose. In fact, L-arabinose is also an important monomer of hemicellulose, but its content is much...

journal_title：Journal of microbiology and biotechnology

authors： Ye S,Kim JW,Kim SR

更新日期：2019-03-28 00:00:00

abstract：:During menopausal transition, the imbalance of estrogen causes body weight gain. Although gut microbiome dysbiosis has been reported in postmenopausal obesity, it is not clear whether there is any difference in the microbiome profile between dietary-induced obesity and postmenopausal obesity. Therefore, in this study,...

journal_title：Journal of microbiology and biotechnology

authors： Choi S,Hwang YJ,Shin MJ,Yi H

更新日期：2017-12-28 00:00:00

abstract：:Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity ...

journal_title：Journal of microbiology and biotechnology

authors： Lin X,Liu S,Xie G,Chen J,Li P,Chen J

更新日期：2016-11-28 00:00:00

abstract：:The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protectio...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim Y

更新日期：2020-07-28 00:00:00

abstract：:The evolution of living organisms occurs via a combination of highly complicated processes that involve modification of various features such as appearance, metabolism and sensing systems. To understand the evolution of life, it is necessary to understand how each biological feature has been optimized in response to n...

journal_title：Journal of microbiology and biotechnology

authors： Nguyen TV,Hong SH,Lee SY

更新日期：2009-11-01 00:00:00

abstract：:Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. ga...

journal_title：Journal of microbiology and biotechnology

authors： Xu X,Feng L,Han Z,Luo S,Wu A,Xie J

更新日期：2016-09-28 00:00:00

abstract：:Ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate((S)-HEES)acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which canbe achieved viathe stereoselective bioreduction ofethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that containsa 3-oxoacyl structure.The sequences of the short-chain dehydroge...

journal_title：Journal of microbiology and biotechnology

authors： Ren ZQ,Liu Y,Pei XQ,Wu ZL

更新日期：2019-11-28 00:00:00

abstract：:Monoamine oxidase (MAO) is found in most cell types and catalyzes the oxidation of monoamines. Three anithiactins (A-C, modified 2-phenylthiazoles) isolated from Streptomyces sp. were tested for inhibitory activity of two isoforms, MAO-A and MAO-B. Anithiactin A was effective and selective for the inhibition of MAO-A,...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Jung WK,Kim HJ,Jeong YS,Nam SJ,Kang H,Kim H

更新日期：2015-09-01 00:00:00

abstract：:Arbuscular mycorrhizal fungi (AMF) have great potential for assisting heavy metal hyperaccumulators in the remediation of contaminated soils. However, little information is available about the symbiosis of AMF associated with an antimony (Sb) accumulator plant under natural conditions. Therefore, the objective of this...

journal_title：Journal of microbiology and biotechnology

authors： Wei Y,Chen Z,Wu F,Li J,ShangGuan Y,Li F,Zeng QR,Hou H

更新日期：2015-08-01 00:00:00

abstract：:Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogenicity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, and 634...

journal_title：Journal of microbiology and biotechnology

authors： Rosas-García NM,Avalos-de-León O,Villegas-Mendoza JM,Mireles-Martínez M,Barboza-Corona JE,Castañeda-Ramírez JC

更新日期：2014-11-28 00:00:00

abstract：:Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...

journal_title：Journal of microbiology and biotechnology

authors： Park IH,Kim SH,Lee YS,Lee SC,Zhou Y,Kim CM,Ahn SC,Choi YL

更新日期：2009-02-01 00:00:00

abstract：:The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

journal_title：Journal of microbiology and biotechnology

authors： Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

更新日期：2013-08-01 00:00:00

abstract：:TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic alpha- helical barrel domain and a membrane-embedded beta-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and dr...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Yum S,Jo WS,Lee BL,Jeong MH,Ha NC

更新日期：2008-05-01 00:00:00

abstract：:Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-s...

journal_title：Journal of microbiology and biotechnology

authors： Ahn JO,Lee HW,Saha R,Park MS,Jung JK,Lee DY

更新日期：2008-11-01 00:00:00

abstract：:Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms....

journal_title：Journal of microbiology and biotechnology

authors： Kim JE,Monmai C,Rod-In W,Jang AY,You SG,Lee SM,Park WJ

更新日期：2019-09-28 00:00:00

abstract：:The purified endochitosanase (Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers (GlcN)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this e...

journal_title：Journal of microbiology and biotechnology

authors： Gao XA,Jung WJ,Kuk JH,Park RD

更新日期：2009-04-01 00:00:00

abstract：:An agar-degrading bacterium, designated as strain G7(T), was isolated from a coastal seawater sample from Gaya Island (Gayado in Korean), Republic of Korea. The isolated strain G7(T) is gram-negative, rod shaped, aerobic, non-motile, and non-pigmented. A similarity search based on its 16S rRNA gene sequence revealed t...

journal_title：Journal of microbiology and biotechnology

authors： Chi WJ,Park JS,Kwak MJ,Kim JF,Chang YK,Hong SK

更新日期：2013-11-28 00:00:00

abstract：:Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS...

journal_title：Journal of microbiology and biotechnology

authors： Kim JG,Kim MJ,Lee JS,Sydara K,Lee S,Byun S,Jung SK

更新日期：2020-06-28 00:00:00

abstract：:An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50°C. The enzyme was stable between pH 6.0 and 11.0 at 25°C, 40°C, and 50°C for 24 h. The Km and Vmax of the enzy...

journal_title：Journal of microbiology and biotechnology

authors： Bakir ZB,Metin K

更新日期：2016-06-28 00:00:00