Abstract：
:Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosalicylic acid (DNS) method for microwell plate-based screening. Targeted microbial samples were initially cultured on agar plates with both cellulose and xylan as substrates. Then, isolated colonies were subcultured in broth media containing yeast extract and either cellulose or xylan. The supernatants of the culture broth were tested with our modified DNS screening method in a 96-microwell plate, with a 200 μl total reaction volume. In addition, the stability and reliability of glucose and xylose standards, which were used to determine the enzymatic activity, were studied at 100°C for different time intervals in a dry oven. It was concluded that the minimum incubation time required for stable color development of the standard solution is 20 min. With this technique, we successfully screened 21 and 31 cellulase- and xylanase-producing strains, respectively, in a single experimental trial. Among the identified strains, 19 showed both cellulose and xylan hydrolyzing activities. These microbes can be applied to bioethanol production from cellulosic and hemicellulosic biomass.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh Cdoi
10.4014/jmb.1405.05052subject
Has Abstractpub_date
2014-11-28 00:00:00pages
1559-65issue
11eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1405.05052journal_volume
24pub_type
杂志文章abstract：:Communication between endothelial cells (ECs) and smooth muscle cells (SMCs) via miR-143/145 clusters is vital to vascular stability. Previous research demonstrates that miR-143/145 released from ECs can regulate SMC proliferation and migration. In addition, a recent study has found that SMCs also have the capability ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1507.07103
更新日期：2016-03-01 00:00:00
abstract：:Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...
journal_title：Journal of microbiology and biotechnology
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更新日期：2018-12-28 00:00:00
abstract：:To date, there has been no employment of a magnetoelastic (ME) biosensor method to detect Salmonella enterica serovar Typhimurium in soil. The ME biosensor method needs to be investigated and modified for its successful performance. The filtration method, cation-exchange resin method, and combinations of both methods ...
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abstract：:Bacterial resistance to antibiotics has been emerged as a major health problem in the recent years. Silver nanoparticles (AgNPs) have been known to have an inhibitory effect to the bacterial growth, however, the strength of this antibacterial activity in correlation with the physical properties of these AgNPs has bare...
journal_title：Journal of microbiology and biotechnology
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更新日期：2018-07-19 00:00:00
abstract：:A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel colu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1409.09012
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abstract：:Optimal conditions for a high cell density fermentation were investigated in a recombinant Escherichia coli producing salmosin, a platelet aggregation inhibitor. The optimized carbon and nitrogen sources were glycerol 10 g/l, yeast extract 30 g/l, and bacto-tryptone 10 g/l, yielding the dry cell weight (DCW) of 10.61 ...
journal_title：Journal of microbiology and biotechnology
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doi：10.4014/jmb.1105.05014
更新日期：2011-10-01 00:00:00
abstract：:The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups o...
journal_title：Journal of microbiology and biotechnology
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更新日期：2011-02-01 00:00:00
abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...
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doi：10.4014/jmb.1301.01004
更新日期：2013-08-01 00:00:00
abstract：:An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...
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更新日期：2012-01-01 00:00:00
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doi：10.4014/jmb.1407.07072
更新日期：2014-11-28 00:00:00
abstract：:Colorectal cancer (CRC) is regarded as one of the most common and deadly forms of cancer. Gut microbiota is vital to retain and promote several functions of intestinal. Although previous researches have shown that some gut microbiota have the abilities to inhibit tumorigenesis and prevent cancer from progressing, they...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.2002.02032
更新日期：2020-11-28 00:00:00
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journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2008-07-01 00:00:00
abstract：:Accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. To investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1409.09085
更新日期：2015-05-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.1203.04002
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abstract：:Enterobacter sakazakii may be related to outbreaks of meningitis, septicemia, and necrotizing enterocolitis, mainly in neonates. To reduce the risk of E. sakazakii in baby foods, thermal characteristics for Korean E. sakazakii isolates were determined at 52, 56, and 60 degrees C in saline solution, rehydrated powdered...
journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2007-02-01 00:00:00
abstract：:Three different polysaccharide-peptide complexes (PPC, named as Fr-I, Fr-II, and Fr-III) were produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala, and their anticancer activities were investigated in human hepatocarcinoma (HepG2) and neuroblastoma (SK-N-SH) cells. The highest i...
journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2008-03-01 00:00:00
abstract：:Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...
journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2014-03-28 00:00:00
abstract：:Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus T...
journal_title：Journal of microbiology and biotechnology
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doi：10.4014/jmb.1206.06068
更新日期：2013-03-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-03-01 00:00:00
abstract：:Beta-1,3-glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. beta-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of t...
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更新日期：2007-09-01 00:00:00
abstract：:The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The alpha-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a...
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更新日期：2010-12-01 00:00:00
abstract：:Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possibl...
journal_title：Journal of microbiology and biotechnology
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更新日期：2014-05-01 00:00:00
abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen that produces and secretes exopolysaccharides (EPS), in which cells are embedded to form a highly organized community structure called biofilm. Here, we characterized the role of cyclic diguanylate (c-di-GMP) and EPS (PEL) overproduction in the wspF mutant phen...
journal_title：Journal of microbiology and biotechnology
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更新日期：2008-07-01 00:00:00
abstract：:The thermophile Bacillus fordii MH602 was screened for stereospecifically hydrolyzing DL-5-substituted hydantoins to L-alpha-amino acids. Since the reaction at higher temperature, the advantageous for enhancement of substrate solubility and for racemization of DL-5-substituted hydantoins during the conversion were ach...
journal_title：Journal of microbiology and biotechnology
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更新日期：2007-06-01 00:00:00
abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1507.07104
更新日期：2015-12-28 00:00:00
abstract：:Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2008-10-01 00:00:00
abstract：:Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CYPVdh, and CYPeryF, as templates. The lowest energy SPCYP147F1 model was then assessed for stereochemical quality and side-chain environment by Accelrys Discovery Studio 3.1 software. Further actives...
journal_title：Journal of microbiology and biotechnology
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更新日期：2012-07-01 00:00:00
