Abstract:
:Bacterial resistance to antibiotics has been emerged as a major health problem in the recent years. Silver nanoparticles (AgNPs) have been known to have an inhibitory effect to the bacterial growth, however, the strength of this antibacterial activity in correlation with the physical properties of these AgNPs has barely studied. Thus, in the present study, the biosynthetic potency of AgNPs from different fungal isolates as well as their antibacterial activity towards multidrug resistant bacteria, have been assessed. Aspergillus flavus and Penicillium crustosum have been selected for their higher reducing potency of silver ions (Ag+) into silver nanoparticles with strong physical stability, as revealed from UV, DLS and TEM analyses. The synthesized AgNPs by A. flavus have molecular size ranged from 5-10 nm with maximum UV-absorbance at λ384 nm, while that of P. crustosum was ranged from 30-40 nm and UV-absorbance at λ420 nm. The size and shape of AgNPs resolved from Zeta sizer, have been authenticated from the TEM analysis, having spherical to oval shape. The anti-bacterial activity of AgNPs was checked against the selected multi-drug resistant bacteria, Staphylococcus aureus and Escherichia coli. AgNPs from both fungal sources had an inhibitory effect for these bacteria isolates, however, AgNPs from A. flavus with molecular diameter 5 nm had a significant antibacterial effect "about 1.5 folds" higher than AgNPs of P. crustosum (size 35 nm). The MIC of A. flavus AgNPs was 0.42 and 0.41 µg/ml towards S. aureus and E. coli, while, the MIC of P. crustosum AgNPs was 0.86 µg/ml and 0.65 µg/ml, respectively. The bactericidal potency of AgNPs is strongly correlated to their molecular size. Thus, using of AgNPs (~5 nm)as solely antibacterial agent or in combination with commercial antibiotics to increase their potency and to reduce their overdosing and overusing, could be a novel platform for controlling the multi-drug resistance pathogenic bacteria.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
El-Sayed ASA,Ali Ddoi
10.4014/jmb.1806.05089subject
Has Abstractpub_date
2018-07-19 00:00:00eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1806.05089pub_type
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