Modulation of hydrolysis and transglycosylation activity of Thermus maltogenic amylase by combinatorial saturation mutagenesis.

abstract：:An efficient and simple fermentation process was developed for the production of gamma-amminobutyric acid (GABA) by Lactobacillus sakei B2-16. When the L. sakei B2-16 was cultivated in the rice bran extracts medium containing 4% sucrose, 1% yeast extract and 12% monosodium glutamate, the maximum GABA concentration rea...

journal_title：Journal of microbiology and biotechnology

authors： Kook MC,Seo MJ,Cheigh CI,Pyun YR,Cho SC,Park H

更新日期：2010-04-01 00:00:00

abstract：:Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosy...

journal_title：Journal of microbiology and biotechnology

authors： Pranawidjaja S,Choi SI,Lay BW,Kim P

更新日期：2015-06-01 00:00:00

abstract：:Quorum sensing is a process of cell-to-cell communication in which bacteria produce autoinducers as signaling molecules to sense cell density and coordinate gene expression. In the present study, a LuxI-type synthase, AnoI, and a LuxR-type regulator, AnoR, were identified in Acinetobacter nosocomialis, an important no...

journal_title：Journal of microbiology and biotechnology

authors： Oh MH,Choi CH

更新日期：2015-08-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:Rumex japonicus Houtt (RJH) is a valuable plant used in traditional medicine to treat several diseases, such as scabies and jaundice. In this study, Jurkat cell growth inhibitory extracts of R. japonicus roots were subjected to bioassay-guided fractionation, resulting in the isolation of three naphthalene derivatives ...

journal_title：Journal of microbiology and biotechnology

authors： Qiu Y,Li A,Lee J,Lee JE,Lee EW,Cho N,Yoo HM

更新日期：2020-12-28 00:00:00

abstract：:Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biol...

journal_title：Journal of microbiology and biotechnology

authors： Eom JS,Song J,Choi HS

更新日期：2015-04-01 00:00:00

abstract：:Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI d...

journal_title：Journal of microbiology and biotechnology

authors： Silveira ST,Gemelli S,Segalin J,Brandellli A

更新日期：2012-06-01 00:00:00

abstract：:In this study, a total of 58 different kinds of nuruk (a traditional Korean fermentation starter) were prepared, including 46 kinds of restored nuruk from ancient documents. Each nuruk was evaluated by analysis of its saccharification power, and the enzyme activities of glucoamylase, α-amylase, β-amylase, protease, an...

journal_title：Journal of microbiology and biotechnology

authors： Lee JE,Lee AR,Kim H,Lee E,Kim TW,Shin WC,Kim JH

更新日期：2017-05-28 00:00:00

abstract：:The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for th...

journal_title：Journal of microbiology and biotechnology

authors： Kim JY,Kim HK,Jang HJ,Kim EK,Kim MK

更新日期：2015-04-01 00:00:00

abstract：:Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CYPVdh, and CYPeryF, as templates. The lowest energy SPCYP147F1 model was then assessed for stereochemical quality and side-chain environment by Accelrys Discovery Studio 3.1 software. Further actives...

journal_title：Journal of microbiology and biotechnology

authors： Bhattarai S,Liou K,Oh TJ

更新日期：2012-07-01 00:00:00

abstract：:Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59-66% as analyzed by TLC and HPL...

journal_title：Journal of microbiology and biotechnology

authors： Ko JA,Ryu YB,Park JY,Kim CY,Kim JS,Nam SH,Lee WS,Kim YM

更新日期：2016-03-01 00:00:00

abstract：:Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their vi...

journal_title：Journal of microbiology and biotechnology

authors： Zhang J,Zhang X,Zhang L,Zhao Y,Niu C,Yang Z,Li S

更新日期：2014-02-28 00:00:00

abstract：:The objectives of this study were to determine the antibiotypes, SCCmec subtypes, PVL carriage, and genetic diversity of MRSA strains from a tertiary hospital. Sixtysix MRSA strains were selected randomly (2003, 2004, and 2007) and tested for the Panton-Valentine leukocidin gene, mecA gene, and SCCmec type via a PCR. ...

journal_title：Journal of microbiology and biotechnology

authors： Thong KL,Junnie J,Liew FY,Yusof MY,Hanifah YA

更新日期：2009-10-01 00:00:00

abstract：:Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to 85 degrees C. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We chara...

journal_title：Journal of microbiology and biotechnology

authors： Lim YR,Eun CY,Park HG,Han S,Han JS,Cho KS,Chun YJ,Kim D

更新日期：2010-03-01 00:00:00

abstract：:Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this st...

journal_title：Journal of microbiology and biotechnology

authors： Tang H,Hou J,Shen Y,Xu L,Yang H,Fang X,Bao X

更新日期：2013-11-28 00:00:00

abstract：:Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children wi...

journal_title：Journal of microbiology and biotechnology

authors： Wen H,Yin X,Yuan Z,Wang X,Su S

更新日期：2018-04-28 00:00:00

abstract：:Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms....

journal_title：Journal of microbiology and biotechnology

authors： Kim JE,Monmai C,Rod-In W,Jang AY,You SG,Lee SM,Park WJ

更新日期：2019-09-28 00:00:00

abstract：:Inactivation of Escherichia coli O157:H7 and Salmonella typhimurium was evaluated on inoculated chicken by aqueous chlorine dioxide (ClO2) treatment. Chicken samples were inoculated with 6-7 log CFU/g of Escherichia coli O157:H7 and Salmonella typhimurium, respectively. The chicken samples were then treated with 0, 50...

journal_title：Journal of microbiology and biotechnology

authors： Hong Y,Ku K,Kim M,Won M,Chung K,Song KB

更新日期：2008-04-01 00:00:00

abstract：:Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, ...

journal_title：Journal of microbiology and biotechnology

authors： Khalmuratova I,Choi DH,Woo JR,Jeong MJ,Oh Y,Kim YG,Lee IJ,Choo YS,Kim JG

更新日期：2020-11-28 00:00:00

abstract：:Polyester cloth (PC) was selected as a prospective inexpensive substitute separator material for microbial fuel cells (MFCs). PC was compared with a traditional Nafion proton exchange membrane (PEM) as an MFC separator by analyzing its physical and electrochemical properties. A single layer of PC showed higher mass tr...

journal_title：Journal of microbiology and biotechnology

authors： Kim T,Kang S,Sung JH,Kang YK,Kim YH,Jang JK

更新日期：2016-12-28 00:00:00

abstract：:The total methylotrophic population associated with rice plants from different cultivars was enumerated at three different stages: vegetative, flowering, and harvesting. The bacterial population in the leaf, rhizosphere soil, endophytic in the stem and roots, and epiphytic in the florets and grains were determined fro...

journal_title：Journal of microbiology and biotechnology

authors： Madhaiyan M,Poonguzhali S,Sa T

更新日期：2007-10-01 00:00:00

abstract：:Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a principal component of cigarette smoke. B[a]P can cause lung carcinogenesis and plays a key role in lung cancer progression. The role of B[a]P has been reported in lung cancer, but its effects on lung cancer stem cells (CSCs) have not been investigated. E...

journal_title：Journal of microbiology and biotechnology

authors： Bak Y,Jang HJ,Seo JH,No SH,Chae JI,Hong J,Yoon DY

更新日期：2018-03-28 00:00:00

abstract：:Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7- hydroxyl GA-7-O-β-D-glucoside (1) and ACDL3172-18-O-β-D-g...

journal_title：Journal of microbiology and biotechnology

authors： Huo Q,Li HM,Lee JK,Li J,Ma T,Zhang X,Dai Y,Hong YS,Wu CZ

更新日期：2016-01-01 00:00:00

abstract：:Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC1129...

journal_title：Journal of microbiology and biotechnology

authors： Koju D,Maharjan S,Dhakal D,Yoo JC,Sohng JK

更新日期：2012-08-01 00:00:00

abstract：:Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identificat...

journal_title：Journal of microbiology and biotechnology

authors： Chen C,Zhong G,Ren L,Lu C,Li Z,Wu Y

更新日期：2015-10-01 00:00:00

abstract：:Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobiliz...

journal_title：Journal of microbiology and biotechnology

authors： Yao J,Chen Q,Zhong G,Cao W,Yu A,Liu Y

更新日期：2014-01-01 00:00:00

abstract：:Dephosphocoenzyme A (CoaE) catalyzes the last step in the biosynthesis of the cofactor coenzyme A. In this study, we report the identification and application of CoaE from Stretomyces peucetius ATCC27952. After expression of coaE, the protein was found to have a molecular mass of 28.6 kDa. Purification of the His-tagg...

journal_title：Journal of microbiology and biotechnology

authors： Lee NR,Rimal H,Lee JH,Oh TJ

更新日期：2014-09-01 00:00:00

abstract：:In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbo...

journal_title：Journal of microbiology and biotechnology

authors： Lee EH,Cho KS,Kim J

更新日期：2010-09-01 00:00:00

abstract：:Metarhizium anisopliae is a widely studied model to understand the virulence factors that participate in pathogenicity. Proteases such as subtilisin-like enzymes (Pr1) and trypsin-like enzymes (Pr2) are considered important factors for insect cuticle degradation. In four M. anisopliae strains (798, 6342, 6345, and 634...

journal_title：Journal of microbiology and biotechnology

authors： Rosas-García NM,Avalos-de-León O,Villegas-Mendoza JM,Mireles-Martínez M,Barboza-Corona JE,Castañeda-Ramírez JC

更新日期：2014-11-28 00:00:00

abstract：:Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity ...

journal_title：Journal of microbiology and biotechnology

authors： Lin X,Liu S,Xie G,Chen J,Li P,Chen J

更新日期：2016-11-28 00:00:00