Abstract：
:Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this study, three β-glucosidases from different organisms were respectively expressed in Saccharomyces cerevisiae and the β-glucosidase from Saccharomycopsis fibuligera showed the best activity (5.2 U/ml). The recombinant strain with S. fibuligera β-glucosidase could metabolize cellobiose with a specific growth rate similar to the control strain in glucose. This recombinant strain showed higher hydrolysis efficiency in the cellulose simultaneous saccharification and fermentation, when using the Trichoderma reesei cellulase, which is short of the β-glucosidase activity. The final ethanol concentration was 110% (using Avicel) and 89% (using acid-pretreated corncob) higher than the control strain. These results demonstrated the effect of β-glucosidase secretion in the recombinant S. cerevisiae for enhancing cellulosic ethanol conversion.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Tang H,Hou J,Shen Y,Xu L,Yang H,Fang X,Bao Xdoi
10.4014/jmb.1305.05011subject
Has Abstractpub_date
2013-11-28 00:00:00pages
1577-85issue
11eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1305.05011journal_volume
23pub_type
杂志文章abstract：:The present study was aimed to investigate the effect of a probiotic, Enterococcus faecium, on the immune responses against infection with the marine fish pathogen Lactococcus garvieae in olive flounder (Paralichthys olivaceus). The immune responses were assessed by lysozyme activity, complement activity, protease act...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1108.08047
更新日期：2012-04-01 00:00:00
abstract：:Doenjang, a traditional Korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). Meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. To understand living bacterial community migration and the roles of bacteria in the manuf...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1401.01009
更新日期：2014-05-01 00:00:00
abstract：:In Caenorhabditis elegans, SHN-1 is the homologue of SHANK, a scaffolding protein. In this study, we determined the molecular basis for SHN-1/SHANK in the regulation of innate immune response to fungal infection. Mutation of shn-1 increased the susceptibility to Candida albicans infection and suppressed the innate imm...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.2006.06025
更新日期：2020-11-28 00:00:00
abstract：:In the continued search for inhibitors of enoyl-acyl carrier protein (ACP) reductase, we found that four acylbenzenediol sulfate metabolites from Streptomyces sp. AN1761 potently inhibited bacterial enoyl-ACP reductases of Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium tuberculosis. Their structure...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1209.09038
更新日期：2013-02-01 00:00:00
abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1001.01004
更新日期：2010-05-01 00:00:00
abstract：:Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：:Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinas...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2009-10-01 00:00:00
abstract：:Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:In the past, reactive oxygen species (ROS) have been considered a harmful byproduct of aerobic metabolism. However, accumulating evidence implicates redox homeostasis, which maintains appropriate ROS levels, in cell proliferation and differentiation in plants and animals. Similarly, ROS generation and signaling are in...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.1407.07072
更新日期：2014-11-28 00:00:00
abstract：:L-Arabinose, a five carbon sugar, has not been considered as an important bioresource because most studies have focused on D-xylose, another type of five-carbon sugar that is prevalent as a monomeric structure of hemicellulose. In fact, L-arabinose is also an important monomer of hemicellulose, but its content is much...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
doi：10.4014/jmb.1812.12015
更新日期：2019-03-28 00:00:00
abstract：:The adhesion of Campylobacter jejuni to chicken skin, along with the associated morphological changes under aerobic conditions at 4, 25, and 37 degrees C and microaerobic (O2 5%, CO2 10%, N2 85%) conditions, were investigated using confocal laser scanning microscopy (CLSM), flow cytometry, and plate counting. The morp...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-02-01 00:00:00
abstract：:The dye decolorization rate in a cell-free culture broth of the white-rot fungus Trametes versicolor CBR43 was studied, including the effects of inhibitors of NaCl, Zn(II), and Cd(II) on dye decolorization activity. The maximum rates of dye decolorization in cell-free culture broth were 1,410, 44.7, 41.2, and 0.19 μmo...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1608.08008
更新日期：2017-01-28 00:00:00
abstract：:This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1306.06036
更新日期：2014-06-28 00:00:00
abstract：:Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
abstract：:Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.2006.06050
更新日期：2020-11-28 00:00:00
abstract：:Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0903.3027
更新日期：2009-11-01 00:00:00
abstract：:As a provirus, polydnavirus has a segmented DNA genome on chromosome(s) of host wasp. It contains several genes in each segment that presumably play critical roles in regulating physiological processes of target insect parasitized by the wasp. A cysteine-rich protein 1 (CRP1) is present in the polydnavirus Cotesia plu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0808.495
更新日期：2009-06-01 00:00:00
abstract：:The correlation between alcoholic fermentation rate, measured as carbon dioxide (CO2) evolution, and the rate of hydrogen sulfide (H2S) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace H2S were directly impacted by yeast assimilable nitrogenous ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1010.10058
更新日期：2011-05-01 00:00:00
abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0802.130
更新日期：2009-02-01 00:00:00
abstract：:Salmonellosis is a form of gastroenteritis caused by Salmonella infection. The main transmission route of salmonellosis has been identified as poorly cooked meat and poultry products contaminated with Salmonella. However, in recent years, the number of outbreaks attributed to contaminated raw produce has increased dra...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.2007.07036
更新日期：2020-11-28 00:00:00
abstract：:Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1405.05043
更新日期：2014-09-01 00:00:00
abstract：:A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell g...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-12-01 00:00:00
abstract：:Proteomic analysis led to identification of the proteins of Vibrio vulnificus that were induced upon exposure to INT-407 cells, and 7 of which belong to the functional categories such as amino acid transport/metabolism, nucleotide transport/metabolism, posttranslational modification/protein turnover/chaperones, and tr...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-05-01 00:00:00
abstract：:A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2010-12-01 00:00:00
abstract：:Accumulating evidence indicates that lactic acid bacteria could improve host physiology and lipid metabolism. To investigate the effect of the gut microbiota on host lipid metabolism, a hyperlipidemic rat model was established by feeding rats a high-fat diet for 28 days, and the gut microbiota of the rats was analyzed...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1409.09085
更新日期：2015-05-01 00:00:00
abstract：:A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1411.11012
更新日期：2015-06-01 00:00:00
abstract：:Multidomain proteins for the biochemical analysis of the scouring efficiency of cotton fabrics were constructed by the fusion of a reporter moiety in the N-terminal and the cellulose binding domain (CBD) in the C-terminal. Based on the specific binding of the CBD of Cellulomonas fimi exoglucanase (Cex) to crystalline ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-03-01 00:00:00
abstract：:The dependence of the catalytic properties of horseradish peroxidase on the structural changes of ionic liquids was investigated with two water-miscible ionic liquids, N-butyl-3- methypyridinium tetrafluoroborate ([BMPy][BF4]) and 1-butyl- 3-methylimidazolium methylsulfate ([BMIM][MeSO4]), each of which shares an anio...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2009-07-01 00:00:00
abstract：:HpaGXooc, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the HpaGXooc protein in Bacillus subtilis, ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0802.154
更新日期：2009-02-01 00:00:00
