Abstract：
:Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capability with minimal collagen damage, indicating promising potential applications in the leather industry. In this study, the strain's extracellular proteome was identified by LC-MS/MS-based shotgun proteomic analysis, and their related secretory pathways were characterized by BLAST searches. A total of 513 proteins, including 100 actual secreted and 413 intracellular proteins, were detected in the extracellular proteome. The functions of these secreted proteins were elucidated and four complete secretory systems (Sec, Tat, Com, and ABC transporter) were proposed for B. pumilus. These data provide B. pumilus a comprehensive extracellular proteome profile, which is a valuable theoretical and applicative basis for future genetic modifications and development of industrial enzymes.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Wang C,Yu S,Song T,He T,Shao H,Wang Hdoi
10.4014/jmb.1602.02042subject
Has Abstractpub_date
2016-11-28 00:00:00pages
1993-2005issue
11eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1602.02042journal_volume
26pub_type
杂志文章abstract：:Marine sediments are a microbial biosphere with an unknown physiology, and the sediments harbor numerous distinct phylogenetic lineages of Bacteria and Archaea that are at present uncultured. In this study, the structure of the archaeal and bacterial communities was investigated in the surface and subsurface sediments...
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abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...
journal_title：Journal of microbiology and biotechnology
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pub_type： 杂志文章,评审
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1512.12056
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更新日期：2009-07-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2011-03-01 00:00:00
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更新日期：2008-12-01 00:00:00
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更新日期：2009-10-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-10-01 00:00:00
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更新日期：2008-10-01 00:00:00
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