Quantitative real-time PCR assay for detection of Paenibacillus polymyxa using membrane-fusion protein-based primers.

Abstract:

:Paenibacillus polymyxa is known to be a plant-growthpromoting rhizobacterium. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection and quantitation of P. polymyxa using a primer pair based on the sequence of a membranefusion protein for the amplification of a 268 bp DNA fragment. This study reports that the qPCR-based method is applicable for the rapid and sensitive detection of P. polymyxa and can be used as an alternative method for agricultural soil monitoring.

journal_name

J Microbiol Biotechnol

authors

Cho MS,Park DS,Lee JW,Chi HY,Sohn SI,Jeon BK,Ma JB

doi

10.4014/jmb.1207.07018

subject

Has Abstract

pub_date

2012-11-01 00:00:00

pages

1575-9

issue

11

eissn

1017-7825

issn

1738-8872

pii

JMB022-11-17

journal_volume

22

pub_type

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