Fungal diversity in composting process of pig manure and mushroom cultural waste based on partial sequence of large subunit rRNA.

Abstract:

:Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU) ribosomal RNA (rRNA) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; Geotrichum sp., Debaryomyces hansenii, Monographella nivalis, Acremonium strictum, Acremonium alternatum, Cladosporium sphaerospermum, Myriangium durosai, Pleurotus eryngii, Malassezia globosa, Malassezia restricta, Rhodotorula glutinis, and Fusarium sporotrichioides. Geotrichum sp. of the class Saccharomycetes was the most predominant fungal species throughout the composting process (185 out of a total of 236 identified clones, or 78.4%), followed by Acremonium strictum (7.6%), Monographella nivalis (5.1%), and Pleurotus eryngii (3.8%). The prevalence of Geotrichum sp. was the lowest (61.1%) at the beginning of composting, and then gradually increased to 92.5% after 10 days of composting.

journal_name

J Microbiol Biotechnol

authors

Cho KM,Kwon EJ,Kim SK,Kambiranda DM,Math RK,Lee YH,Kim J,Yun HD,Kim H

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

743-8

issue

8

eissn

1017-7825

issn

1738-8872

pii

8331

journal_volume

19

pub_type

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