Production of alkaline protease by entrapped Bacillus licheniformis cells in repeated batch process.

abstract：:A highly sensitive and specific TaqMan real-time PCR was used to quantify hepatopancreatic parvovirus (HPV) type III infections in wild broodstocks and hatchery-reared postlarvae (PL) of Fenneropenaeus chinensis. Totals of 159 and 162 wild brooders from three locations were captured, and 140 and 180 PL were obtained f...

journal_title：Journal of microbiology and biotechnology

authors： Jang IK,Suriakala K,Kim JS,Meng XH,Choi TJ

更新日期：2011-11-01 00:00:00

abstract：:With emphasis on thermal behavior in presence of different pH conditions and salts, the kinetic and thermodynamic parameters of purified polygalacturonase (PG) of E. carotovora subsp. carotovora (Ecc) BR1 were studied since characterization of an enzyme is significant in the context of burgeoning biotechnological appl...

journal_title：Journal of microbiology and biotechnology

authors： Maisuria VB,Patel VA,Nerurkar AS

更新日期：2010-07-01 00:00:00

abstract：:The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups o...

journal_title：Journal of microbiology and biotechnology

authors： Hong H,Jo JR,Yeon JH,Hong JT,Jung KH,Yoo SK,Jang BC

更新日期：2011-02-01 00:00:00

abstract：:Drought stress is threatening the growth and productivity of many economical crops. Therefore, it is necessary to establish innovative and efficient approaches for improving crop growth and productivity. Here we investigated the potentials of the cell-free extract of Actinobacteria (Ac) isolated from a semi-arid habit...

journal_title：Journal of microbiology and biotechnology

authors： Warrad M,Hassan YM,Mohamed MSM,Hagagy N,Al-Maghrabi OA,Selim S,Saleh AM,AbdElgawad H

更新日期：2020-08-28 00:00:00

abstract：:Hrr25, a casein kinase 1 δ/ε homolog in budding yeast, is essential to set up mono-orientation of sister kinetochores during meiosis. Hrr25 kinase activity coordinates sister chromatid cohesion via cohesin phosphorylation. Here, we investigated the prophase role of Hrr25 using the auxin-inducible degron system and by ...

journal_title：Journal of microbiology and biotechnology

authors： Lee MS,Joo JH,Kim K

更新日期：2017-06-28 00:00:00

abstract：:The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protectio...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim Y

更新日期：2020-07-28 00:00:00

abstract：:Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both...

journal_title：Journal of microbiology and biotechnology

authors： Sun YT,Zhao L,Ye ZY,Fan L,Liu XP,Tan WS

更新日期：2013-11-28 00:00:00

abstract：:The fungal strain EML-DML3PNa1 isolated from leaf of white dogwood (Cornus alba L.) showed strong nematicidal activity with juvenile mortality of 87.6% at a concentration of 20% fermentation broth filtrate at 3 days after treatment. The active fungal strain was identified as Aspergillus oryzae, which belongs to sectio...

journal_title：Journal of microbiology and biotechnology

authors： Kim TY,Jang JY,Jeon SJ,Lee HW,Bae CH,Yeo JH,Lee HB,Kim IS,Park HW,Kim JC

更新日期：2016-08-28 00:00:00

abstract：:Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The ...

journal_title：Journal of microbiology and biotechnology

authors： Hong I,Kim YS,Choi SG

更新日期：2010-02-01 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after t...

journal_title：Journal of microbiology and biotechnology

authors： Jeong SJ,Park JY,Lee JY,Lee KW,Cho KM,Kim GM,Shin JH,Kim JS,Kim JH

更新日期：2015-11-01 00:00:00

abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...

journal_title：Journal of microbiology and biotechnology

authors： Zheng CJ,Sohn MJ,Chi SW,Kim WG

更新日期：2010-05-01 00:00:00

abstract：:Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. c...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Kim Y,Yoon SH

更新日期：2019-03-28 00:00:00

abstract：:Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined...

journal_title：Journal of microbiology and biotechnology

authors： Kim HJ,Kim YH,Shin JH,Bhatia SK,Sathiyanarayanan G,Seo HM,Choi KY,Yang YH,Park K

更新日期：2015-07-01 00:00:00

abstract：:Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to...

journal_title：Journal of microbiology and biotechnology

authors： Dumorné K,Córdova DC,Astorga-Eló M,Renganathan P

更新日期：2017-04-28 00:00:00

abstract：:Osteoarthritis (OA) is the most common rheumatic pathology. One of the major objectives of OA research is the development of early diagnostic strategies such as those using proteomic technology. Synovial fluid (SF) in OA patients is a potential source of biomarkers for OA. The efficient and reliable preparation of SF ...

journal_title：Journal of microbiology and biotechnology

authors： Kong MK,Min BH,Lee PC

更新日期：2012-05-01 00:00:00

abstract：:Marine sediments are a microbial biosphere with an unknown physiology, and the sediments harbor numerous distinct phylogenetic lineages of Bacteria and Archaea that are at present uncultured. In this study, the structure of the archaeal and bacterial communities was investigated in the surface and subsurface sediments...

journal_title：Journal of microbiology and biotechnology

authors： Choi H,Koh HW,Kim H,Chae JC,Park SJ

更新日期：2016-05-28 00:00:00

abstract：:Three different polysaccharide-peptide complexes (PPC, named as Fr-I, Fr-II, and Fr-III) were produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala, and their anticancer activities were investigated in human hepatocarcinoma (HepG2) and neuroblastoma (SK-N-SH) cells. The highest i...

journal_title：Journal of microbiology and biotechnology

authors： Oh JY,Baek YM,Kim SW,Hwang HJ,Hwang HS,Lee SH,Yun JW

更新日期：2008-03-01 00:00:00

abstract：:Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...

journal_title：Journal of microbiology and biotechnology

authors： Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh C

更新日期：2014-11-28 00:00:00

abstract：:An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protei...

journal_title：Journal of microbiology and biotechnology

authors： Zhao S,Zhao Y,Li S,Zhang G,Wang H,Ng TB

更新日期：2010-04-01 00:00:00

abstract：:Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, ...

journal_title：Journal of microbiology and biotechnology

authors： Khalmuratova I,Choi DH,Woo JR,Jeong MJ,Oh Y,Kim YG,Lee IJ,Choo YS,Kim JG

更新日期：2020-11-28 00:00:00

abstract：:In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbo...

journal_title：Journal of microbiology and biotechnology

authors： Lee EH,Cho KS,Kim J

更新日期：2010-09-01 00:00:00

abstract：:Based on its α-amylase activity at pH 5.0 and optimal pH of the crude enzyme, a strain (named B-5) with acid α-amylase production was screened. The B-5 strain was identified as Bacillus amyloliquefaciens through morphological, physiological, and biochemical characteristics analysis, as well as 16S rDNA phylogenetic an...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Jia W,An Y,Cheng K,Wang M,Yang S,Chen H

更新日期：2015-06-01 00:00:00

abstract：:Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total ...

journal_title：Journal of microbiology and biotechnology

authors： Siddiquee S,Tan SG,Yusof UK

更新日期：2010-09-01 00:00:00

abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...

journal_title：Journal of microbiology and biotechnology

authors： Wang J,Wang ZY,He XP,Zhang BR

更新日期：2010-11-01 00:00:00

abstract：:A beta-1,3-1,4-glucanase from the fungus Laetiporus sulphureus var. miniatus was purified as a single 26 kDa band by ammonium sulfate precipitation, HiTrap Q HP, and UNO Q ion-exchange chromatography, with a specific activity of 29 U/mg. The molecular mass of the native enzyme was 52 kDa as a dimer by gel filtration. ...

journal_title：Journal of microbiology and biotechnology

authors： Hong MR,Kim YS,Joo AR,Lee JK,Kim YS,Oh DK

更新日期：2009-08-01 00:00:00

abstract：:Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...

journal_title：Journal of microbiology and biotechnology

authors： Park IH,Kim SH,Lee YS,Lee SC,Zhou Y,Kim CM,Ahn SC,Choi YL

更新日期：2009-02-01 00:00:00

abstract：:Tetanus is a specific infectious disease, which is often associated with catastrophic events such as earthquakes, traumas, and war wounds. The obligate anaerobe Clostridium tetani is the pathogen that causes tetanus. Once the infection of tetanus progresses to an advanced stage within the wounds of limbs, the rates of...

journal_title：Journal of microbiology and biotechnology

authors： Jiang D,Pu X,Wu J,Li M,Liu P

更新日期：2013-01-01 00:00:00

abstract：:Retrovirus tropism can be restricted by host cell factors such as Fv1, TRIM5alpha, and Lv1 that inhibit infection by targeting the incoming viral capsid. The Fv1 gene inhibits murine leukemia virus infection in mice, but the precise mechanism of Fv1-mediated restriction is poorly understood. Our previous studies had d...

journal_title：Journal of microbiology and biotechnology

authors： Bae EH,Park SH,Park SM,Park JW,Lim MS,Jung YT

更新日期：2008-04-01 00:00:00

abstract：:Ingredients of soy and fermented soy products have been widely utilized as food supplements for health-enhancing properties. The aim of this study was to evaluate the effects of fermented soymilk (FSM) and soymilk (SM) on free fatty acid-induced lipogenesis in the hepatocellular steatosis model. HepG2 cells were incub...

journal_title：Journal of microbiology and biotechnology

authors： Ahn SB,Wu WH,Lee JH,Jun DW,Kim J,Kim R,Lee TB,Jun JH

更新日期：2018-02-28 00:00:00