Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration.

abstract：:Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of ...

journal_title：Journal of microbiology and biotechnology

authors： Gao F,Li M,Liu W,Bai Y,Tu T,Wang Y,Zhang J,Luo H,Yao B,Huang H,Su X

更新日期：2020-02-28 00:00:00

abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...

journal_title：Journal of microbiology and biotechnology

authors： Long D,Tang X,Cai K,Chen G,Shen C,Shi J,Chen L,Chen Y

更新日期：2013-08-01 00:00:00

abstract：:Glucose dehydrogenase (GDH) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. In this study, the glucose 1-dehydrogenase B gene (gdhB) was cloned from Bacillus thuringiensis subsp. kurstaki, and wild-type (GDH-BTWT) and His-tagged (GDH-BTN-...

journal_title：Journal of microbiology and biotechnology

authors： Hyun J,Abigail M,Choo JW,Ryu J,Kim HK

更新日期：2016-10-28 00:00:00

abstract：:Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×10(7) CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×...

journal_title：Journal of microbiology and biotechnology

authors： Wang MH,Peng P,Liu YM,Jia RB,Li L

更新日期：2013-02-01 00:00:00

abstract：:An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50°C. The enzyme was stable between pH 6.0 and 11.0 at 25°C, 40°C, and 50°C for 24 h. The Km and Vmax of the enzy...

journal_title：Journal of microbiology and biotechnology

authors： Bakir ZB,Metin K

更新日期：2016-06-28 00:00:00

abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...

journal_title：Journal of microbiology and biotechnology

authors： Zheng CJ,Sohn MJ,Chi SW,Kim WG

更新日期：2010-05-01 00:00:00

abstract：:The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predomin...

journal_title：Journal of microbiology and biotechnology

authors： Zhao W,Zhang J,Jiang YY,Zhao X,Hao XN,Li L,Yang ZN

更新日期：2018-08-28 00:00:00

abstract：:Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

journal_title：Journal of microbiology and biotechnology

authors： Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

更新日期：2013-04-01 00:00:00

abstract：:Trimethylamine (TMA) is a well-known off-odor compound in fish and fishery products and is a metabolic product of trimethylamine N-oxide (TMAO) generated by the enzymatic action of microorganisms. The off-odor is a factor that can debase the value of fish and fishery products. The present study aimed to remove TMA usi...

journal_title：Journal of microbiology and biotechnology

authors： Park SK,Jo DM,Yu D,Khan F,Lee YB,Kim YM

更新日期：2020-10-28 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the r...

journal_title：Journal of microbiology and biotechnology

authors： Kim DG,Ahn SH,Kim LH,Park KJ,Hong YK,Kong IS

更新日期：2008-11-01 00:00:00

abstract：:Mycophenolic acid (MPA) is an antibiotic produced by Penicillium brevicompactum. MPA has antifungal, antineoplastic, and immunosuppressive functions, among others. β-Hydroxy-β-methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the bypass metabolic pathway. The inhibitory activity of HMG-CoA lyase increases the MPA ...

journal_title：Journal of microbiology and biotechnology

authors： Dong Y,Zhang J,Xu R,Lv X,Wang L,Sun A,Wei D

更新日期：2016-11-28 00:00:00

abstract：:Scientific research regarding the use of live bacterial cells for therapeutic purposes has been rapidly growing over the years and has generated considerable interest to scientists and health professionals. Probiotics are defined as essential live microorganisms which, when administered in adequate amounts, confer a h...

journal_title：Journal of microbiology and biotechnology

authors： Islam MA,Yun CH,Choi YJ,Cho CS

更新日期：2010-10-01 00:00:00

abstract：:Clostridium difficile causes mucosal damage and diarrhea by releasing two exotoxins: toxin A and toxin B. C. difficile colitis is associated with alterations in bowel flora and the failure to mount an effective antibody response. The aim of the current study was to investigate whether antitoxin sera prevent toxin-A-in...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Nam HJ,Kang JK,Seok H,Hwang JS,Kim H

更新日期：2014-05-01 00:00:00

abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Qian H,Zhang WG

更新日期：2010-01-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The alpha-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a...

journal_title：Journal of microbiology and biotechnology

authors： Simila J,Gernig A,Murray P,Fernandes S,Tuohy MG

更新日期：2010-12-01 00:00:00

abstract：:Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi. Taxol extracted from...

journal_title：Journal of microbiology and biotechnology

authors： Garyali S,Kumar A,Reddy MS

更新日期：2013-10-28 00:00:00

abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...

journal_title：Journal of microbiology and biotechnology

authors： Park HJ,Min J,Ahn JM,Cho SJ,Ahn JY,Kim YH

更新日期：2015-02-01 00:00:00

abstract：:A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced i...

journal_title：Journal of microbiology and biotechnology

authors： Khang YH,Park HY,Jeong YS,Kim JA,Kim YH

更新日期：2009-05-01 00:00:00

abstract：:Leaves are the major biomass-producing organs in herbaceous plants and mainly develop during vegetative stage by activities of shoot apical meristem. There is a strong correlation between leaf number and bolting, a characteristic phenotype during the transition to reproductive phase in Arabidopsis thaliana. In order t...

journal_title：Journal of microbiology and biotechnology

authors： Lee BH

更新日期：2009-06-01 00:00:00

abstract：:TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic alpha- helical barrel domain and a membrane-embedded beta-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and dr...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Yum S,Jo WS,Lee BL,Jeong MH,Ha NC

更新日期：2008-05-01 00:00:00

abstract：:Tuberculosis, which is caused by Mycobacterium tuberculosis (Mtb), is among the most pressing worldwide problems. Mtb uniquely interacts with innate immune cells through various pattern recognition receptors. These interactions initiate several inflammatory pathways that play essential roles in controlling Mtb pathoge...

journal_title：Journal of microbiology and biotechnology

authors： Kim JS,Kim YR,Yang CS

更新日期：2019-10-28 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:A novel psychrotolerant Psychrobacillus strain PB01, isolated from an Antarctic iceberg, was comparatively analyzed with five related strains. The complete genome of strain PB01 consists of a single circular chromosome (4.3 Mb) and a plasmid (19 Kb). As potential low-temperature adaptation strategies, strain PB01 has ...

journal_title：Journal of microbiology and biotechnology

authors： Choi JY,Kim SC,Lee PC

更新日期：2020-02-28 00:00:00

abstract：:The aim of this work is to investigate the protective efficacy of emodin, an active, naturally-occurring anthraquinone derivative of several traditional Chinese herbs, against Brucella abortus infection in macrophages. Brucella were incubated with different concentrations of emodin and showed that bacterial survival r...

journal_title：Journal of microbiology and biotechnology

authors： Huy TXN,Reyes AWB,Hop HT,Arayan LT,Son VH,Min W,Lee HJ,Kim S

更新日期：2018-10-28 00:00:00

abstract：:To investigate the potential applications of bacterial heme, aminolevulinic acid synthase (HemA) was expressed in a Corynebacterium glutamicum HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 4...

journal_title：Journal of microbiology and biotechnology

authors： Choi SI,Park J,Kim P

更新日期：2017-03-28 00:00:00

abstract：:The purified endochitosanase (Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers (GlcN)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this e...

journal_title：Journal of microbiology and biotechnology

authors： Gao XA,Jung WJ,Kuk JH,Park RD

更新日期：2009-04-01 00:00:00

abstract：:The correlation between alcoholic fermentation rate, measured as carbon dioxide (CO2) evolution, and the rate of hydrogen sulfide (H2S) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace H2S were directly impacted by yeast assimilable nitrogenous ...

journal_title：Journal of microbiology and biotechnology

authors： Butzke CE,Park SK

更新日期：2011-05-01 00:00:00

abstract：:Ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. To use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysi...

journal_title：Journal of microbiology and biotechnology

authors： Souza AC,Carvalho FP,Silva e Batista CF,Schwan RF,Dias DR

更新日期：2013-10-28 00:00:00

abstract：:Aging is associated with distinct changes in immune cells and a decline in immune function, leading to increased susceptibility to infection and reduced responses to vaccination. Certain strains of lactic acid bacteria exert beneficial effects on the immune system. Previously, we reported that Weissella cibaria JW15 i...

journal_title：Journal of microbiology and biotechnology

authors： Park HE,Kang KW,Kim BS,Lee SM,Lee WK

更新日期：2017-12-28 00:00:00