Abstract：
:A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced in a 500-l fermentor were likely to be stable in the range of pH 5 to 8 and 0 degree to 40 degrees . Their adhesive property enabled an easy and rapid immobilization of enzymes or antibodies on solid materials such as plastics, glass, sol-gel films, and intestinal epithelial cells. Owing to their affinity towards intestinal cells and immunoglobulin G, the Slayer fusion proteins enabled the adhesion of antibodies to human epithelial cells. In addition, feeding a mixture of the S-layer fusion proteins and antibodies against neonatal calf diarrhea (coronavirus, rotavirus, Escherichia coli, and Salmonella typhimurium) to Hanwoo calves resulted in 100% prevention of neonatal calf diarrhea syndrome (p<0.01),whereas feeding antibodies only resulted in 56% prevention.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Khang YH,Park HY,Jeong YS,Kim JA,Kim YHdoi
10.4014/jmb.0805.325subject
Has Abstractpub_date
2009-05-01 00:00:00pages
511-9issue
5eissn
1017-7825issn
1738-8872pii
8277journal_volume
19pub_type
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更新日期：2009-04-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-08-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2012-02-01 00:00:00
