Lipoteichoic Acid Isolated from Staphylococcus aureus Induces Both Epithelial-Mesenchymal Transition and Wound Healing in HaCaT Cells.

Abstract:

:Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There are many structural and functional varieties of LTA, which vary according to the different species of gram-positive bacteria that produce them. In this study, we examined whether LTA isolated from Staphylococcus aureus (aLTA) affects the expression of junction proteins in keratinocytes. In HaCaT cells, tight junction-related gene expression was not affected by aLTA, whereas adherens junction-related gene expression was modified. High doses of aLTA induced the phosphorylation of extracellular signal-regulated protein kinases 1 and 2, which in turn induced the epithelial-mesenchymal transition (EMT) of HaCaT cells. When cells were given a low dose of aLTA, however, NF-κB was activated and the total cell population increased. Taken together, our study suggests that LTA from S. aureus infections in the skin may contribute both to the outbreak of EMT-mediated carcinogenesis and to the genesis of wound healing in a dose-dependent manner.

journal_name

J Microbiol Biotechnol

authors

Kim S,Kim HE,Kang B,Lee YW,Kim H,Chung DK

doi

10.4014/jmb.1703.03068

subject

Has Abstract

pub_date

2017-10-28 00:00:00

pages

1820-1826

issue

10

eissn

1017-7825

issn

1738-8872

pii

10.4014/jmb.1703.03068

journal_volume

27

pub_type

杂志文章
  • Effects of silkworm hemolymph on cell viability and hCTLA4Ig production in transgenic rice cell suspension cultures.

    abstract::Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Cheon SH,Lee KH,Kwon JY,Ryu HN,Yu DH,Choi YS,Kim DI

    更新日期:2007-12-01 00:00:00

  • Toxicity evaluation of complex metal mixtures using reduced metal concentrations: application to iron oxidation by Acidithiobacillus ferrooxidans.

    abstract::In this study, we investigated the inhibition effects of single and mixed heavy metal ions (Zn2+, Ni2+, Cu2+, and Cd2+) on iron oxidation by Acidithiobacillus ferrooxidans. Effects of metals on the iron oxidation activity of A. ferrooxidans are categorized into four types of patterns according to its oxidation behavio...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Cho KS,Ryu HW,Choi HM

    更新日期:2008-07-01 00:00:00

  • Programmed Cell Death in Bacterial Community: Mechanisms of Action, Causes and Consequences.

    abstract::In the bacterial community, unicellular organisms act together as a multicellular being. Bacteria interact within the community and programmed cell death (PCD) in prokaryotes is a sort of altruistic action that enables the whole population to thrive. Genetically, encoded cell death pathways are triggered by DNA damage...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1904.04017

    authors: Lee H,Lee DG

    更新日期:2019-07-28 00:00:00

  • Characterization of a recombinant thermostable xylanase from hot spring thermophilic Geobacillus sp. TC-W7.

    abstract::A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at 75 de...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1203.03045

    authors: Liu B,Zhang N,Zhao C,Lin B,Xie L,Huang Y

    更新日期:2012-10-01 00:00:00

  • Purification and Characterization of the Laccase Involved in Dye Decolorization by the White-Rot Fungus Marasmius scorodonius.

    abstract::Marasmius scorodonius secretes an extracellular laccase in potato dextrose broth, and this enzyme was purified up to 206-fold using (NH4)2SO4 precipitation and a Hi-trap Q Sepharose column. The molecular mass of the purified laccase was estimated to be ~67 kDa by SDS-PAGE. The UV/vis spectrum of the enzyme was nontypi...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1701.01004

    authors: Jeon SJ,Lim SJ

    更新日期:2017-06-28 00:00:00

  • Bioconversion of acrylonitrile to acrylic acid by rhodococcus ruber strain AKSH-84.

    abstract::A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was optimized for qua...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1006.06044

    authors: Kamal A,Kumar MS,Kumar CG,Shaik T

    更新日期:2011-01-01 00:00:00

  • TiO₂ Nanoparticles from Baker's Yeast: A Potent Antimicrobial.

    abstract::Titanium dioxide (TiO2) has wide applications in food, cosmetics, pharmaceuticals and manufacturing due to its many properties such as photocatalytic activity and stability. In this study, the biosynthesis of TiO2 nanoparticles (NPs) was achieved by using Baker's yeast. TiO2 NPs were characterized by X-ray Diffraction...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1807.07005

    authors: Peiris M,Gunasekara T,Jayaweera PM,Fernando S

    更新日期:2018-10-28 00:00:00

  • In Vitro Antiviral Activity of Cinnamomum cassia and Its Nanoparticles Against H7N3 Influenza A Virus.

    abstract::Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other met...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1508.08024

    authors: Fatima M,Zaidi NU,Amraiz D,Afzal F

    更新日期:2016-01-01 00:00:00

  • Sequence analysis of a cryptic plasmid pKW2124 from Weissella cibaria KLC140 and construction of a surface display vector.

    abstract::Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1301.01018

    authors: Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

    更新日期:2013-04-01 00:00:00

  • An investigation into the relationship between metabolic responses and energy regulation in antibody-producing cell.

    abstract::Energy-efficient metabolic responses were often noted in high-productive cultures. To better understand these metabolic responses, an investigation into the relationship between metabolic responses and energy regulation was conducted via a comparative analysis among cultures with different energy source supplies. Both...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1307.07019

    authors: Sun YT,Zhao L,Ye ZY,Fan L,Liu XP,Tan WS

    更新日期:2013-11-28 00:00:00

  • Improving Catalytic Efficiency and Changing Substrate Spectrum for Asymmetric Biocatalytic Reductive Amination.

    abstract::With the advantages of biocatalytic method, enzymes have been excavated for the synthesis of chiral amino acids by the reductive amination of ketones, offering a promising way of producing pharmaceutical intermediates. In this work, a robust phenylalanine dehydrogenase (PheDH) with wide substrate spectrum and high cat...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1907.07015

    authors: Jiang W,Wang Y

    更新日期:2020-01-28 00:00:00

  • Synthetic Bacteria for Therapeutics.

    abstract::Synthetic biology builds programmed biological systems for a wide range of purposes such as improving human health, remedying the environment, and boosting the production of valuable chemical substances. In recent years, the rapid development of synthetic biology has enabled synthetic bacterium-based diagnoses and the...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1904.04016

    authors: Vo P,Lee HM,Na D

    更新日期:2019-06-28 00:00:00

  • Restoration of Traditional Korean Nuruk and Analysis of the Brewing Characteristics.

    abstract::In this study, a total of 58 different kinds of nuruk (a traditional Korean fermentation starter) were prepared, including 46 kinds of restored nuruk from ancient documents. Each nuruk was evaluated by analysis of its saccharification power, and the enzyme activities of glucoamylase, α-amylase, β-amylase, protease, an...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1610.10039

    authors: Lee JE,Lee AR,Kim H,Lee E,Kim TW,Shin WC,Kim JH

    更新日期:2017-05-28 00:00:00

  • Taxol production by an endophytic fungus, Fusarium redolens, isolated from Himalayan yew.

    abstract::Different endophytic fungi isolated from Himalayan Yew plants were tested for their ability to produce taxol. The BAPT gene (C-13 phenylpropanoid side chain-CoA acetyl transferase) involved in the taxol biosynthetic pathway was used as a molecular marker to screen taxol-producing endophytic fungi. Taxol extracted from...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1305.05070

    authors: Garyali S,Kumar A,Reddy MS

    更新日期:2013-10-28 00:00:00

  • Surface-Displayed Porcine IFN-λ3 in Lactobacillus plantarum Inhibits Porcine Enteric Coronavirus Infection of Porcine Intestinal Epithelial Cells.

    abstract::Interferon (IFN)-λ plays an essential role in mucosal cells which exhibit strong antiviral activity. Lactobacillus plantarum (L. plantarum) has substantial application potential in the food and medical industries because of its probiotic properties. Alphacoronaviruses, especially porcine epidemic diarrhea virus (PEDV)...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1909.09041

    authors: Liu YS,Liu Q,Jiang YL,Yang WT,Huang HB,Shi CW,Yang GL,Wang CF

    更新日期:2020-04-28 00:00:00

  • Heme Derived from Corynebacterium glutamicum: A Potential Iron Additive for Swine and an Electron Carrier Additive for Lactic Acid Bacterial Culture.

    abstract::To investigate the potential applications of bacterial heme, aminolevulinic acid synthase (HemA) was expressed in a Corynebacterium glutamicum HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 4...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1611.11010

    authors: Choi SI,Park J,Kim P

    更新日期:2017-03-28 00:00:00

  • Cloning, Overexpression, and Characterization of a Metagenome-Derived Phytase with Optimal Activity at Low pH.

    abstract::A phytase gene was identified in a publicly available metagenome derived from subsurface groundwater, which was deduced to encode for a protein of the histidine acid phosphatase (HAP) family. The nucleotide sequence of the phytase gene was chemically synthesized and cloned, in order to further overexpress the phytase ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1411.11012

    authors: Tan H,Wu X,Xie L,Huang Z,Gan B,Peng W

    更新日期:2015-06-01 00:00:00

  • Optimal Temperature and Light Intensity for Improved Mixotrophic Metabolism of Chlorella sorokiniana Treating Livestock Wastewater.

    abstract::Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experi...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1707.07007

    authors: Lee TH,Jang JK,Kim HW

    更新日期:2017-11-28 00:00:00

  • Change of sludge consortium in response to sequential adaptation to benzene, toluene, and o-xylene.

    abstract::Activated sludge was sequentially adapted to benzene, toluene, and o-xylene (BTX) to study the effects on the change of microbial community. Sludge adapted to BTX separately degraded each by various rates in the following order; toluene>o-xylene>benzene. Degradation rates were increased after exposure to repeated spik...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Park JY,Sang BI

    更新日期:2007-11-01 00:00:00

  • Evaluation of macroporous and microporous carriers for CHO-K1 cell growth and monoclonal antibody production.

    abstract::The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monocl...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1304.04011

    authors: Rodrigues ME,Costa AR,Fernandes P,Henriques M,Cunnah P,Melton DW,Azeredo J,Oliveira R

    更新日期:2013-09-28 00:00:00

  • Pinus Densiflora Bark Extract (PineXol) Decreases Adiposity in Mice by Down-Regulation of Hepatic De Novo Lipogenesis and Adipogenesis in White Adipose Tissue.

    abstract::PineXol, extracted from Korean red pine bark, has beneficial effects, such as antioxidant, antiinflammatory, and antilipogenic activities in vitro. We tested the hypothesis that PineXol supplementation could have anti-obesity effects on mice fed a high-fat diet (HFD). Four-week-old male C57BL/6 mice were fed normal ch...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1612.12037

    authors: Ahn H,Go GW

    更新日期:2017-04-28 00:00:00

  • Smilax guianensis Vitman Extract Prevents LPS-Induced Inflammation by Inhibiting the NF-κB Pathway in RAW 264.7 Cells.

    abstract::Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1911.11042

    authors: Kim JG,Kim MJ,Lee JS,Sydara K,Lee S,Byun S,Jung SK

    更新日期:2020-06-28 00:00:00

  • Yeast Surface Display of Capsid Protein VP7 of Grass Carp Reovirus: Fundamental Investigation for the Development of Vaccine Against Hemorrhagic Disease.

    abstract::VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast c...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1505.05041

    authors: Luo S,Yan L,Zhang X,Yuan L,Fang Q,Zhang YA,Dai H

    更新日期:2015-12-28 00:00:00

  • Hydoroxyhibiscone A, a novel human neutrophil elastase inhibitor from Hibiscus syriacus.

    abstract::In an ongoing investigation of compounds from natural products that exhibit anti-aging properties, hydroxyhibiscone A (1), a new furanosesquiterpenoid, together with hibiscone D (2), was isolated from the root bark of Hibiscus syriacus. Utilizing UV, IR, NMR, and MS spectroscopic analyses, these chemical structures we...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1004.04028

    authors: Ryoo IJ,Yun BS,Lee IK,Kim YH,Lee IS,Ahn JS,Bae K,Yoo ID

    更新日期:2010-08-01 00:00:00

  • Expression and biochemical characterization of the periplasmic domain of bacterial outer membrane porin TdeA.

    abstract::TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic alpha- helical barrel domain and a membrane-embedded beta-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and dr...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Kim S,Yum S,Jo WS,Lee BL,Jeong MH,Ha NC

    更新日期:2008-05-01 00:00:00

  • Anti-Obesity Effects of a Mixture of Fermented Ginseng, Bifidobacterium longum BORI, and Lactobacillus paracasei CH88 in High-Fat Diet-Fed Mice.

    abstract::Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fe...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1801.01016

    authors: Kang D,Li Z,Ji GE

    更新日期:2018-05-28 00:00:00

  • Effect of Corynebacterium glutamicum on Livestock Material Burial Treatment.

    abstract::In recent years, foot-and-mouth disease has occurred in all parts of the world. The animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. Moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1603.03056

    authors: Kim BN,Cho HS,Cha Y,Park JK,Kim G,Kim YH,Min J

    更新日期:2016-08-28 00:00:00

  • A cost analysis of microalgal biomass and biodiesel production in open raceways treating municipal wastewater and under optimum light wavelength.

    abstract::Open raceway ponds are cost-efficient for mass cultivation of microalgae compared with photobioreactors. Although low-cost options like wastewater as nutrient source is studied to overcome the commercialization threshold for biodiesel production from microalgae, a cost analysis on the use of wastewater and other incre...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1409.09019

    authors: Kang Z,Kim BH,Ramanan R,Choi JE,Yang JW,Oh HM,Kim HS

    更新日期:2015-01-01 00:00:00

  • Cloning and characterization of squalene synthase (SQS) gene from Ganoderma lucidum.

    abstract::This report provides the complete nucleotide sequences of the full-length cDNA encoding squalene synthase (SQS) and its genomic DNA sequence from a triterpene-producing fungus, Ganoderma lucidum. The cDNA of the squalene synthase (SQS) (GenBank Accession Number: DQ494674) was found to contain an open reading frame (OR...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Zhao MW,Liang WQ,Zhang DB,Wang N,Wang CG,Pan YJ

    更新日期:2007-07-01 00:00:00

  • Effect of Fc Fusion on Folding and Immunogenicity of Middle East Respiratory Syndrome Coronavirus Spike Protein.

    abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe respiratory impairment with a reported mortality rate of ~36% in humans. The absence of clinically available MERS-CoV vaccines and treatments to date has resulted in uncontrolled incidence and propagation of the virus. In vaccine design, fusion wit...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1903.03043

    authors: Chun J,Cho Y,Park KH,Choi H,Cho H,Lee HJ,Jang H,Kim KH,Oh YK,Kim YB

    更新日期:2019-05-28 00:00:00