Abstract：
:Marine sediments are a microbial biosphere with an unknown physiology, and the sediments harbor numerous distinct phylogenetic lineages of Bacteria and Archaea that are at present uncultured. In this study, the structure of the archaeal and bacterial communities was investigated in the surface and subsurface sediments of Jeju Island using a next-generation sequencing method. The microbial communities in the surface sediments were distinct from those in the subsurface sediments; the relative abundance of sequences for Thaumarchaeota, Actinobacteria, Bacteroides, Alphaproteobacteria, and Gammaproteobacteria were higher in the surface than subsurface sediments, whereas the sequences for Euryarchaeota, Acidobacteria, Firmicutes, and Deltaproteobacteria were relatively more abundant in the subsurface than surface sediments. This study presents detailed characterization of the spatial distribution of benthic microbial communities of Jeju Island and provides fundamental information on the potential interactions mediated by microorganisms with the different biogeochemical cycles in coastal sediments.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Choi H,Koh HW,Kim H,Chae JC,Park SJdoi
10.4014/jmb.1512.12036subject
Has Abstractpub_date
2016-05-28 00:00:00pages
883-90issue
5eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1512.12036journal_volume
26pub_type
杂志文章abstract：:An electron paramagnetic resonance (EPR) signal characteristic of the 5,5'-dimethyl-1-pyrroline-N-oxide (DMPO)-OH spin adduct, which is formed from the reaction of DMPO with superoxide radicals generated by xanthine oxidase-mediated reaction, was significantly reduced by the cadaverine or Escherichia coli Mn-containin...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
abstract：:Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fe...
journal_title：Journal of microbiology and biotechnology
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doi：10.4014/jmb.1801.01016
更新日期：2018-05-28 00:00:00
abstract：:In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized DEAE·HCl derivatization...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1202.02049
更新日期：2012-12-01 00:00:00
abstract：:It has been well known that the use of Saccharomyces cerevisiae can cause fungemia in critically ill patients and flavone shows an antimicrobial effect on S. cerevisiae. Therefore, we have investigated the activities of thirteen flavone analogues on S. cerevisiae in our studies. Because flavonoids including flavones h...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:An efficient and simple fermentation process was developed for the production of gamma-amminobutyric acid (GABA) by Lactobacillus sakei B2-16. When the L. sakei B2-16 was cultivated in the rice bran extracts medium containing 4% sucrose, 1% yeast extract and 12% monosodium glutamate, the maximum GABA concentration rea...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2010-04-01 00:00:00
abstract：:We isolated and functionally characterized the α- and β- subunits (ApCpnA and ApCpnB) of a chaperonin from Aeropyrum pernix K1. The constructed vectors pET3d- ApCpnA and pET21a-ApCpnB were transformed into E. coli Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) cells. The expression of ApCpnA (60.7 kDa) and ApCpnB (61.2...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1212.12041
更新日期：2013-06-28 00:00:00
abstract：:The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monocl...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1304.04011
更新日期：2013-09-28 00:00:00
abstract：:Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and termina...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:As a provirus, polydnavirus has a segmented DNA genome on chromosome(s) of host wasp. It contains several genes in each segment that presumably play critical roles in regulating physiological processes of target insect parasitized by the wasp. A cysteine-rich protein 1 (CRP1) is present in the polydnavirus Cotesia plu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.0808.495
更新日期：2009-06-01 00:00:00
abstract：:Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capabili...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1602.02042
更新日期：2016-11-28 00:00:00
abstract：:Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransu...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-06-01 00:00:00
abstract：:We inoculated different combinations of three starter candidates of Bacillus licheniformis, Staphylococcus succinus, and Tetragenococcus halophilus, into sterilized soybeans to predict their contributions to volatile compounds production through soybean fermentation. Simultaneously, we added NaCl to soybean cultures t...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1811.11012
更新日期：2019-02-28 00:00:00
abstract：:Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cell...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1107.07022
更新日期：2011-12-01 00:00:00
abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1407.07001
更新日期：2015-01-01 00:00:00
abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1507.07104
更新日期：2015-12-28 00:00:00
abstract：:The correlation between alcoholic fermentation rate, measured as carbon dioxide (CO2) evolution, and the rate of hydrogen sulfide (H2S) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace H2S were directly impacted by yeast assimilable nitrogenous ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1010.10058
更新日期：2011-05-01 00:00:00
abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1410.10023
更新日期：2015-02-01 00:00:00
abstract：:The aim of the present work was to examine the putative promoter region of the operon ansPAB and to determine the general elements required for the regulation of transcriptional activity. The transcriptional start site of the ansPAB promoter was determined by using highresolution S1-nuclease mapping. Sequence analysis...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1503.03009
更新日期：2015-08-01 00:00:00
abstract：:To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2011-04-01 00:00:00
abstract：:Interleukin-13 (IL-13) has been proposed as a therapeutic target for bronchial asthma as it plays crucial roles in the pathogenesis of the disease. We developed an in vitro test system measuring transcriptional downregulatory activities on IL-13 as a primary screening method to select drug candidates from natural prod...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2009-03-01 00:00:00
abstract：:Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other related conditions. In this study, we investigated the antimelanogenic effects of an extract from the microalgae Chlamydomonas reinhardtii (CE) and potential mechanisms responsible for its inhibi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1810.10008
更新日期：2018-12-28 00:00:00
abstract：:Staphylococcus aureus strains are important foodborne pathogens that produce various toxins. To evaluate the risk of the enterotoxins, four S. aureus strains from kinbap and two clinical samples were isolated and identified, and their expression of the enterotoxin genes were analyzed using a reverse transcription real...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:Citrobacter sp. is a cause of significant opportunistic nosocomial infection and is frequently found in human and animal feces, soil, and sewage water, and even in industrial waste or putrefaction. Biofilm formation is an important virulence trait of Citrobacter sp. pathogens but the process and characteristics of thi...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb1307.07041
更新日期：2013-12-01 00:00:00
abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1007.07015
更新日期：2010-11-01 00:00:00
abstract：:To increase the availability of microalgae as producers of valuable compounds, it is necessary to develop novel systems for gene expression regulation. Among the diverse expression systems available in microalgae, none are designed to induce expression by low temperature. In this study, we explored a cold-inducible sy...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.2007.07024
更新日期：2020-11-28 00:00:00
abstract：:The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-08-01 00:00:00
abstract：:The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALN...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1211.11044
更新日期：2013-05-01 00:00:00
abstract：:Retrovirus tropism can be restricted by host cell factors such as Fv1, TRIM5alpha, and Lv1 that inhibit infection by targeting the incoming viral capsid. The Fv1 gene inhibits murine leukemia virus infection in mice, but the precise mechanism of Fv1-mediated restriction is poorly understood. Our previous studies had d...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：
更新日期：2008-04-01 00:00:00
abstract：:White spot syndrome virus (WSSV) is a major threat to the shrimp farming industry and so far there is no effective therapy for it, and thus early diagnostic of WSSV is of great importance. However, at the early stage of infection, the extremely low-abundance of WSSV DNA challenges the detection sensitivity and accurac...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1606.06053
更新日期：2017-03-28 00:00:00
abstract：:Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CYPVdh, and CYPeryF, as templates. The lowest energy SPCYP147F1 model was then assessed for stereochemical quality and side-chain environment by Accelrys Discovery Studio 3.1 software. Further actives...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
doi：10.4014/jmb.1112.12053
更新日期：2012-07-01 00:00:00
