Construction of amylolytic industrial brewing yeast strain with high glutathione content for manufacturing beer with improved anti-staling capability and flavor.

Abstract:

:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomycopsis fibuligera ALP1 gene encoding alpha-amylase were co-expressed in industrial brewing yeast strain Y31 targeting at alpha-acetolactate synthase (AHAS) gene (ILV2) and alcohol dehydrogenase gene (ADH2), and new recombinant strain TY3 was constructed. The glutathione content from the fermentation broth of TY3 increased to 43.83 mg/l compared to 33.34 mg/l from Y31. The recombinant strain showed high alpha-amylase activity and utilized more than 46% of starch after 5 days growing on starch as sole carbon source. European Brewery Convention tube fermentation tests comparing the fermentation broth of TY3 and Y31 showed that the flavor stability index increased to 1.3 fold and residual sugar concentration were reduced by 76.8%, respectively. Due to the interruption of ILV2 gene and ADH2 gene, the amounts of off-flavor compounds diacetyl and acetaldehyde were reduced by 56.93% and 31.25%, comparing with the amounts of these from Y31 fermentation broth. In addition, as no drug-resistance genes were introduced to new recombinant strain, consequently, it should be more suitable for use in beer industry because of its better flavor stability and other beneficial characteristics.

journal_name

J Microbiol Biotechnol

authors

Wang J,Wang ZY,He XP,Zhang BR

doi

10.4014/jmb.1004.04007

subject

Has Abstract

pub_date

2010-11-01 00:00:00

pages

1539-45

issue

11

eissn

1017-7825

issn

1738-8872

pii

JMB020-11-12

journal_volume

20

pub_type

杂志文章
  • Production of platinum nanoparticles and nanoaggregates using Neurospora crassa.

    abstract::Fungal biomass and fungal extract of the nonpathogenic fungus Neurospora crassa were successfully used as reducing agents for the biosynthesis of platinum nanoparticles (PtNPs). The experiment was carried out by exposing the fungal biomass or the fungal extract to a 0.001 M precursor solution of hexachloroplatinic(IV)...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1110.10085

    authors: Castro-Longoria E,Moreno-Velázquez SD,Vilchis-Nestor AR,Arenas-Berumen E,Avalos-Borja M

    更新日期:2012-07-01 00:00:00

  • Regioselective oxidation of lauric acid by CYP119, an orphan cytochrome P450 from Sulfolobus acidocaldarius.

    abstract::Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to 85 degrees C. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We chara...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Lim YR,Eun CY,Park HG,Han S,Han JS,Cho KS,Chun YJ,Kim D

    更新日期:2010-03-01 00:00:00

  • Classification of Bacillus beneficial substances related to plants, humans and animals.

    abstract::Genus Bacillus is a spore-forming bacterium that has unique properties in cell differentiation, allowing the forming of spores in stress conditions and activated in the vegetative cell, with suitable environments occurring during the life cycle acting as a trigger. Their habitat is mainly in soil; thus, many species o...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1204.04013

    authors: Mongkolthanaruk W

    更新日期:2012-12-01 00:00:00

  • Optimization of capsular polysaccharide production by Streptococcus pneumoniae type 3.

    abstract::Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.0903.3027

    authors: Jin SD,Kim YM,Kang HK,Jung SJ,Kim D

    更新日期:2009-11-01 00:00:00

  • Latest Comprehensive Knowledge of the Crosstalk between TLR Signaling and Mycobacteria and the Antigens Driving the Process.

    abstract::Tuberculosis, which is caused by Mycobacterium tuberculosis (Mtb), is among the most pressing worldwide problems. Mtb uniquely interacts with innate immune cells through various pattern recognition receptors. These interactions initiate several inflammatory pathways that play essential roles in controlling Mtb pathoge...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1908.08057

    authors: Kim JS,Kim YR,Yang CS

    更新日期:2019-10-28 00:00:00

  • Panosialins, inhibitors of enoyl-ACP reductase from Streptomyces sp. AN1761.

    abstract::In the continued search for inhibitors of enoyl-acyl carrier protein (ACP) reductase, we found that four acylbenzenediol sulfate metabolites from Streptomyces sp. AN1761 potently inhibited bacterial enoyl-ACP reductases of Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium tuberculosis. Their structure...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1209.09038

    authors: Kwon YJ,Sohn MJ,Oh T,Cho SN,Kim CJ,Kim WG

    更新日期:2013-02-01 00:00:00

  • A technique of segment expression and RNA interference (SERI) reveals a specific physiological function of a cysteine-rich protein gene encoded in cotesia plutellae bracovirus.

    abstract::As a provirus, polydnavirus has a segmented DNA genome on chromosome(s) of host wasp. It contains several genes in each segment that presumably play critical roles in regulating physiological processes of target insect parasitized by the wasp. A cysteine-rich protein 1 (CRP1) is present in the polydnavirus Cotesia plu...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.0808.495

    authors: Barandoc K,Kim Y

    更新日期:2009-06-01 00:00:00

  • Production of alkaline protease by entrapped Bacillus licheniformis cells in repeated batch process.

    abstract::In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1105.05037

    authors: Mashhadi-Karim M,Azin M,Gargari SL

    更新日期:2011-12-01 00:00:00

  • Bacterial Outer Membrane Vesicles as a Delivery System for Virulence Regulation.

    abstract::Outer membrane vesicles (OMVs) are spherical nanostructures that are ubiquitously shed from gram-negative bacteria both in vitro and in vivo. Recent findings revealed that OMVs, which contain diverse components derived from the parent bacterium, play an important role in communication with neighboring bacteria and the...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1604.04080

    authors: Yoon H

    更新日期:2016-08-28 00:00:00

  • Comparison of traditional and commercial vinegars based on metabolite profiling and antioxidant activity.

    abstract::Metabolite profiles of seven commercial vinegars and two traditional vinegars were performed by gas chromatography time-of-flight mass spectrometry with multivariate statistical analysis. During alcohol fermentation, yeast, nuruk, and koji were used as sugars for nutrients and as fermentation substrates. Commercial an...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1408.08021

    authors: Jang YK,Lee MY,Kim HY,Lee S,Yeo SH,Baek SY,Lee CH

    更新日期:2015-02-01 00:00:00

  • Benzo[a]pyrene Alters the Expression of Genes in A549 Lung Cancer Cells and Cancer Stem Cells.

    abstract::Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a principal component of cigarette smoke. B[a]P can cause lung carcinogenesis and plays a key role in lung cancer progression. The role of B[a]P has been reported in lung cancer, but its effects on lung cancer stem cells (CSCs) have not been investigated. E...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1712.12009

    authors: Bak Y,Jang HJ,Seo JH,No SH,Chae JI,Hong J,Yoon DY

    更新日期:2018-03-28 00:00:00

  • Production of gamma-aminobutyric acid (GABA) by Lactobacillus buchneri isolated from kimchi and its neuroprotective effect on neuronal cells.

    abstract::Lactic acid bacteria that accumulated gamma-aminobutyric acid (GABA) in culture medium were screened to identify strains with high GABA-producing ability. One strain, MS, which was isolated from kimchi, showed the highest GABA-producing ability among the screened strains. MS was identified as Lactobacillus buchneri ba...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Cho YR,Chang JY,Chang HC

    更新日期:2007-01-01 00:00:00

  • Functional characterization of the α- and β-subunits of a group II chaperonin from Aeropyrum pernix K1.

    abstract::We isolated and functionally characterized the α- and β- subunits (ApCpnA and ApCpnB) of a chaperonin from Aeropyrum pernix K1. The constructed vectors pET3d- ApCpnA and pET21a-ApCpnB were transformed into E. coli Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) cells. The expression of ApCpnA (60.7 kDa) and ApCpnB (61.2...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1212.12041

    authors: Lee JW,Kim SW,Kim JH,Jeon SJ,Kwon HJ,Kim BW,Nam SW

    更新日期:2013-06-28 00:00:00

  • The Antibiosis Action and Rice-Induced Resistance, Mediated by a Lipopeptide from Bacillus amyloliquefaciens B014, in Controlling Rice Disease Caused by Xanthomonas oryzae pv. oryzae.

    abstract::In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homo...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1510.10072

    authors: Li SB,Xu SR,Zhang RN,Liu Y,Zhou RC

    更新日期:2016-04-28 00:00:00

  • Hsp20, a small heat shock protein of Deinococcus radiodurans, confers tolerance to hydrogen peroxide in Escherichia coli.

    abstract::The present study shows that DR1114 (Hsp20), a small heat shock protein of the radiationresistant bacterium Deinococcus radiodurans, enhances tolerance to hydrogen peroxide (H2O2) stress when expressed in Escherichia coli. A protein profile comparison showed that E. coli cells overexpressing D. radiodurans Hsp20 (EC-p...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1403.03006

    authors: Singh H,Appukuttan D,Lim S

    更新日期:2014-08-01 00:00:00

  • Optimal Temperature and Light Intensity for Improved Mixotrophic Metabolism of Chlorella sorokiniana Treating Livestock Wastewater.

    abstract::Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experi...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1707.07007

    authors: Lee TH,Jang JK,Kim HW

    更新日期:2017-11-28 00:00:00

  • Molecular characterization of echovirus 30-associated outbreak of aseptic meningitis in Korea in 2008.

    abstract::Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine th...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Choi YJ,Park KS,Baek KA,Jung EH,Nam HS,Kim YB,Park JS

    更新日期:2010-03-01 00:00:00

  • Sugarcane bagasse hydrolysis using yeast cellulolytic enzymes.

    abstract::Ethanol fuel production from lignocellulosic biomass is emerging as one of the most important technologies for sustainable development. To use this biomass, it is necessary to circumvent the physical and chemical barriers presented by the cohesive combination of the main biomass components, which hinders the hydrolysi...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1302.02062

    authors: Souza AC,Carvalho FP,Silva e Batista CF,Schwan RF,Dias DR

    更新日期:2013-10-28 00:00:00

  • High β-glucosidase secretion in Saccharomyces cerevisiae improves the efficiency of cellulase hydrolysis and ethanol production in simultaneous saccharification and fermentation.

    abstract::Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this st...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1305.05011

    authors: Tang H,Hou J,Shen Y,Xu L,Yang H,Fang X,Bao X

    更新日期:2013-11-28 00:00:00

  • Optimization of yeast surface-displayed cDNA library screening for low abundance targets.

    abstract::The yeast surface-displayed cDNA library has been used to identify unknown antigens. However, when unknown target antigens show moderate-to-low abundance, some modifications are needed in the screening process. In this study, a directional random-primed cDNA library was used to increase the number of candidates for th...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1409.09079

    authors: Kim JY,Kim HK,Jang HJ,Kim EK,Kim MK

    更新日期:2015-04-01 00:00:00

  • The Biosynthesis Pathway of Swainsonine, a New Anticancer Drug from Three Endophytic Fungi.

    abstract::Swainsonine (SW) is the principal toxic ingredient of locoweed plants that causes locoism characterized by a disorder of the nervous system. It has also received widespread attention in the medical field for its beneficial anticancer and antitumor activities. Endophytic fungi, Alternaria sect. Undifilum oxytropis isol...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章,评审

    doi:10.4014/jmb.1709.09003

    authors: Ren Z,Song R,Wang S,Quan H,Yang L,Sun L,Zhao B,Lu H

    更新日期:2017-11-28 00:00:00

  • An Improved Approach to Identify Bacterial Pathogens to Human in Environmental Metagenome.

    abstract::The identification of bacterial pathogens to humans is critical for environmental microbial risk assessment. However, current methods for identifying pathogens in environmental samples are limited in their ability to detect highly diverse bacterial communities and accurately differentiate pathogens from commensal bact...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.2005.05033

    authors: Yang J,Howe A,Lee J,Yoo K,Park J

    更新日期:2020-09-28 00:00:00

  • Thermal resistance and inactivation of Enterobacter sakazakii isolates during rehydration of powdered infant formula.

    abstract::Enterobacter sakazakii may be related to outbreaks of meningitis, septicemia, and necrotizing enterocolitis, mainly in neonates. To reduce the risk of E. sakazakii in baby foods, thermal characteristics for Korean E. sakazakii isolates were determined at 52, 56, and 60 degrees C in saline solution, rehydrated powdered...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Kim SH,Park JH

    更新日期:2007-02-01 00:00:00

  • Effect of pH on the formation of lysosome-alginate beads for antimicrobial activity.

    abstract::In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1410.10023

    authors: Park HJ,Min J,Ahn JM,Cho SJ,Ahn JY,Kim YH

    更新日期:2015-02-01 00:00:00

  • Unbalanced restriction impairs SOS-induced DNA repair effects.

    abstract::The contribution of a type II restriction-modification system (R-M system) to genome integrity and cell viability was investigated. We established experimental conditions which enabled the achievement of hemimethylated and unmethylated states for the specific bases of the recognition sequences of the host's DNA. To ac...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Katna A,Boratynski R,Furmanek-Blaszk B,Zolcinska N,Sektas M

    更新日期:2010-01-01 00:00:00

  • Immobilization of keratinolytic metalloprotease from Chryseobacterium sp. strain kr6 on glutaraldehyde-activated chitosan.

    abstract::Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI d...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1111.11048

    authors: Silveira ST,Gemelli S,Segalin J,Brandellli A

    更新日期:2012-06-01 00:00:00

  • Optimization of Direct Lysine Decarboxylase Biotransformation for Cadaverine Production with Whole-Cell Biocatalysts at High Lysine Concentration.

    abstract::Cadaverine (1,5-diaminopentane) is an important industrial chemical with a wide range of applications. Although there have been many efforts to produce cadaverine through fermentation, there are not many reports of the direct cadaverine production from lysine using biotransformation. Whole-cell reactions were examined...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1412.12052

    authors: Kim HJ,Kim YH,Shin JH,Bhatia SK,Sathiyanarayanan G,Seo HM,Choi KY,Yang YH,Park K

    更新日期:2015-07-01 00:00:00

  • Effective microwell plate-based screening method for microbes producing cellulase and xylanase and its application.

    abstract::Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1405.05052

    authors: Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh C

    更新日期:2014-11-28 00:00:00

  • In Vitro Antiviral Activity of Cinnamomum cassia and Its Nanoparticles Against H7N3 Influenza A Virus.

    abstract::Nanoparticles have wide-scale applications in various areas, including medicine, chemistry, electronics, and energy generation. Several physical, biological, and chemical methods have been used for synthesis of silver nanoparticles. Green synthesis of silver nanoparticles using plants provide advantages over other met...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1508.08024

    authors: Fatima M,Zaidi NU,Amraiz D,Afzal F

    更新日期:2016-01-01 00:00:00

  • Lipoteichoic Acid Isolated from Staphylococcus aureus Induces Both Epithelial-Mesenchymal Transition and Wound Healing in HaCaT Cells.

    abstract::Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There ar...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1703.03068

    authors: Kim S,Kim HE,Kang B,Lee YW,Kim H,Chung DK

    更新日期:2017-10-28 00:00:00