Abstract:
:This study compared the radioprotective effects of high-molecular-weight poly-gamma-glutamate (γ-PGA, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence intensity after binding the DNA with ethidium bromide. All the experiments used ⁶⁰Co gamma radiation at 1,252 Gy, representing 50% DNA damage. When increasing the concentration of γ-PGA from 0.33 to 1.65 μM, the DNA protection from radiation-induced damage also increased, with a maximum of 87% protection. Meanwhile, the maximal DNA protection when increasing the concentration of GSH was only 70%. Therefore, γ-PGA exhibited significant radioprotective effects against gamma irradiation.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Oh YJ,Kwak MS,Sung MHdoi
10.4014/jmb.1712.12016subject
Has Abstractpub_date
2018-04-28 00:00:00pages
527-533issue
4eissn
1017-7825issn
1738-8872pii
10.4014/jmb.1712.12016journal_volume
28pub_type
杂志文章abstract::This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1502.02057
更新日期:2015-10-01 00:00:00
abstract::In the present work, current knowledge on the potential fate, microbial degradation, and toxicity of hexahydro- 1,3,5-trinitro-1,3,5-triazine (RDX) was thoroughly reviewed, focusing on the toxicological assessment of a variety of potential RDX degradation pathways in bacteria and fungi. The present review on microbial...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1203.04002
更新日期:2012-10-01 00:00:00
abstract::Pseudomonas putida JM 37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that potentially convert glyoxylate were disrupted, namely tartronate semialdehyde synthase (gcl), malate synthase (glcB) and isocitrate lyase (aceA). Strains w...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0912.12005
更新日期:2010-06-01 00:00:00
abstract::In this study, we investigated the feasibility of producing bioethanol from the hydrolysate of rape stem. Specifically, the most ideal yeast strain was screened, and the microaeration was performed by surface aeration on a liquid medium surface. Among the yeast strains examined, Pichia stipitis CBS 7126 displayed the ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1008.08001
更新日期:2011-01-01 00:00:00
abstract::A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1405.05008
更新日期:2014-10-01 00:00:00
abstract::Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capabili...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1602.02042
更新日期:2016-11-28 00:00:00
abstract::The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A non-polar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to crea...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::With the advantages of biocatalytic method, enzymes have been excavated for the synthesis of chiral amino acids by the reductive amination of ketones, offering a promising way of producing pharmaceutical intermediates. In this work, a robust phenylalanine dehydrogenase (PheDH) with wide substrate spectrum and high cat...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1907.07015
更新日期:2020-01-28 00:00:00
abstract::The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the bind...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1506.06079
更新日期:2015-11-01 00:00:00
abstract::The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and fi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract::To obtain a natural antioxidant from a marine biomass, this study investigated the antioxidative activity of methanolic extracts from the marine brown alga, Ishige okamurae collected off Jeju Island. A potent free radical scavenging activity was detected in the ethyl acetate fraction containing polyphenolic compounds,...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterize probiotic pLR strains in human fecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rha...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1705.05055
更新日期:2017-11-28 00:00:00
abstract::Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identificat...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1503.03072
更新日期:2015-10-01 00:00:00
abstract::Trimethylamine (TMA) is a well-known off-odor compound in fish and fishery products and is a metabolic product of trimethylamine N-oxide (TMAO) generated by the enzymatic action of microorganisms. The off-odor is a factor that can debase the value of fish and fishery products. The present study aimed to remove TMA usi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2005.05007
更新日期:2020-10-28 00:00:00
abstract::The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic wa...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2009-09-01 00:00:00
abstract::Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from Paeonia lactiflora against Candida albicans, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabli...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1611.11064
更新日期:2017-02-28 00:00:00
abstract::Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17.7, cytosolic class I sHSPs, were cloned from a tropical plant, Ageratina adenophorum. Their C-terminal domains were highly conserved with those of s...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0901.0046
更新日期:2009-12-01 00:00:00
abstract::Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1611.11006
更新日期:2017-04-28 00:00:00
abstract::The use of microalgal biomass is an interesting technology for the removal of heavy metals from aqueous solutions owing to its high metal-binding capacity, but the interactions with bacteria as a strategy for the removal of toxic metals have been poorly studied. The goal of the current research was to investigate the ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1611.11059
更新日期:2017-06-28 00:00:00
abstract::Apoptosis is the process of programmed cell death executed by specific proteases, the caspases, which mediate the cleavage of various vital proteins. Elucidating the consequences of this endoproteolytic cleavage is crucial to understanding cell death and other related biological processes. Although a number of possibl...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1312.12068
更新日期:2014-05-01 00:00:00
abstract::A cDNA encoding cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum and ChainA, stefin B te...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1001.01022
更新日期:2010-08-01 00:00:00
abstract::The gut microbiome influences the health and well-being of dogs. However, little is known about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed to investigate the differences in the fecal microbiome in three breeds of dog fed and housed under the same conditions, namely eight Malte...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1906.06048
更新日期:2019-12-28 00:00:00
abstract::The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter is...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2009-07-01 00:00:00
abstract::This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory con...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::Titanium dioxide (TiO2) has wide applications in food, cosmetics, pharmaceuticals and manufacturing due to its many properties such as photocatalytic activity and stability. In this study, the biosynthesis of TiO2 nanoparticles (NPs) was achieved by using Baker's yeast. TiO2 NPs were characterized by X-ray Diffraction...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1807.07005
更新日期:2018-10-28 00:00:00
abstract::A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structur...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1708.08032
更新日期:2018-01-28 00:00:00
abstract::Lactobacillus species have been shown to enhance intestinal epithelial barrier function, modulate host immune responses, and suppress the growth of pathogenic bacteria, yeasts, molds, and viruses. Thus, lactobacilli have been used as probiotics for treating various diseases, including intestinal disorders, and as biol...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1501.01006
更新日期:2015-04-01 00:00:00