Abstract：
:Pseudomonas putida JM 37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that potentially convert glyoxylate were disrupted, namely tartronate semialdehyde synthase (gcl), malate synthase (glcB) and isocitrate lyase (aceA). Strains with transposon insertion in either of these genes were isolated from a 50,000 clone library employing a PCR-guided enrichment strategy. Further, all three respective double mutants were constructed via site-directed insertion of a knock-out plasmid. Neither mutation of gcl, glcB, aceA nor any of the respective double mutation influenced glyoxylic acid conversion, indicating that P. putida JM37 may possess other enzymes and pathways for glyoxylate metabolism.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Li XZ,Klebensberger J,Rosche Bdoi
10.4014/jmb.0912.12005subject
Has Abstractpub_date
2010-06-01 00:00:00pages
1006-10issue
6eissn
1017-7825issn
1738-8872pii
JMB020-06-09journal_volume
20pub_type
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