A genetically engineered Pseudomonas fluorescens strain possesses the dual activity against phytopathogenic fungi and insects.

abstract：:The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNAdependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular ...

journal_title：Journal of microbiology and biotechnology

authors： Kim JH,Rhee JK,Ahn DG,Kim KP,Oh JW

更新日期：2014-12-28 00:00:00

abstract：:Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total ...

journal_title：Journal of microbiology and biotechnology

authors： Siddiquee S,Tan SG,Yusof UK

更新日期：2010-09-01 00:00:00

abstract：:In this study, DEAE-corncobs [delignified corncob grits derivatized with 2-(diethylamino)ethyl chloride hydrochloride (DEAE·HCl)] were prepared as a carrier to immobilize yeast (Saccharomyces cerevisiae) for ethanol production. The immobilized yeast cell reactor produced ethanol under optimized DEAE·HCl derivatization...

journal_title：Journal of microbiology and biotechnology

authors： Lee SE,Lee CG,Kang DH,Lee HY,Jung KH

更新日期：2012-12-01 00:00:00

abstract：:Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of ...

journal_title：Journal of microbiology and biotechnology

authors： Mergulhão FJ,Monteiro GA

更新日期：2007-08-01 00:00:00

abstract：:Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identificat...

journal_title：Journal of microbiology and biotechnology

authors： Chen C,Zhong G,Ren L,Lu C,Li Z,Wu Y

更新日期：2015-10-01 00:00:00

abstract：:PineXol, extracted from Korean red pine bark, has beneficial effects, such as antioxidant, antiinflammatory, and antilipogenic activities in vitro. We tested the hypothesis that PineXol supplementation could have anti-obesity effects on mice fed a high-fat diet (HFD). Four-week-old male C57BL/6 mice were fed normal ch...

journal_title：Journal of microbiology and biotechnology

authors： Ahn H,Go GW

更新日期：2017-04-28 00:00:00

abstract：:This study examined the laxative effects of hot-water extracts of Hovenia dulcis Thunb. (HD), Phyllostachys pubescens Mazel (PM), and a 2:8 mixture of both (HP) in two chronic constipation models. For the loperamide-induced constipation model, animals were divided into an untreated group, negative control group (loper...

journal_title：Journal of microbiology and biotechnology

authors： Oh KN,Kim Y,Choi EJ,Lee H,Hong JA,Kim M,Oh DR,Jung MA,Park RD,Kim SI,Yong JS,Lee HS,Ban S,Choi CY

更新日期：2020-05-28 00:00:00

abstract：:Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There ar...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Kim HE,Kang B,Lee YW,Kim H,Chung DK

更新日期：2017-10-28 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:Bioethanol production from lignocellulose is considered as a sustainable biofuel supply. However, the low cellulose hydrolysis efficiency limits the cellulosic ethanol production. The cellulase is strongly inhibited by the major end product cellobiose, which can be relieved by the addition of β-glucosidase. In this st...

journal_title：Journal of microbiology and biotechnology

authors： Tang H,Hou J,Shen Y,Xu L,Yang H,Fang X,Bao X

更新日期：2013-11-28 00:00:00

abstract：:In this study, a total of 58 different kinds of nuruk (a traditional Korean fermentation starter) were prepared, including 46 kinds of restored nuruk from ancient documents. Each nuruk was evaluated by analysis of its saccharification power, and the enzyme activities of glucoamylase, α-amylase, β-amylase, protease, an...

journal_title：Journal of microbiology and biotechnology

authors： Lee JE,Lee AR,Kim H,Lee E,Kim TW,Shin WC,Kim JH

更新日期：2017-05-28 00:00:00

abstract：:Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7- hydroxyl GA-7-O-β-D-glucoside (1) and ACDL3172-18-O-β-D-g...

journal_title：Journal of microbiology and biotechnology

authors： Huo Q,Li HM,Lee JK,Li J,Ma T,Zhang X,Dai Y,Hong YS,Wu CZ

更新日期：2016-01-01 00:00:00

abstract：:Outer membrane vesicles (OMVs) are spherical nanostructures that are ubiquitously shed from gram-negative bacteria both in vitro and in vivo. Recent findings revealed that OMVs, which contain diverse components derived from the parent bacterium, play an important role in communication with neighboring bacteria and the...

journal_title：Journal of microbiology and biotechnology

authors： Yoon H

更新日期：2016-08-28 00:00:00

abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Xu Y,Chen X,Zhang LY

更新日期：2015-01-01 00:00:00

abstract：:Rhodococcus sp. strain p52, a previously isolated dibenzofuran degrader, could effectively inhibit the growth of cyanobacteria, including species of Microcystis, Anabaena, and Nodularia. When strain p52 was inoculated at the concentration of 7.7×10(7) CFU/ml, 93.5% of exponentially growing Microcystis aeruginosa (7.3×...

journal_title：Journal of microbiology and biotechnology

authors： Wang MH,Peng P,Liu YM,Jia RB,Li L

更新日期：2013-02-01 00:00:00

abstract：:To shed light on the genetic differences among food-originated coagulase-negative Staphylococcus (CNS), we performed pan-genome analysis of five species: Staphylococcus carnosus (two strains), Staphylococcus equorum (two strains), Staphylococcus succinus (three strains), Staphylococcus xylosus (two strains), and Staph...

journal_title：Journal of microbiology and biotechnology

authors： Heo S,Lee JS,Lee JH,Jeong DW

更新日期：2020-03-28 00:00:00

abstract：:The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing a...

journal_title：Journal of microbiology and biotechnology

authors： Long D,Tang X,Cai K,Chen G,Shen C,Shi J,Chen L,Chen Y

更新日期：2013-08-01 00:00:00

abstract：:Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which mi...

journal_title：Journal of microbiology and biotechnology

authors： Hou Q,Huang Y,Wang Y,Liao L,Zhu Z,Zhang W,Liu Y,Li P,Chen X,Liu F

更新日期：2020-10-28 00:00:00

abstract：:Trimethylamine (TMA) is a well-known off-odor compound in fish and fishery products and is a metabolic product of trimethylamine N-oxide (TMAO) generated by the enzymatic action of microorganisms. The off-odor is a factor that can debase the value of fish and fishery products. The present study aimed to remove TMA usi...

journal_title：Journal of microbiology and biotechnology

authors： Park SK,Jo DM,Yu D,Khan F,Lee YB,Kim YM

更新日期：2020-10-28 00:00:00

abstract：:Staphylococcus aureus strains are important foodborne pathogens that produce various toxins. To evaluate the risk of the enterotoxins, four S. aureus strains from kinbap and two clinical samples were isolated and identified, and their expression of the enterotoxin genes were analyzed using a reverse transcription real...

journal_title：Journal of microbiology and biotechnology

authors： Lee YD,Moon BY,Park JH,Chang HI,Kim WJ

更新日期：2007-03-01 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:The Streptomyces coelicolor M145 genome harbors six copies of divergent rRNA operons that differ at ~0.2% and ~0.6% of the nucleotide positions in small subunit (SSU) and large subunit (LSU) rRNA genes, respectively. When these rRNA genes are expressed, a single cell may harbor three different kinds of SSU rRNA and fi...

journal_title：Journal of microbiology and biotechnology

authors： Kim HL,Shin E,Kim HM,Go H,Roh J,Bae J,Lee K

更新日期：2007-10-01 00:00:00

abstract：:The gene encoding α-L-arabinofuranosidase (AFase) from Paenibacillus sp. DG-22 was cloned, sequenced, and expressed in Escherichia coli. The AFase gene (abfA) comprises a 1,509 bp open reading frame encoding 502 amino acids with a molecular mass of 56,520 daltons. The deduced amino acid sequence of the gene shows that...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Lee YE

更新日期：2014-02-28 00:00:00

abstract：:Total 121 lactic acid bacteria were isolated from homemade Inner Mongolia extra hard Hurood cheese. Seven of these strains, identified as Lactobacillus plantarum, were studied for probiotic characteristics. All seven strains survived at pH 3.0 for 3 h, or in the presence of oxgall at 0.3% or 0.6% for 4 h, but their vi...

journal_title：Journal of microbiology and biotechnology

authors： Zhang J,Zhang X,Zhang L,Zhao Y,Niu C,Yang Z,Li S

更新日期：2014-02-28 00:00:00

abstract：:Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural enviro...

journal_title：Journal of microbiology and biotechnology

authors： Shi S,Yang L,Yang C,Li S,Zhao H,Ren L,Wang X,Lu F,Li Y,Zhao H

更新日期：2020-12-11 00:00:00

abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...

journal_title：Journal of microbiology and biotechnology

authors： Zheng CJ,Sohn MJ,Chi SW,Kim WG

更新日期：2010-05-01 00:00:00

abstract：:Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The ...

journal_title：Journal of microbiology and biotechnology

authors： Hong I,Kim YS,Choi SG

更新日期：2010-02-01 00:00:00

abstract：:The objectives of this study were to determine the antibiotypes, SCCmec subtypes, PVL carriage, and genetic diversity of MRSA strains from a tertiary hospital. Sixtysix MRSA strains were selected randomly (2003, 2004, and 2007) and tested for the Panton-Valentine leukocidin gene, mecA gene, and SCCmec type via a PCR. ...

journal_title：Journal of microbiology and biotechnology

authors： Thong KL,Junnie J,Liew FY,Yusof MY,Hanifah YA

更新日期：2009-10-01 00:00:00

abstract：:A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Ten IL,Jung HM,Liu QM,Im WT,Lee ST

更新日期：2008-06-01 00:00:00

abstract：:The effect of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), on the horseradish peroxidase (HRP)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide (H2O2) was investigated. HRP maintains its high activity in the aqueous mixtures containing various conc...

journal_title：Journal of microbiology and biotechnology

authors： Lee YM,Kwon OY,Yoo IK,Ryu KG

更新日期：2007-04-01 00:00:00