Abstract：
:Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The 147-bp fragment of the MDCD-1L gene encoding an additional methionine residue was subcloned into the pTYB11 vector. Recombinant MDCD-1L was expressed as an intein fusion protein in E. coli, and then purified by affinity chromatography using chitin beads. A small peptide with a molecular mass of about 5 kDa was detected by tricine gel electrophoresis. The recombinant MDCD-1L peptide was purified from the gel and its amino acid sequence was determined by nanoLC-ESI-MS/MS analysis. The initiating amino acid, methionine, remained attached to the N-terminal region of recombinant MDCD-1L. Purified MDCD-1L showed antimicrobial activity against a Micrococcus luteus test strain.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Hong I,Kim YS,Choi SGsubject
Has Abstractpub_date
2010-02-01 00:00:00pages
350-5issue
2eissn
1017-7825issn
1738-8872pii
JMB020-02-17journal_volume
20pub_type
杂志文章abstract：:Phenol-soluble modulins (PSMs) are responsible for regulating biofilm formation, persister cell formation, pmtR expression, host cell lysis, and anti-bacterial effects. To determine the effect of psm deletion on methicillin-resistant Staphylococcus aureus, we investigated psm deletion mutants including Δpsmα, Δpsmβ, a...
journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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pub_type： 杂志文章
doi：10.4014/jmb.1107.07063
更新日期：2012-05-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2020-01-28 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2014-06-28 00:00:00
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journal_title：Journal of microbiology and biotechnology
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doi：
更新日期：2007-02-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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