Anaerobic lipid degradation through acidification and methanization.

abstract：:To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using ...

journal_title：Journal of microbiology and biotechnology

authors： Lim MY,Lee HJ,Kim P

更新日期：2011-04-01 00:00:00

abstract：:In this study, three of the representative EDCs, 17beta-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein d...

journal_title：Journal of microbiology and biotechnology

authors： Kim YS,Min J,Hongz HN,Park JH,Park KS,Gu MB

更新日期：2007-08-01 00:00:00

abstract：:The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

journal_title：Journal of microbiology and biotechnology

authors： Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

更新日期：2013-08-01 00:00:00

abstract：:In this study, we investigated the potential of using sediment bioelectrochemical systems (SBESs) for in situ treatment of the water and sediment in brackish aquaculture ponds polluted with uneaten feed. An SBES integrated into a laboratory-scale tank simulating a brackish aquaculture pond was established. This test t...

journal_title：Journal of microbiology and biotechnology

authors： Pham HT,Tran HT,Vu LT,Dang HT,Nguyen TTT,Dang THT,Nguyen MTT,Nguyen HQ,Kim BH

更新日期：2019-07-28 00:00:00

abstract：:This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermen...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Xu Y,Chen X,Zhang LY

更新日期：2015-01-01 00:00:00

abstract：:Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter is...

journal_title：Journal of microbiology and biotechnology

authors： Shin E,Lee Y

更新日期：2009-07-01 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:The purified endochitosanase (Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers (GlcN)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this e...

journal_title：Journal of microbiology and biotechnology

authors： Gao XA,Jung WJ,Kuk JH,Park RD

更新日期：2009-04-01 00:00:00

abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...

journal_title：Journal of microbiology and biotechnology

authors： Lim HD,Cheong DE,Shin HJ,Kim GJ

更新日期：2010-11-01 00:00:00

abstract：:TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic alpha- helical barrel domain and a membrane-embedded beta-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and dr...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Yum S,Jo WS,Lee BL,Jeong MH,Ha NC

更新日期：2008-05-01 00:00:00

abstract：:In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which th...

journal_title：Journal of microbiology and biotechnology

authors： Mashhadi-Karim M,Azin M,Gargari SL

更新日期：2011-12-01 00:00:00

abstract：:The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-5-methyl-Omethyltyrosine (3h5mOmTyr), a nonproteinogenic amino acid, is the precursor of the tetrahydroisoquinoline molecular core. In the biosynthetic gene cluste...

journal_title：Journal of microbiology and biotechnology

authors： Fu CY,Tang MC,Peng C,Li L,He YL,Liu W,Tang GL

更新日期：2009-05-01 00:00:00

abstract：:Outer membrane vesicles (OMVs) are spherical nanostructures that are ubiquitously shed from gram-negative bacteria both in vitro and in vivo. Recent findings revealed that OMVs, which contain diverse components derived from the parent bacterium, play an important role in communication with neighboring bacteria and the...

journal_title：Journal of microbiology and biotechnology

authors： Yoon H

更新日期：2016-08-28 00:00:00

abstract：:Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...

journal_title：Journal of microbiology and biotechnology

authors： Lee JM,Hwang KT,Jun WJ,Park CS,Lee MY

更新日期：2008-10-01 00:00:00

abstract：:An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...

journal_title：Journal of microbiology and biotechnology

authors： Joo HS,Choi JW

更新日期：2012-01-01 00:00:00

abstract：:Fibrinolytic enzyme genes (aprE2, aprE176, and aprE179) were introduced into the Bacillus subtilis 168 chromosome without any antibiotic resistance gene. An integration vector, pDG1662, was used to deliver the genes into the amyE site of B. subtilis 168. Integrants, SJ3-5nc, SJ176nc, and SJ179nc, were obtained after t...

journal_title：Journal of microbiology and biotechnology

authors： Jeong SJ,Park JY,Lee JY,Lee KW,Cho KM,Kim GM,Shin JH,Kim JS,Kim JH

更新日期：2015-11-01 00:00:00

abstract：:Colorectal cancer (CRC) is regarded as one of the most common and deadly forms of cancer. Gut microbiota is vital to retain and promote several functions of intestinal. Although previous researches have shown that some gut microbiota have the abilities to inhibit tumorigenesis and prevent cancer from progressing, they...

journal_title：Journal of microbiology and biotechnology

authors： Chen C,Li H

更新日期：2020-11-28 00:00:00

abstract：:Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU...

journal_title：Journal of microbiology and biotechnology

authors： Cho KM,Kwon EJ,Kim SK,Kambiranda DM,Math RK,Lee YH,Kim J,Yun HD,Kim H

更新日期：2009-08-01 00:00:00

abstract：:The contribution of a type II restriction-modification system (R-M system) to genome integrity and cell viability was investigated. We established experimental conditions which enabled the achievement of hemimethylated and unmethylated states for the specific bases of the recognition sequences of the host's DNA. To ac...

journal_title：Journal of microbiology and biotechnology

authors： Katna A,Boratynski R,Furmanek-Blaszk B,Zolcinska N,Sektas M

更新日期：2010-01-01 00:00:00

abstract：:Ralstonia solanacearum causes bacterial wilt in a wide variety of host plant species and produces a melanin-like blackish-brown pigment in stationary phase when grown in minimal medium supplemented with tyrosine. To study melanin production regulation in R. solanacearum, five mutants exhibiting overproduction of melan...

journal_title：Journal of microbiology and biotechnology

authors： Ahmad S,Lee SY,Khan R,Kong HG,Son GJ,Roy N,Choi K,Lee SW

更新日期：2017-09-28 00:00:00

abstract：:The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monocl...

journal_title：Journal of microbiology and biotechnology

authors： Rodrigues ME,Costa AR,Fernandes P,Henriques M,Cunnah P,Melton DW,Azeredo J,Oliveira R

更新日期：2013-09-28 00:00:00

abstract：:Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. c...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Kim Y,Yoon SH

更新日期：2019-03-28 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive diseas...

journal_title：Journal of microbiology and biotechnology

authors： Jung SJ,Seo ES,Yun SI,Minh BN,Jin SD,Ryu HJ,Kim D

更新日期：2011-07-01 00:00:00

abstract：:The impact of overproduction of a heterologous protein on the metabolic system of host Lactococcus lactis was investigated. The protein expression profiles of L. lactis IL1403 containing two near-identical plasmids that expressed high- and low-level of the green fluorescent protein (GFP) were examined via shotgun prot...

journal_title：Journal of microbiology and biotechnology

authors： Kim M,Jin Y,An HJ,Kim J

更新日期：2017-07-28 00:00:00

abstract：:This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Yin H,Xu X,Cheng Y,Cai Y,Wang R

更新日期：2015-10-01 00:00:00

abstract：:As an opportunistic bacterial pathogen, Pseudomonas aeruginosa PAO1 contains two phenazineproducing gene operons, phzA1B1C1D1E1F1G1 (phz1) and phzA2B2C2D2E2F2G2 (phz2), each of which is independently capable of encoding all enzymes for biosynthesizing phenazines, including phenazine-1-carboxylic acid and its derivativ...

journal_title：Journal of microbiology and biotechnology

authors： He Q,Feng Z,Wang Y,Wang K,Zhang K,Kai L,Hao X,Yu Z,Chen L,Ge Y

更新日期：2019-08-28 00:00:00

abstract：:Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a principal component of cigarette smoke. B[a]P can cause lung carcinogenesis and plays a key role in lung cancer progression. The role of B[a]P has been reported in lung cancer, but its effects on lung cancer stem cells (CSCs) have not been investigated. E...

journal_title：Journal of microbiology and biotechnology

authors： Bak Y,Jang HJ,Seo JH,No SH,Chae JI,Hong J,Yoon DY

更新日期：2018-03-28 00:00:00

abstract：:Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total ...

journal_title：Journal of microbiology and biotechnology

authors： Siddiquee S,Tan SG,Yusof UK

更新日期：2010-09-01 00:00:00

abstract：:This study aimed to characterize CTX-M producers of urinary E. coli and K. pneumoniae isolates and to determine the prevalence of plasmid-mediated antimicrobial resistance genes among them. Minimum inhibitory concentrations (MICs) were determined, and PCR and sequencing were performed. Among the 42 (82.3%) E. coli and...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Sung JY,Cho HH,Kwon KC,Koo SH

更新日期：2014-06-28 00:00:00

abstract：:The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71+/-4...

journal_title：Journal of microbiology and biotechnology

authors： Kim KM,Kim MJ,Kim DH,Park YS,Kang JS

更新日期：2009-09-01 00:00:00