Abstract:
:To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using double-crossover, for use as a screening host. The W3110 dsdA::tet strain was not able to grow in a medium containing D-serine as a sole carbon source, whereas wild-type W3110 and the complement W3110 dsdA::tet strain containing a dsdA-expression plasmid were able to grow. After introducing metagenome libraries into the screening host, a strain containing a 40-kb DNA fragment obtained from the metagenomic souce derived from a compost was selected based on its capability to grow on the agar plate containing D-serine as a sole carbon source. For identification of the genetic resource responsible for the D-serine degrading capability, transposon- micron was randomly inserted into the 40-kb metagenome. Two strains that had lost their D-serine degrading ability were negatively selected, and the two 6-kb contigs responsible for the D-serine degrading capability were sequenced and deposited (GenBank code: HQ829474.1 and HQ829475.1). Therefore, new alternative genetic resources for D-serine dehydratase was found from the metagenomic resource, and the corresponding ORFs are discussed.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Lim MY,Lee HJ,Kim Psubject
Has Abstractpub_date
2011-04-01 00:00:00pages
374-8issue
4eissn
1017-7825issn
1738-8872pii
JMB021-04-06journal_volume
21pub_type
杂志文章abstract::Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1611.11006
更新日期:2017-04-28 00:00:00
abstract::The bioassay-guided fractionation of MeOH extract from Naematoloma fasciculare afforded a petroleum ether fraction (NFPF) and four known compounds, which showed good antitumor activities to inhibit MCF-7 cell line proliferation in vitro and tumor growth in H(22) implanted mice in vivo. In addition, a number of unsatur...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2009-10-01 00:00:00
abstract::Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1004.04007
更新日期:2010-11-01 00:00:00
abstract::The infection of pandemic influenza viruses such as swine flu (H1N1) and avian flu viruses to the host cells is related to the following two factors: First, the surface protein such as HA (hemagglutinin) and NA (neuraminidase) of the influenza virus. Second, the specific structure of the oligosaccharide [sialic acid(a...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1009.09013
更新日期:2011-05-01 00:00:00
abstract::To shed light on the genetic differences among food-originated coagulase-negative Staphylococcus (CNS), we performed pan-genome analysis of five species: Staphylococcus carnosus (two strains), Staphylococcus equorum (two strains), Staphylococcus succinus (three strains), Staphylococcus xylosus (two strains), and Staph...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1910.10049
更新日期:2020-03-28 00:00:00
abstract::Two alternative pathways for methionine biosynthesis are known in Corynebacterium glutamicum: one involving transsulfuration (mediated by metB and metC) and the other involving direct sulthydrylation (mediated by metY). In this study, MetB (cystathionine gamma-synthase) and MetY (O-acetylhomoserine sulfhydrylase) from...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2008.08048
更新日期:2020-12-31 00:00:00
abstract::As a provirus, polydnavirus has a segmented DNA genome on chromosome(s) of host wasp. It contains several genes in each segment that presumably play critical roles in regulating physiological processes of target insect parasitized by the wasp. A cysteine-rich protein 1 (CRP1) is present in the polydnavirus Cotesia plu...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0808.495
更新日期:2009-06-01 00:00:00
abstract::The evolution of living organisms occurs via a combination of highly complicated processes that involve modification of various features such as appearance, metabolism and sensing systems. To understand the evolution of life, it is necessary to understand how each biological feature has been optimized in response to n...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0905.05017
更新日期:2009-11-01 00:00:00
abstract::Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 days. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1704.04029
更新日期:2017-08-28 00:00:00
abstract::A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract::Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0802.130
更新日期:2009-02-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic human pathogen that produces and secretes exopolysaccharides (EPS), in which cells are embedded to form a highly organized community structure called biofilm. Here, we characterized the role of cyclic diguanylate (c-di-GMP) and EPS (PEL) overproduction in the wspF mutant phen...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1405.05052
更新日期:2014-11-28 00:00:00
abstract::An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protei...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0911.11022
更新日期:2010-04-01 00:00:00
abstract::An investigation was conducted on the enhancement of production and purification of an oxidant and SDS-stable alkaline protease (BHAP) secreted by an alkalophilic Bacillus horikoshii, which was screened from the body fluid of a unique Korean polychaeta (Periserrula leucophryna) living in the tidal mud flats of Kwangwh...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1109.09006
更新日期:2012-01-01 00:00:00
abstract::Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. ga...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1604.04011
更新日期:2016-09-28 00:00:00
abstract::Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to 85 degrees C. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We chara...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-03-01 00:00:00
abstract::Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The synergy effect of trisodium phosphate (TSP) and ethanol against murine norovirus 1 (MNV-1), as a surrogate for human noroviruses, on fresh produces was evaluated. More than 2% (w/v) of TSP effectively inactivated MNV-1. The single treatment of 1% TSP or 30% ethanol for 30 min was not effective on MNV-1; however, c...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1503.03032
更新日期:2015-12-28 00:00:00
abstract::It has been well known that the use of Saccharomyces cerevisiae can cause fungemia in critically ill patients and flavone shows an antimicrobial effect on S. cerevisiae. Therefore, we have investigated the activities of thirteen flavone analogues on S. cerevisiae in our studies. Because flavonoids including flavones h...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::The total methylotrophic population associated with rice plants from different cultivars was enumerated at three different stages: vegetative, flowering, and harvesting. The bacterial population in the leaf, rhizosphere soil, endophytic in the stem and roots, and epiphytic in the florets and grains were determined fro...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract::Fermentation with filamentous fungi in a bioreactor is a complex dynamic process that is affected by flow conditions and the evolution of the rheological properties of the medium. These properties are mainly affected by the biomass concentration and the morphology of the fungus. In this work, the rheological propertie...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2012-11-01 00:00:00
abstract::Glucose dehydrogenase (GDH) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. In this study, the glucose 1-dehydrogenase B gene (gdhB) was cloned from Bacillus thuringiensis subsp. kurstaki, and wild-type (GDH-BTWT) and His-tagged (GDH-BTN-...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1603.03021
更新日期:2016-10-28 00:00:00
abstract::Bacterial resistance to antibiotics has been emerged as a major health problem in the recent years. Silver nanoparticles (AgNPs) have been known to have an inhibitory effect to the bacterial growth, however, the strength of this antibacterial activity in correlation with the physical properties of these AgNPs has bare...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1806.05089
更新日期:2018-07-19 00:00:00
abstract::A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-06-01 00:00:00
abstract::We previously reported that the CopA3 peptide (LLCIALRKK, D-form) originally isolated from the Korean dung beetle has antimicrobial and immunosuppressive effects. However, the high cost of producing the synthetic peptide, especially the D-form, has limited the development of CopA3 for therapeutic purposes. Here, we in...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1607.07065
更新日期:2017-02-28 00:00:00
abstract::Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::A novel psychrotolerant Psychrobacillus strain PB01, isolated from an Antarctic iceberg, was comparatively analyzed with five related strains. The complete genome of strain PB01 consists of a single circular chromosome (4.3 Mb) and a plasmid (19 Kb). As potential low-temperature adaptation strategies, strain PB01 has ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1909.09008
更新日期:2020-02-28 00:00:00
abstract::Marine sediments are a microbial biosphere with an unknown physiology, and the sediments harbor numerous distinct phylogenetic lineages of Bacteria and Archaea that are at present uncultured. In this study, the structure of the archaeal and bacterial communities was investigated in the surface and subsurface sediments...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1512.12036
更新日期:2016-05-28 00:00:00