Abstract:
:A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA gene sequencing. The bacterial strain, Bacillus sp. RRM1, produced a high level of protease using easily available, inexpensive agricultural residues solid-state fermentation (SSF). Among them, wheat bran was found to be the best substrate. Influences of process parameters such as moistening agents, moisture level, temperature, inoculum concentration, and co-carbon and co-nitrogen sources on the fermentation were also evaluated. Under optimized conditions, maximum protease production (i.e., 2081 U/g) was obtained from wheat bran, which is about 2-fold greater than the initial conditions. The protease enzyme was stable over a temperature range of 30-60 degrees C and pH 6-12, with maximum activity at 50 degrees C and pH 9.0. Whereas the metal ions Na+, Ca2+, and K+ enhanced the activity of the enzyme, others such as Hg2+, Cu2+, Fe2+, Co2+, and Zn2+ had rendered negative effects. The activity of the enzyme was inhibited by EDTA and enhanced by Cu2+ ions, thus indicating the nature of the enzyme as a metalloprotease. The enzyme showed extreme stability and activity even in the presence of detergents, surfactants, and organic solvents. Moreover, the present findings opened new vistas in the utilization of wheat bran, a cheap, abundantly available, and effective waste as a substrate for SSF.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Rajkumar R,Kothilmozhian J,Ramasamy Rsubject
Has Abstractpub_date
2011-06-01 00:00:00pages
627-36issue
6eissn
1017-7825issn
1738-8872pii
JMB021-06-11journal_volume
21pub_type
杂志文章abstract::Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-10-01 00:00:00
abstract::Citrobacter sp. is a cause of significant opportunistic nosocomial infection and is frequently found in human and animal feces, soil, and sewage water, and even in industrial waste or putrefaction. Biofilm formation is an important virulence trait of Citrobacter sp. pathogens but the process and characteristics of thi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb1307.07041
更新日期:2013-12-01 00:00:00
abstract::The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups o...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1007.07060
更新日期:2011-02-01 00:00:00
abstract::An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structur...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1708.08032
更新日期:2018-01-28 00:00:00
abstract::Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticu...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1607.07068
更新日期:2017-03-28 00:00:00
abstract::Interleukin-13 (IL-13) has been proposed as a therapeutic target for bronchial asthma as it plays crucial roles in the pathogenesis of the disease. We developed an in vitro test system measuring transcriptional downregulatory activities on IL-13 as a primary screening method to select drug candidates from natural prod...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract::Platycodon grandiflorum root (Platycodi radix) saponins, platycosides, have been used as health supplements and food items for the treatment of respiratory disorders and pulmonary diseases. Deglycosylated saponins have been known to exert stronger biological effects than their glycosylated forms. In the present study,...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2001.01041
更新日期:2020-06-28 00:00:00
abstract::A Gram-positive and endospore-forming strain, JH8T, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176T (96.9%...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-02-01 00:00:00
abstract::The effects of two different sugars (glucose and xylose) on the expression levels and patterns of xylose reductase (xyl1), xylitol dehydrogenase (xyl2) and xylulokinase (xyl3) genes were analyzed using Pichia stipitis. A significant increase in mRNA levels of xyl1 was observed after 6 hours growth in culture condition...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0912.12028
更新日期:2010-05-01 00:00:00
abstract::Nutraceutical treatments can reduce inflammation and prevent the development of inflammatory diseases. In this study, the anti-inflammatory effects of Smilax guianensis Vitman extract (SGE) were examined. SGE suppressed lipopolysaccharide (LPS)-mediated nitrite production in RAW 264.7 cells. SGE also prevented the LPS...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1911.11042
更新日期:2020-06-28 00:00:00
abstract::Silkworm hemolymph (SH), prepared from fifth-instar larvae of Bombyx mori and heat-treated at 60 degrees C for 30 min, was used to improve cell viability and the production of human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) in transgenic Oryza sativa L. cell suspension cultures. Even though SH could n...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2008.08048
更新日期:2020-12-31 00:00:00
abstract::Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Abelmoschus manihot (Linn.) is a medicinal herbal plant that is commonly used to treat chronic kidney disease and hepatitis. However, its effect on cell proliferation has not been clearly revealed. In this report, we sought to determine the effect of the flower extract of A. manihot (FA) on cell proliferation. Based o...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2002.02024
更新日期:2020-07-28 00:00:00
abstract::A bacterial strain, labeled UR11T, was isolated from green alga Ulva pertusa collected from Jeju Island, Korea. UR11T was identified as a gram-negative, rod-shaped, motile by gliding and aerobic bacterial strain with yellow colonies on R2A plates. The strain UR11T grew over at a temperature range of 10°C to 30°C (opti...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1812.12011
更新日期:2019-08-28 00:00:00
abstract::Trimethylamine (TMA) is a well-known off-odor compound in fish and fishery products and is a metabolic product of trimethylamine N-oxide (TMAO) generated by the enzymatic action of microorganisms. The off-odor is a factor that can debase the value of fish and fishery products. The present study aimed to remove TMA usi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2005.05007
更新日期:2020-10-28 00:00:00
abstract::Tuberculosis, which is caused by Mycobacterium tuberculosis (Mtb), is among the most pressing worldwide problems. Mtb uniquely interacts with innate immune cells through various pattern recognition receptors. These interactions initiate several inflammatory pathways that play essential roles in controlling Mtb pathoge...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章,评审
doi:10.4014/jmb.1908.08057
更新日期:2019-10-28 00:00:00
abstract::A novel cry2Ab gene was cloned and sequenced from the indigenous isolate of Bacillus thuringiensis subsp. kurstaki. This gene was designated as cry2Ab25 and its sequence revealed an open reading frame of 1,902 bp encoding a 633 aa protein with calculated molecular mass of 70 kDa and pI value of 8.98. The amino acid se...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1108.08061
更新日期:2012-01-01 00:00:00
abstract::With the advantages of biocatalytic method, enzymes have been excavated for the synthesis of chiral amino acids by the reductive amination of ketones, offering a promising way of producing pharmaceutical intermediates. In this work, a robust phenylalanine dehydrogenase (PheDH) with wide substrate spectrum and high cat...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1907.07015
更新日期:2020-01-28 00:00:00
abstract::Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::The task of improving a fungal strain is highly time-consuming due to the requirement of a large number of flasks in order to obtain a library with enough diversity. In addition, fermentations (particularly those for fungal cells) are typically performed in high-volume (100-250 ml) shake-flasks. In this study, for lar...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1610.10037
更新日期:2017-01-28 00:00:00
abstract::Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which mi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2002.02059
更新日期:2020-10-28 00:00:00
abstract::It has been well known that the use of Saccharomyces cerevisiae can cause fungemia in critically ill patients and flavone shows an antimicrobial effect on S. cerevisiae. Therefore, we have investigated the activities of thirteen flavone analogues on S. cerevisiae in our studies. Because flavonoids including flavones h...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1301.01018
更新日期:2013-04-01 00:00:00
abstract::Mycophenolic acid (MPA) is an antibiotic produced by Penicillium brevicompactum. MPA has antifungal, antineoplastic, and immunosuppressive functions, among others. β-Hydroxy-β-methylglutaryl-CoA (HMG-CoA) lyase is a key enzyme in the bypass metabolic pathway. The inhibitory activity of HMG-CoA lyase increases the MPA ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1603.03038
更新日期:2016-11-28 00:00:00
abstract::With emphasis on thermal behavior in presence of different pH conditions and salts, the kinetic and thermodynamic parameters of purified polygalacturonase (PG) of E. carotovora subsp. carotovora (Ecc) BR1 were studied since characterization of an enzyme is significant in the context of burgeoning biotechnological appl...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0908.08008
更新日期:2010-07-01 00:00:00
abstract::We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1007.07015
更新日期:2010-11-01 00:00:00
abstract::Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1907.07045
更新日期:2019-01-28 00:00:00
abstract::The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protectio...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2002.02025
更新日期:2020-07-28 00:00:00
abstract::Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC1129...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1202.02027
更新日期:2012-08-01 00:00:00