Abstract：
:The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups of either the branched or linear type of dextran with GMA. Dex-GMA microspheres loaded with hIFN-alpha was then prepared by the water-in-water emulsion technique. In vitro release and Western blotting experiments demonstrated the retained activity of hIFN-alpha released from branched dextran microspheres at 24 h by inducing phosphorylation of signal transducer and activator transcription-1 (STAT-1), a down-stream effector of IFN-alpha, in HepG2 cells. Animal data further revealed a peak of plasma levels of IFN-alpha in rats injected intravenously with BDM-hIFN-alpha at 10 min post-injection, but a sharp decline at 2 h. High plasma levels of neopterin, a plasma protein induced by IFN-alpha, were also detected in rats injected with BDM-hIFN-alpha at 10 min post-injection. Notably, plasma levels of neopterin remained high at 4 h, but largely declined thereafter.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Hong H,Jo JR,Yeon JH,Hong JT,Jung KH,Yoo SK,Jang BCdoi
10.4014/jmb.1007.07060subject
Has Abstractpub_date
2011-02-01 00:00:00pages
176-82issue
2eissn
1017-7825issn
1738-8872pii
JMB021-02-09journal_volume
21pub_type
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