Optimized substrate concentrations for production of long-chain isomaltooligosaccharides using dextransucrase of Leuconostoc mesenteroides B-512F.

Abstract:

:Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransucrase of Leuconostoc mesenteroides B-512F by changing the ratio of sucrose to maltose (varying as 1:4, 1:2, 1:1, and 2:1) and amount of each sugar (from 2% to 20%). As a result, a ratio of 2:1 (sucrose to maltose, 10:5% or 20:10%, w/v) was determined as an optimal condition for long-chain IMO synthesis (DP3-DP9) with relatively higher yields (70-90%, respectively).

journal_name

J Microbiol Biotechnol

authors

Lee MS,Cho SK,Eom HJ,Kim SY,Kim TJ,Han NS

subject

Has Abstract

pub_date

2008-06-01 00:00:00

pages

1141-5

issue

6

eissn

1017-7825

issn

1738-8872

pii

7442

journal_volume

18

pub_type

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