Bioethanol production from the hydrolysate of rape stem in a surface-aerated fermentor.

abstract：:An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structur...

journal_title：Journal of microbiology and biotechnology

authors： Wu Y,Ma Y,Li L,Yang X

更新日期：2018-01-28 00:00:00

abstract：:Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. c...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Kim Y,Yoon SH

更新日期：2019-03-28 00:00:00

abstract：:Trichoderma reesei is the major filamentous fungus used to produce cellulase and there is huge interest in promoting its ability to produce higher titers of cellulase. Among the many factors affecting cellulase production in T. reesei, the mycelial phenotype is important but seldom studied. Herein, a close homolog of ...

journal_title：Journal of microbiology and biotechnology

authors： Gao F,Li M,Liu W,Bai Y,Tu T,Wang Y,Zhang J,Luo H,Yao B,Huang H,Su X

更新日期：2020-02-28 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive diseas...

journal_title：Journal of microbiology and biotechnology

authors： Jung SJ,Seo ES,Yun SI,Minh BN,Jin SD,Ryu HJ,Kim D

更新日期：2011-07-01 00:00:00

abstract：:Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...

journal_title：Journal of microbiology and biotechnology

authors： Park IH,Kim SH,Lee YS,Lee SC,Zhou Y,Kim CM,Ahn SC,Choi YL

更新日期：2009-02-01 00:00:00

abstract：:The gut microbiome influences the health and well-being of dogs. However, little is known about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed to investigate the differences in the fecal microbiome in three breeds of dog fed and housed under the same conditions, namely eight Malte...

journal_title：Journal of microbiology and biotechnology

authors： Reddy KE,Kim HR,Jeong JY,So KM,Lee S,Ji SY,Kim M,Lee HJ,Lee S,Kim KH,Kim M

更新日期：2019-12-28 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:Based on its α-amylase activity at pH 5.0 and optimal pH of the crude enzyme, a strain (named B-5) with acid α-amylase production was screened. The B-5 strain was identified as Bacillus amyloliquefaciens through morphological, physiological, and biochemical characteristics analysis, as well as 16S rDNA phylogenetic an...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Jia W,An Y,Cheng K,Wang M,Yang S,Chen H

更新日期：2015-06-01 00:00:00

abstract：:Acetic acid is indirectly involved in cell center metabolism, and acetic acid metabolism is the core of central metabolism, affecting and regulating the production of bacteriocin. Bacteriocin is a natural food preservative that has been used in the meat and dairy industries and winemaking. In this paper, the effects o...

journal_title：Journal of microbiology and biotechnology

authors： Ge J,Kang J,Ping W

更新日期：2019-09-28 00:00:00

abstract：:Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU...

journal_title：Journal of microbiology and biotechnology

authors： Cho KM,Kwon EJ,Kim SK,Kambiranda DM,Math RK,Lee YH,Kim J,Yun HD,Kim H

更新日期：2009-08-01 00:00:00

abstract：:Organic solvent-tolerant (OST) enzymes are widely applied in various industries due to their activity and stability in organic solvents, higher substrate solubility, and increased stereo-selectivity. However, the criteria for identifying OST enzymes largely remain unresolved. In this study, we compared the amino acid ...

journal_title：Journal of microbiology and biotechnology

authors： Dachuri VK,Lee C,Jang SH

更新日期：2018-09-28 00:00:00

abstract：:It has been reported that Tat-SOD can be directly transduced into mammalian cells and skin and acts as a potential therapeutic protein in various diseases. To isolate the compound that can enhance the transduction efficiency of Tat-SOD, we screened a number of natural products. 3-O-[beta-D-Glucopyranosyl(1-->4)-alpha-...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Kim SY,Kim DW,Jang SH,Lim SS,Kwon HJ,Kang TC,Won MH,Kang IJ,Lee KS,Park J,Eum WS,Choi SY

更新日期：2008-09-01 00:00:00

abstract：:Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...

journal_title：Journal of microbiology and biotechnology

authors： Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh C

更新日期：2014-11-28 00:00:00

abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...

journal_title：Journal of microbiology and biotechnology

authors： Kim HJ,Jung WK,Lee HW,Yoo W,Kim TD,Kim H

更新日期：2015-12-28 00:00:00

abstract：:Lactobacillus brevis ATCC 14869 exhibited a carbon catabolite de-repressed (CCR) phenotype which has ability to consume fermentable sugar simultaneously with glucose. To evaluate this unusual phenotype under harsh conditions during fermentation, the effect of lactic acid and hydrogen ion concentrations on L. brevis AT...

journal_title：Journal of microbiology and biotechnology

authors： Jeong KH,Israr B,Shoemaker SP,Mills DA,Kim J

更新日期：2016-07-28 00:00:00

abstract：:Five fusion proteins between Z domains derived from Staphylococcal Protein A and Green Fluorescent Protein or Human Proinsulin were produced on the periplasm of Escherichia coli. The effects of the molecular weight and amino acid composition of the translocated peptide, culture medium composition, and growth phase of ...

journal_title：Journal of microbiology and biotechnology

authors： Mergulhão FJ,Monteiro GA

更新日期：2007-08-01 00:00:00

abstract：:The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...

journal_title：Journal of microbiology and biotechnology

authors： Oh SW,Jang MU,Jeong CK,Kang HJ,Park JM,Kim TJ

更新日期：2008-08-01 00:00:00

abstract：:Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and...

journal_title：Journal of microbiology and biotechnology

authors： Kim YO,Park IS,Nam BH,Kim DG,Jee YJ,Lee SJ,An CM

更新日期：2014-09-01 00:00:00

abstract：:Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to 85 degrees C. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We chara...

journal_title：Journal of microbiology and biotechnology

authors： Lim YR,Eun CY,Park HG,Han S,Han JS,Cho KS,Chun YJ,Kim D

更新日期：2010-03-01 00:00:00

abstract：:Laccase- and peroxidase-free tyrosinase has commercial importance in the production of L-3, 4-dihydroxyphenylalanine (L-DOPA), which is mainly used in the treatment of Parkinson's disease. In the present study, isolation of an actinomycetes microbial strain capable of producing only tyrosinase is reported. Among all s...

journal_title：Journal of microbiology and biotechnology

authors： Sambasiva Rao KR,Tripathy NK,Mahalaxmi Y,Prakasham RS

更新日期：2012-02-01 00:00:00

abstract：:The SMG1 lipase from Malassezia globosa is a newly found mono- and diacylglycerol (DAG) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. In the present study, we characterized the contribution of three residues, L103 and F104 in the lid and F278 in the rim of the bind...

journal_title：Journal of microbiology and biotechnology

authors： Lan D,Wang Q,Popowicz GM,Yang B,Tang Q,Wang Y

更新日期：2015-11-01 00:00:00

abstract：:The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do ...

journal_title：Journal of microbiology and biotechnology

authors： Gomaa AE,Deng Z,Yang Z,Shang L,Zhan Y,Lu W,Lin M,Yan Y

更新日期：2017-02-28 00:00:00

abstract：:In this study, a method of heat adaptation was implemented in an attempt to increase the upper thermal threshold of two Streptococcus thermophilus found in South Korea and identified the alterations in membrane fatty acid composition to adaptive response to heat. In order to develop heat tolerant lactic acid bacteria,...

journal_title：Journal of microbiology and biotechnology

authors： Min B,Kim K,Li V,Cho S,Kim H

更新日期：2020-05-28 00:00:00

abstract：:Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus T...

journal_title：Journal of microbiology and biotechnology

authors： Jirasatid S,Nopharatana M,Kitsubun P,Vichitsoonthonkul T,Tongta A

更新日期：2013-03-01 00:00:00

abstract：:A denitrifying polyphosphate-accumulating bacterium (YKP-9) was isolated from activated sludge of a 5-stage biological nutrient removal process with step feed system. This organism was a Gram-negative, coccus-shaped, facultative aerobic chemoorganotroph. It had a respiratory type of metabolism with oxygen, nitrate, an...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Park YK

更新日期：2008-12-01 00:00:00

abstract：:Nargenicin A1 is a 28-membered polyketide macrolide, with antibacterial activity against methicillin-resistant Staphylococcus aureus, produced by Nocardia sp. CS682. In this study, the production of nargenicin A1 was improved by enhancing the supply of different biosynthetic precursors. In Nocardia sp. CS682 (KCTC1129...

journal_title：Journal of microbiology and biotechnology

authors： Koju D,Maharjan S,Dhakal D,Yoo JC,Sohng JK

更新日期：2012-08-01 00:00:00

abstract：:The pharmacokinetics of decursin and decursinol angelate (D/DA) was investigated in male SD rats following oral and intravenous administration. D/DA and metabolites obtained from in vitro samples were evaluated by LC/MS. The level of D/DA and metabolized decursinol in the blood following oral and intravenous administr...

journal_title：Journal of microbiology and biotechnology

authors： Kim KM,Kim MJ,Kang JS

更新日期：2009-12-01 00:00:00

abstract：:Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capabili...

journal_title：Journal of microbiology and biotechnology

authors： Wang C,Yu S,Song T,He T,Shao H,Wang H

更新日期：2016-11-28 00:00:00

abstract：:Ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate((S)-HEES)acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which canbe achieved viathe stereoselective bioreduction ofethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that containsa 3-oxoacyl structure.The sequences of the short-chain dehydroge...

journal_title：Journal of microbiology and biotechnology

authors： Ren ZQ,Liu Y,Pei XQ,Wu ZL

更新日期：2019-11-28 00:00:00

abstract：:The synergy effect of trisodium phosphate (TSP) and ethanol against murine norovirus 1 (MNV-1), as a surrogate for human noroviruses, on fresh produces was evaluated. More than 2% (w/v) of TSP effectively inactivated MNV-1. The single treatment of 1% TSP or 30% ethanol for 30 min was not effective on MNV-1; however, c...

journal_title：Journal of microbiology and biotechnology

authors： Kim EJ,Lee YD,Kim KY,Park JH

更新日期：2015-12-28 00:00:00