Screening, Gene Cloning, and Characterizations of an Acid-Stable α-Amylase.

abstract：:As an opportunistic bacterial pathogen, Pseudomonas aeruginosa PAO1 contains two phenazineproducing gene operons, phzA1B1C1D1E1F1G1 (phz1) and phzA2B2C2D2E2F2G2 (phz2), each of which is independently capable of encoding all enzymes for biosynthesizing phenazines, including phenazine-1-carboxylic acid and its derivativ...

journal_title：Journal of microbiology and biotechnology

authors： He Q,Feng Z,Wang Y,Wang K,Zhang K,Kai L,Hao X,Yu Z,Chen L,Ge Y

更新日期：2019-08-28 00:00:00

abstract：:The gut microbiome influences the health and well-being of dogs. However, little is known about the impact of breed on the fecal microbiome composition in dogs. Therefore, we aimed to investigate the differences in the fecal microbiome in three breeds of dog fed and housed under the same conditions, namely eight Malte...

journal_title：Journal of microbiology and biotechnology

authors： Reddy KE,Kim HR,Jeong JY,So KM,Lee S,Ji SY,Kim M,Lee HJ,Lee S,Kim KH,Kim M

更新日期：2019-12-28 00:00:00

abstract：:Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There ar...

journal_title：Journal of microbiology and biotechnology

authors： Kim S,Kim HE,Kang B,Lee YW,Kim H,Chung DK

更新日期：2017-10-28 00:00:00

abstract：:Enrofloxacin is a fluoroquinolone antibiotic approved for the treatment of infections in animals. Because of the side effects to consumers of animal products, the maximum residue limits (MRLs) of enrofloxacin in animal tissues for consumption are regulated. In this study, a monoclonal antibody (mAb) against enrofloxac...

journal_title：Journal of microbiology and biotechnology

authors： Chusri M,Wongphanit P,Palaga T,Puthong S,Sooksai S,Komolpis K

更新日期：2013-01-01 00:00:00

abstract：:The effect of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF4]), on the horseradish peroxidase (HRP)-catalyzed oxidation of 2-methoxyphenol (guaiacol) with hydrogen peroxide (H2O2) was investigated. HRP maintains its high activity in the aqueous mixtures containing various conc...

journal_title：Journal of microbiology and biotechnology

authors： Lee YM,Kwon OY,Yoo IK,Ryu KG

更新日期：2007-04-01 00:00:00

abstract：:Rumex japonicus Houtt (RJH) is a valuable plant used in traditional medicine to treat several diseases, such as scabies and jaundice. In this study, Jurkat cell growth inhibitory extracts of R. japonicus roots were subjected to bioassay-guided fractionation, resulting in the isolation of three naphthalene derivatives ...

journal_title：Journal of microbiology and biotechnology

authors： Qiu Y,Li A,Lee J,Lee JE,Lee EW,Cho N,Yoo HM

更新日期：2020-12-28 00:00:00

abstract：:The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNAdependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular ...

journal_title：Journal of microbiology and biotechnology

authors： Kim JH,Rhee JK,Ahn DG,Kim KP,Oh JW

更新日期：2014-12-28 00:00:00

abstract：:Two novel avian beta-defensins (AvBDs) isolated from duck liver were characterized and their homologies with other AvBDs were analyzed. They were shown to be duck AvBD9 and AvBD10. The mRNA expression of the two genes was analyzed in 17 different tissues from 1-28-dayold ducks. AvBD9 was differentially expressed in th...

journal_title：Journal of microbiology and biotechnology

authors： Ma D,Liao W,Wang R,Han Z,Liu S

更新日期：2009-11-01 00:00:00

abstract：:The purified endochitosanase (Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers (GlcN)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this e...

journal_title：Journal of microbiology and biotechnology

authors： Gao XA,Jung WJ,Kuk JH,Park RD

更新日期：2009-04-01 00:00:00

abstract：:This study examined the laxative effects of hot-water extracts of Hovenia dulcis Thunb. (HD), Phyllostachys pubescens Mazel (PM), and a 2:8 mixture of both (HP) in two chronic constipation models. For the loperamide-induced constipation model, animals were divided into an untreated group, negative control group (loper...

journal_title：Journal of microbiology and biotechnology

authors： Oh KN,Kim Y,Choi EJ,Lee H,Hong JA,Kim M,Oh DR,Jung MA,Park RD,Kim SI,Yong JS,Lee HS,Ban S,Choi CY

更新日期：2020-05-28 00:00:00

abstract：:A series of thiobarbituric acid derivatives were constructed and evaluated for inhibitory activity on hepatitis C virus NS5B polymerase. In biochemical assays using purified viral polymerase and RNA template, the IC(50) value was improved to 0.41 microM from the original compound's 1.7 microM value. In HCV subgenomic ...

journal_title：Journal of microbiology and biotechnology

authors： Lee JH,Lee S,Park MY,Ha HJ,Myung H

更新日期：2010-03-01 00:00:00

abstract：:The past decades have been a golden era during which great tasks were accomplished in the field of microbiology, including food microbiology. In the past, culture-dependent methods have been the primary choice to investigate bacterial diversity. However, using cultureindependent high-throughput sequencing of 16S rRNA ...

journal_title：Journal of microbiology and biotechnology

authors： Kim BR,Shin J,Guevarra R,Lee JH,Kim DW,Seol KH,Lee JH,Kim HB,Isaacson R

更新日期：2017-12-28 00:00:00

abstract：:The effects of two different sugars (glucose and xylose) on the expression levels and patterns of xylose reductase (xyl1), xylitol dehydrogenase (xyl2) and xylulokinase (xyl3) genes were analyzed using Pichia stipitis. A significant increase in mRNA levels of xyl1 was observed after 6 hours growth in culture condition...

journal_title：Journal of microbiology and biotechnology

authors： Han JH,Park JY,Kang HW,Choi GW,Chung BW,Min J

更新日期：2010-05-01 00:00:00

abstract：:This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Yin H,Xu X,Cheng Y,Cai Y,Wang R

更新日期：2015-10-01 00:00:00

abstract：:The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do ...

journal_title：Journal of microbiology and biotechnology

authors： Gomaa AE,Deng Z,Yang Z,Shang L,Zhan Y,Lu W,Lin M,Yan Y

更新日期：2017-02-28 00:00:00

abstract：:An antioxidant peptide derived from Pinctada fucata meat using an Alcalase2.4L enzymatic hydrolysis method (named AOP) and identified by LC-TOF-MS has promising clinical potential for generating cosmetic products that protect skin from sunshine. To date, there have been few published studies investigating the structur...

journal_title：Journal of microbiology and biotechnology

authors： Wu Y,Ma Y,Li L,Yang X

更新日期：2018-01-28 00:00:00

abstract：:Laccase- and peroxidase-free tyrosinase has commercial importance in the production of L-3, 4-dihydroxyphenylalanine (L-DOPA), which is mainly used in the treatment of Parkinson's disease. In the present study, isolation of an actinomycetes microbial strain capable of producing only tyrosinase is reported. Among all s...

journal_title：Journal of microbiology and biotechnology

authors： Sambasiva Rao KR,Tripathy NK,Mahalaxmi Y,Prakasham RS

更新日期：2012-02-01 00:00:00

abstract：:White spot syndrome virus (WSSV) is a major threat to the shrimp farming industry and so far there is no effective therapy for it, and thus early diagnostic of WSSV is of great importance. However, at the early stage of infection, the extremely low-abundance of WSSV DNA challenges the detection sensitivity and accurac...

journal_title：Journal of microbiology and biotechnology

authors： Pan X,Zhang Y,Sha X,Wang J,Li J,Dong P,Liang X

更新日期：2017-03-28 00:00:00

abstract：:Benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon, is a principal component of cigarette smoke. B[a]P can cause lung carcinogenesis and plays a key role in lung cancer progression. The role of B[a]P has been reported in lung cancer, but its effects on lung cancer stem cells (CSCs) have not been investigated. E...

journal_title：Journal of microbiology and biotechnology

authors： Bak Y,Jang HJ,Seo JH,No SH,Chae JI,Hong J,Yoon DY

更新日期：2018-03-28 00:00:00

abstract：:We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of ...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Hong J,Zhang P,Lu LF,Hwang JS,Park KC,Kim H

更新日期：2015-10-01 00:00:00

abstract：:Commercial application of laccase is often hampered by insufficient enzyme stocks, with very low yields obtained from natural sources. This study aimed to improve laccase production by mutation of a Coriolopsis gallica strain and to determine the biological properties of the mutant. The high-yield laccase strain C. ga...

journal_title：Journal of microbiology and biotechnology

authors： Xu X,Feng L,Han Z,Luo S,Wu A,Xie J

更新日期：2016-09-28 00:00:00

abstract：:A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA ...

journal_title：Journal of microbiology and biotechnology

authors： Rajkumar R,Kothilmozhian J,Ramasamy R

更新日期：2011-06-01 00:00:00

abstract：:Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were...

journal_title：Journal of microbiology and biotechnology

authors： Jin SD,Kim YM,Kang HK,Jung SJ,Kim D

更新日期：2009-11-01 00:00:00

abstract：:In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homo...

journal_title：Journal of microbiology and biotechnology

authors： Li SB,Xu SR,Zhang RN,Liu Y,Zhou RC

更新日期：2016-04-28 00:00:00

abstract：:This study compared the radioprotective effects of high-molecular-weight poly-gamma-glutamate (γ-PGA, average molecular mass 3,000 kDa) and a reduced form of glutathione (GSH, a known radioprotector) on calf thymus DNA damage. The radiation-induced DNA damage was measured on the basis of the decreased fluorescence int...

journal_title：Journal of microbiology and biotechnology

authors： Oh YJ,Kwak MS,Sung MH

更新日期：2018-04-28 00:00:00

abstract：:Fermentation with filamentous fungi in a bioreactor is a complex dynamic process that is affected by flow conditions and the evolution of the rheological properties of the medium. These properties are mainly affected by the biomass concentration and the morphology of the fungus. In this work, the rheological propertie...

journal_title：Journal of microbiology and biotechnology

authors： Núñez-Ramírez DM,Medina-Torres L,Valencia-López JJ,Calderas F,López Miranda J,Medrano-Roldán H,Solís-Soto A

更新日期：2012-11-01 00:00:00

abstract：:This study was undertaken to evaluate the immunomodulatory effect of dead nano-sized Lactobacillus plantarum (nLp) in RAW 264.7 cells and murine primary splenocytes. nLp is a dead, shrunken, processed form of L. plantarum nF1 isolated from kimchi (a traditional Korean fermented cabbage) and is less than 1 μm in size. ...

journal_title：Journal of microbiology and biotechnology

authors： Lee HA,Kim H,Lee KW,Park KY

更新日期：2016-03-01 00:00:00

abstract：:The synergy effect of trisodium phosphate (TSP) and ethanol against murine norovirus 1 (MNV-1), as a surrogate for human noroviruses, on fresh produces was evaluated. More than 2% (w/v) of TSP effectively inactivated MNV-1. The single treatment of 1% TSP or 30% ethanol for 30 min was not effective on MNV-1; however, c...

journal_title：Journal of microbiology and biotechnology

authors： Kim EJ,Lee YD,Kim KY,Park JH

更新日期：2015-12-28 00:00:00

abstract：:Inactivation of Escherichia coli O157:H7 and Salmonella typhimurium was evaluated on inoculated chicken by aqueous chlorine dioxide (ClO2) treatment. Chicken samples were inoculated with 6-7 log CFU/g of Escherichia coli O157:H7 and Salmonella typhimurium, respectively. The chicken samples were then treated with 0, 50...

journal_title：Journal of microbiology and biotechnology

authors： Hong Y,Ku K,Kim M,Won M,Chung K,Song KB

更新日期：2008-04-01 00:00:00

abstract：:Leaves are the major biomass-producing organs in herbaceous plants and mainly develop during vegetative stage by activities of shoot apical meristem. There is a strong correlation between leaf number and bolting, a characteristic phenotype during the transition to reproductive phase in Arabidopsis thaliana. In order t...

journal_title：Journal of microbiology and biotechnology

authors： Lee BH

更新日期：2009-06-01 00:00:00