Abstract：
:Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, there was not much information on antibiotic resistance of Halomonas species. As an effort to find antibiotic resistance of Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics, including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin was different in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions, and by performing time dependent reverse transcription PCR. We found that aminoglycoside phosphotransferase (aph2) showed increased expression under the 10% than 1% NaCl conditions. When these genes were overexpressed in an Escherichia coli strain, pETDuet-1::aph2 showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control suggesting aph2 was involved in aminoglycoside resistance. Our results showed a more direct link between NaCl and aminoglycoside resistance exhibited by the H. socia CKY01 strain.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Park YL,Choi TR,Kim HJ,Song HS,Lee HS,Park SL,Lee SM,Kim SH,Park S,Bhatia SK,Gurav R,Sung C,Seo SO,Yang YHdoi
10.4014/jmb.2009.09017subject
Has Abstractpub_date
2020-11-04 00:00:00eissn
1017-7825issn
1738-8872pii
jmb.2009.09017pub_type
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