Morphological changes induced in listeria monocytogenes V7 by a bacteriocin produced by Pediococcus acidilactici.

Abstract:

:Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. The bacteriocin (6,400 AU/ ml) was inoculated with L. monocytogenes V7 and incubated for 0.5 h, 1 h, 3 h, and 6 h. The bacteriocin was found to destroy most of the cell wall and released most of the inclusions in the cells after 6 h of incubation. These results suggest that the bactericidal effect of the bacteriocin was due to bacterial lysis.

journal_name

J Microbiol Biotechnol

authors

Heo S,Lee SK,Lee CH,Min SG,Park JS,Kim HY

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

663-7

issue

4

eissn

1017-7825

issn

1738-8872

journal_volume

17

pub_type

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