Abstract：
:Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its control. Therefore, we performed a differential proteome mapping strategy using two-dimensional gel electrophoresis combined with peptide mass fingerprinting to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3-L1 pre-adipocytes in response to an adipogenic cocktail. In the current study, we identified 46 differentially expressed proteins, 6 of which have not been addressed previously in 3T3-L1 cell differentiation. Notably, we found that phosphoribosyl pyrophosphate synthetase (PRPS), a regulator of cell proliferation, was preferentially expressed in pre-adipocytes than in fully differentiated adipocytes. In conclusion, our results provide valuable information for further understanding of the adipogenic process.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Atiar Rahman M,Kumar SG,Lee SH,Hwang HS,Kim HA,Yun JWsubject
Has Abstractpub_date
2008-12-01 00:00:00pages
1895-902issue
12eissn
1017-7825issn
1738-8872pii
8151journal_volume
18pub_type
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journal_title：Journal of microbiology and biotechnology
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更新日期：2008-10-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章
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更新日期：2013-02-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
pub_type： 杂志文章,评审
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journal_title：Journal of microbiology and biotechnology
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更新日期：2010-03-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-02-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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journal_title：Journal of microbiology and biotechnology
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更新日期：2007-01-01 00:00:00
abstract：:A Gram-positive and endospore-forming strain, JH8T, was isolated from deep-sea sediment and identified as a member of the genus Paenibacillus on the basis of 16S rRNA gene sequence and phenotypic analyses. According to a phylogenetic analysis, the most closely related species was Paenibacillus wynnii LMG 22176T (96.9%...
journal_title：Journal of microbiology and biotechnology
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更新日期：2008-02-01 00:00:00
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journal_title：Journal of microbiology and biotechnology
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doi：10.4014/jmb.1305.05070
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abstract：:A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...
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