Genetic characterization of two putative toxin-antitoxin systems on cryptic plasmids from Bacillus thuringiensis strain YBT-1520.

abstract：:Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...

journal_title：Journal of microbiology and biotechnology

authors： Lee JK,Shin OS

更新日期：2020-12-31 00:00:00

abstract：:With emphasis on thermal behavior in presence of different pH conditions and salts, the kinetic and thermodynamic parameters of purified polygalacturonase (PG) of E. carotovora subsp. carotovora (Ecc) BR1 were studied since characterization of an enzyme is significant in the context of burgeoning biotechnological appl...

journal_title：Journal of microbiology and biotechnology

authors： Maisuria VB,Patel VA,Nerurkar AS

更新日期：2010-07-01 00:00:00

abstract：:Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...

journal_title：Journal of microbiology and biotechnology

authors： Kim JJ,Kwon YK,Kim JH,Heo SJ,Lee Y,Lee SJ,Shim WB,Jung WK,Hyun JH,Kwon KK,Kang DH,Oh C

更新日期：2014-11-28 00:00:00

abstract：:Streptococcus suis serotype 2 (SS2), considered to be the most frequent and virulent type among identified serotypes, is an important emerging or re-emerging zoonotic pathogen. Inflammation is thought to be a hallmark of SS2 infection, as demonstrated by most clinical symptoms varying from an asymptomatic bacteremia t...

journal_title：Journal of microbiology and biotechnology

authors： Shen X,Li G,Deng X,Wang J,Zhang P

更新日期：2019-04-22 00:00:00

abstract：:The present study shows that DR1114 (Hsp20), a small heat shock protein of the radiationresistant bacterium Deinococcus radiodurans, enhances tolerance to hydrogen peroxide (H2O2) stress when expressed in Escherichia coli. A protein profile comparison showed that E. coli cells overexpressing D. radiodurans Hsp20 (EC-p...

journal_title：Journal of microbiology and biotechnology

authors： Singh H,Appukuttan D,Lim S

更新日期：2014-08-01 00:00:00

abstract：:The emergence of microcarrier technology has brought a renewed interest in anchorage-dependent cell culture for high-yield processes. Well-known in vaccine production, microcarrier culture also has potential for application in other fields. In this work, two types of microcarriers were evaluated for small-scale monocl...

journal_title：Journal of microbiology and biotechnology

authors： Rodrigues ME,Costa AR,Fernandes P,Henriques M,Cunnah P,Melton DW,Azeredo J,Oliveira R

更新日期：2013-09-28 00:00:00

abstract：:Mussels are major fouling organisms causing serious technical and economic problems. In this study, antifouling activity towards mussel was found in three compounds isolated from a marine bacterium associated with the sea anemone Haliplanella sp. This bacterial strain, called PE2, was identified as Vibrio alginolyticu...

journal_title：Journal of microbiology and biotechnology

authors： Wang X,Huang Y,Sheng Y,Su P,Qiu Y,Ke C,Feng D

更新日期：2017-03-28 00:00:00

abstract：:Diarrhea is a global disease with a high morbidity and mortality rate in children. In this study, 25 fecal samples were collected from children under 5 years old. Seven samples had been taken from healthy children without diarrhea and marked as the healthy control group; eight samples had been sampled from children wi...

journal_title：Journal of microbiology and biotechnology

authors： Wen H,Yin X,Yuan Z,Wang X,Su S

更新日期：2018-04-28 00:00:00

abstract：:In this study, we developed lysosome-alginate beads for application as an oral drug delivery system (ODDS). The beads harboring lysosomes, which have antimicrobial activity, and various concentrations of alginate were characterized and optimized. For application as an ODDS, pH-dependent lysosome-alginate beads were ge...

journal_title：Journal of microbiology and biotechnology

authors： Park HJ,Min J,Ahn JM,Cho SJ,Ahn JY,Kim YH

更新日期：2015-02-01 00:00:00

abstract：:Thirty-seven carbofuran-degrading bacteria were isolated from agricultural soils, and their genetic and phenotypic characteristics were investigated. The isolates were able to utilize carbofuran as a sole source of carbon and energy. Analysis of the 16S rRNA gene sequence indicated that the isolates were related to me...

journal_title：Journal of microbiology and biotechnology

authors： Shin DH,Kim DU,Seong CN,Song HG,Ka JO

更新日期：2012-04-01 00:00:00

abstract：:An intracellular lipase from Anoxybacillus flavithermus HBB 134 was purified to 7.4-fold. The molecular mass of the enzyme was found to be about 64 kDa. The maximum activity of the enzyme was at pH 9.0 and 50°C. The enzyme was stable between pH 6.0 and 11.0 at 25°C, 40°C, and 50°C for 24 h. The Km and Vmax of the enzy...

journal_title：Journal of microbiology and biotechnology

authors： Bakir ZB,Metin K

更新日期：2016-06-28 00:00:00

abstract：:Biologically produced 2,3-butanediol (2,3-BDO) has diverse industrial applications. In this study, schematic isolation and screening procedures were designed to obtain generally regarded as safe (GRAS) and efficient 2,3-BDO producers. Over 4,000 candidate strains were isolated by pretreatment and enrichment, and the i...

journal_title：Journal of microbiology and biotechnology

authors： Song CW,Rathnasingh C,Park JM,Lee J,Song H

更新日期：2018-03-28 00:00:00

abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...

journal_title：Journal of microbiology and biotechnology

authors： Zheng CJ,Sohn MJ,Chi SW,Kim WG

更新日期：2010-05-01 00:00:00

abstract：:Bacillus pumilus is one of the most characterized microorganisms that are used for high-level production of select industrial enzymes. A novel B. pumilus SCU11 strain possessing high alkaline protease activity was obtained in our previous work. The culture supernatant of this strain showed efficient dehairing capabili...

journal_title：Journal of microbiology and biotechnology

authors： Wang C,Yu S,Song T,He T,Shao H,Wang H

更新日期：2016-11-28 00:00:00

abstract：:A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell g...

journal_title：Journal of microbiology and biotechnology

authors： Rho JK,Choi MH,Shim JH,Lee SY,Woo MJ,Ko BS,Chi KW,Yoon SC

更新日期：2007-12-01 00:00:00

abstract：:It has been well known that the use of Saccharomyces cerevisiae can cause fungemia in critically ill patients and flavone shows an antimicrobial effect on S. cerevisiae. Therefore, we have investigated the activities of thirteen flavone analogues on S. cerevisiae in our studies. Because flavonoids including flavones h...

journal_title：Journal of microbiology and biotechnology

authors： Young J,Park Y,Lee YU,Kim H,Shim YH,Ahn JH,Lim Y

更新日期：2007-03-01 00:00:00

abstract：:Extremophilic microorganisms have established a diversity of molecular strategies in order to survive in extreme conditions. Biocatalysts isolated by these organisms are termed extremozymes, and possess extraordinary properties of salt allowance, thermostability, and cold adaptivity. Extremozymes are very resistant to...

journal_title：Journal of microbiology and biotechnology

authors： Dumorné K,Córdova DC,Astorga-Eló M,Renganathan P

更新日期：2017-04-28 00:00:00

abstract：:A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 ...

journal_title：Journal of microbiology and biotechnology

authors： Wen C,Guo W,Chen X

更新日期：2014-10-01 00:00:00

abstract：:Monoamine oxidase (MAO) is found in most cell types and catalyzes the oxidation of monoamines. Three anithiactins (A-C, modified 2-phenylthiazoles) isolated from Streptomyces sp. were tested for inhibitory activity of two isoforms, MAO-A and MAO-B. Anithiactin A was effective and selective for the inhibition of MAO-A,...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Jung WK,Kim HJ,Jeong YS,Nam SJ,Kang H,Kim H

更新日期：2015-09-01 00:00:00

abstract：:Cameleon is a genetically engineered Ca2+ sensing molecule consisting of two variants of the green fluorescent protein (GFP), calmodulin and calmodulin-binding protein, M13. HEK-293 cells stably expressing three types of cameleons, yellow cameleon-2, cameleon-3er, and cameleon-2nu, were constructed, and the expression...

journal_title：Journal of microbiology and biotechnology

authors： Ko HJ,Park TH

更新日期：2007-06-01 00:00:00

abstract：:An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60 degrees C and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and ...

journal_title：Journal of microbiology and biotechnology

authors： Chimtong S,Tachaapaikoon C,Pason P,Kyu KL,Kosugi A,Mori Y,Ratanakhanokchai K

更新日期：2011-03-01 00:00:00

abstract：:Glucosyl rubusosides were synthesized by two dextransucrases. LcDexT was obtained from Leuconosotoc citreum, that LlDexT was obtained from Leuconostoc lactis. LcDexT and LlDexT regioselectively transferred a glucosyl residue to the 13-O-glucosyl moiety of rubusoside with high yield of 59-66% as analyzed by TLC and HPL...

journal_title：Journal of microbiology and biotechnology

authors： Ko JA,Ryu YB,Park JY,Kim CY,Kim JS,Nam SH,Lee WS,Kim YM

更新日期：2016-03-01 00:00:00

abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...

journal_title：Journal of microbiology and biotechnology

authors： Wang J,Wang ZY,He XP,Zhang BR

更新日期：2010-11-01 00:00:00

abstract：:The identification of bacterial pathogens to humans is critical for environmental microbial risk assessment. However, current methods for identifying pathogens in environmental samples are limited in their ability to detect highly diverse bacterial communities and accurately differentiate pathogens from commensal bact...

journal_title：Journal of microbiology and biotechnology

authors： Yang J,Howe A,Lee J,Yoo K,Park J

更新日期：2020-09-28 00:00:00

abstract：:The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...

journal_title：Journal of microbiology and biotechnology

authors： Oh SW,Jang MU,Jeong CK,Kang HJ,Park JM,Kim TJ

更新日期：2008-08-01 00:00:00

abstract：:The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen ty...

journal_title：Journal of microbiology and biotechnology

authors： Gil CS,Chang MS,Eom SH

更新日期：2017-08-28 00:00:00

abstract：:Codium fragile is an edible seaweed in Asian countries that has been used as a thrombolytic, anticoagulant, antioxidant, anti-inflammatory, and immune-stimulatory agent. Ginseng has also been known to maintain immune homeostasis and to regulate the immune system via enhancing resistance to diseases and microorganisms....

journal_title：Journal of microbiology and biotechnology

authors： Kim JE,Monmai C,Rod-In W,Jang AY,You SG,Lee SM,Park WJ

更新日期：2019-09-28 00:00:00

abstract：:Ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate((S)-HEES)acts as a key chiral intermediate for the blockbuster antidepressant drug duloxetine, which canbe achieved viathe stereoselective bioreduction ofethyl 3-oxo-3-(2-thienyl) propanoate (KEES) that containsa 3-oxoacyl structure.The sequences of the short-chain dehydroge...

journal_title：Journal of microbiology and biotechnology

authors： Ren ZQ,Liu Y,Pei XQ,Wu ZL

更新日期：2019-11-28 00:00:00

abstract：:A Myagropsis myagroides (Mm) methanol extract showed α-amylase inhibitory activity of 13% at a concentration of 5 mg/ml. Results showed that the hexane fraction from the Mm methanol extract exhibited α-amylase inhibitory activity with an IC50 value of 4.24 mg/ml. The hexane fraction was separated using silica-gel colu...

journal_title：Journal of microbiology and biotechnology

authors： Pak WM,Kim KB,Kim MJ,Cho JY,Ahn DH

更新日期：2015-03-01 00:00:00

abstract：:The impact of overproduction of a heterologous protein on the metabolic system of host Lactococcus lactis was investigated. The protein expression profiles of L. lactis IL1403 containing two near-identical plasmids that expressed high- and low-level of the green fluorescent protein (GFP) were examined via shotgun prot...

journal_title：Journal of microbiology and biotechnology

authors： Kim M,Jin Y,An HJ,Kim J

更新日期：2017-07-28 00:00:00