Comparison of Microbial Diversity and Composition in Jejunum and Colon of the Alcohol-dependent Rats.

abstract：:The pharmacokinetics of decursin and decursinol angelate (D/DA) was investigated in male SD rats following oral and intravenous administration. D/DA and metabolites obtained from in vitro samples were evaluated by LC/MS. The level of D/DA and metabolized decursinol in the blood following oral and intravenous administr...

journal_title：Journal of microbiology and biotechnology

authors： Kim KM,Kim MJ,Kang JS

更新日期：2009-12-01 00:00:00

abstract：:Conessine, a steroidal alkaloid, is a potent histamine H3 antagonist with antimalarial activity. We recently reported that conessine treatment interferes with H₂O₂-induced cell death by regulating autophagy. However, the cellular signaling pathways involved in conessine treatment are not fully understood. Here, we rep...

journal_title：Journal of microbiology and biotechnology

authors： Kim H,Jang M,Park R,Jo D,Choi I,Choe J,Oh WK,Park J

更新日期：2018-04-28 00:00:00

abstract：:A novel cry2Ab gene was cloned and sequenced from the indigenous isolate of Bacillus thuringiensis subsp. kurstaki. This gene was designated as cry2Ab25 and its sequence revealed an open reading frame of 1,902 bp encoding a 633 aa protein with calculated molecular mass of 70 kDa and pI value of 8.98. The amino acid se...

journal_title：Journal of microbiology and biotechnology

authors： Sevim A,Eryüzlü E,Demirbağ Z,Demir I

更新日期：2012-01-01 00:00:00

abstract：:The aim of this study was to isolate antioxidant peptides from an enzymatic hydrolysate of Spirulina platensis. A novel antioxidant peptide was obtained by ultrafiltration, gel filtration chromatography, and reverse-phase high-performance liquid chromatography, with the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical sca...

journal_title：Journal of microbiology and biotechnology

authors： Yu J,Hu Y,Xue M,Dun Y,Li S,Peng N,Liang Y,Zhao S

更新日期：2016-07-28 00:00:00

abstract：:Two new glucosides (1 and 2) of geldanamycin (GA) analogs were obtained from in vitro glycosylation by UDP-glycosyltransferase (YjiC). Based on spectroscopic (HR-ESI-MS, 1D, and 2D-NMR) analyses, the glucosides were elucidated as 4,5-dihydro-7-O-descarbamoyl-7- hydroxyl GA-7-O-β-D-glucoside (1) and ACDL3172-18-O-β-D-g...

journal_title：Journal of microbiology and biotechnology

authors： Huo Q,Li HM,Lee JK,Li J,Ma T,Zhang X,Dai Y,Hong YS,Wu CZ

更新日期：2016-01-01 00:00:00

abstract：:Plasmid isolation of kimchi-derived Weissella cibaria KLC140 revealed six different plasmids. The smallest plasmid, pKW2124, was DNA sequenced and characterized, showing 2,124 bp with a GC content of 36.39% and five putative open reading frames (ORFs). In silico analysis of these ORFs showed ORF1 encodes a putative re...

journal_title：Journal of microbiology and biotechnology

authors： Kim SY,Oh CG,Lee YJ,Choi KH,Shin DS,Lee SK,Park KJ,Shin H,Park MS,Lee JH

更新日期：2013-04-01 00:00:00

abstract：:Synthetic biology builds programmed biological systems for a wide range of purposes such as improving human health, remedying the environment, and boosting the production of valuable chemical substances. In recent years, the rapid development of synthetic biology has enabled synthetic bacterium-based diagnoses and the...

journal_title：Journal of microbiology and biotechnology

authors： Vo P,Lee HM,Na D

更新日期：2019-06-28 00:00:00

abstract：:Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity ...

journal_title：Journal of microbiology and biotechnology

authors： Lin X,Liu S,Xie G,Chen J,Li P,Chen J

更新日期：2016-11-28 00:00:00

abstract：:Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter is...

journal_title：Journal of microbiology and biotechnology

authors： Shin E,Lee Y

更新日期：2009-07-01 00:00:00

abstract：:Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from Paeonia lactiflora against Candida albicans, showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabli...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim Y

更新日期：2017-02-28 00:00:00

abstract：:During the fermentative production of 1,3-propanediol under high substrate concentrations, accumulation of intracellular 3-hydroxypropionaldehyde will cause premature cessation of cell growth and glycerol consumption. Discovery of oxidoreductases that can convert 3- hydroxypropionaldehyde to 1,3-propanediol using NADP...

journal_title：Journal of microbiology and biotechnology

authors： Ma CW,Zhang L,Dai JY,Xiu ZL

更新日期：2013-12-01 00:00:00

abstract：:Cameleon is a genetically engineered Ca2+ sensing molecule consisting of two variants of the green fluorescent protein (GFP), calmodulin and calmodulin-binding protein, M13. HEK-293 cells stably expressing three types of cameleons, yellow cameleon-2, cameleon-3er, and cameleon-2nu, were constructed, and the expression...

journal_title：Journal of microbiology and biotechnology

authors： Ko HJ,Park TH

更新日期：2007-06-01 00:00:00

abstract：:An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protei...

journal_title：Journal of microbiology and biotechnology

authors： Zhao S,Zhao Y,Li S,Zhang G,Wang H,Ng TB

更新日期：2010-04-01 00:00:00

abstract：:Streptococcus suis serotype 2 (SS2), considered to be the most frequent and virulent type among identified serotypes, is an important emerging or re-emerging zoonotic pathogen. Inflammation is thought to be a hallmark of SS2 infection, as demonstrated by most clinical symptoms varying from an asymptomatic bacteremia t...

journal_title：Journal of microbiology and biotechnology

authors： Shen X,Li G,Deng X,Wang J,Zhang P

更新日期：2019-04-22 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the r...

journal_title：Journal of microbiology and biotechnology

authors： Kim DG,Ahn SH,Kim LH,Park KJ,Hong YK,Kong IS

更新日期：2008-11-01 00:00:00

abstract：:The bioactivities of boxthron fruits, a source of oriental medicine, are well known, whereas phytochemical studies of the boxthorn stem are rare. In this study, the stem extract of boxthorn (Lycium chinense Miller) and its subfractions were evaluated for their effects on nitric oxide (NO) inhibition and procollagen ty...

journal_title：Journal of microbiology and biotechnology

authors： Gil CS,Chang MS,Eom SH

更新日期：2017-08-28 00:00:00

abstract：:Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is hi...

journal_title：Journal of microbiology and biotechnology

authors： Choi IY,Lee JH,Kim HJ,Park MK

更新日期：2017-12-28 00:00:00

abstract：:This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Yin H,Xu X,Cheng Y,Cai Y,Wang R

更新日期：2015-10-01 00:00:00

abstract：:Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransu...

journal_title：Journal of microbiology and biotechnology

authors： Lee MS,Cho SK,Eom HJ,Kim SY,Kim TJ,Han NS

更新日期：2008-06-01 00:00:00

abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...

journal_title：Journal of microbiology and biotechnology

authors： Wang J,Wang ZY,He XP,Zhang BR

更新日期：2010-11-01 00:00:00

abstract：:Controlling the residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (RC/N) of glucose and ammonia ...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Lee HS,Kim CS,Lee JG,Kim WK,Lee EG,Lee HW

更新日期：2018-02-28 00:00:00

abstract：:Doenjang, a traditional Korean fermented soybean paste, is made by mixing and ripening meju with high salt brine (approximately 18%). Meju is a naturally fermented soybean block prepared by soaking, steaming, and molding soybean. To understand living bacterial community migration and the roles of bacteria in the manuf...

journal_title：Journal of microbiology and biotechnology

authors： Jeong DW,Kim HR,Jung G,Han S,Kim CT,Lee JH

更新日期：2014-05-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthase is a key enzyme for PHA production in microorganisms. The class IV PHA synthase is composed of two subunits: PhaC and PhaR. The PhaR subunit, which encodes the phaR gene, is only present in class IV PHA synthases. Therefore, the phaR gene is used as a biomarker for bacteria that con...

journal_title：Journal of microbiology and biotechnology

authors： Wint NY,Han KK,Yamprayoonswat W,Ruangsuj P,Mangmool S,Promptmas C,Yasawong M

更新日期：2019-01-28 00:00:00

abstract：:Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus T...

journal_title：Journal of microbiology and biotechnology

authors： Jirasatid S,Nopharatana M,Kitsubun P,Vichitsoonthonkul T,Tongta A

更新日期：2013-03-01 00:00:00

abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...

journal_title：Journal of microbiology and biotechnology

authors： Lim HD,Cheong DE,Shin HJ,Kim GJ

更新日期：2010-11-01 00:00:00

abstract：:We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of ...

journal_title：Journal of microbiology and biotechnology

authors： Kim DH,Lee IH,Nam ST,Hong J,Zhang P,Lu LF,Hwang JS,Park KC,Kim H

更新日期：2015-10-01 00:00:00

abstract：:Keratinases are exciting keratin-degrading enzymes; however, there have been relatively few studies on their immobilization. A keratinolytic protease from Chryseobacterium sp. kr6 was purified and its partial sequence determined using mass spectrometry. No significant homology to other microbial peptides in the NCBI d...

journal_title：Journal of microbiology and biotechnology

authors： Silveira ST,Gemelli S,Segalin J,Brandellli A

更新日期：2012-06-01 00:00:00

abstract：:The time-consuming and high-cost preparation of soluble peptide-major histocompatibility complexes (pMHC) currently limits their wide uses in monitoring antigen-specific T cells. The single-chain trimer (SCT) of peptide-β2m-MHC class I heavy chain was developed as an alternative strategy, but its gene fusion is hinder...

journal_title：Journal of microbiology and biotechnology

authors： Xu T,Li X,Wu Y,Shahzad KA,Wang W,Zhang L,Shen C

更新日期：2016-12-28 00:00:00

abstract：:Bacterial heme was produced from a genetic-engineered Escherichia coli via the porphyrin pathway and it was useful as an iron resource for animal feed. The amount of the E. colisynthesized heme, however, was only few milligrams in a culture broth and it was not enough for industrial applications. To analyze heme biosy...

journal_title：Journal of microbiology and biotechnology

authors： Pranawidjaja S,Choi SI,Lay BW,Kim P

更新日期：2015-06-01 00:00:00

abstract：:Mixotrophic microalgal growth gives a great premise for wastewater treatment based on photoautotrophic nutrient utilization and heterotrophic organic removal while producing renewable biomass. There remains a need for a control strategy to enrich them in a photobioreactor. This study performed a series of batch experi...

journal_title：Journal of microbiology and biotechnology

authors： Lee TH,Jang JK,Kim HW

更新日期：2017-11-28 00:00:00