Biodegradation of di-n-butyl phthalate by Rhodococcus sp. JDC-11 and molecular detection of 3, 4-phthalate dioxygenase gene.

Abstract:

:Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 was 8.0, 30 degrees C, and 175 rpm, respectively. In addition, the effect of glucose concentration on DBP degradation indicated that low concentration of glucose inhibited the degradation of DBP while high concentrations of glucose increased its degradation. Meanwhile, the substrates utilization test showed that JDC-11 could also utilize other phthalates. Furthermore, the major metabolites of DBP degradation were identified as mono-butyl phthalate and phthalic acid by gas chromatography-mass spectrometry and the metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11 was tentatively speculated. Using a set of new degenerate primer, partial sequence of the 3, 4-phthalate dioxygenase gene was obtained from the strain. Sequence analysis revealed that the phthalate dioxygenase gene of JDC-11 was highly homologous to the large subunit of phthalate dioxygenase from Rhodococcus coprophilus strain G9.

journal_name

J Microbiol Biotechnol

authors

Jin DC,Liang RX,Dai QY,Zhang RY,Wu XL,Chao WL

doi

10.4014/jmb.1004.04034

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

1440-5

issue

10

eissn

1017-7825

issn

1738-8872

pii

JMB020-10-11

journal_volume

20

pub_type

杂志文章
  • Laccase- and peroxidase-free tyrosinase production by isolated microbial strain.

    abstract::Laccase- and peroxidase-free tyrosinase has commercial importance in the production of L-3, 4-dihydroxyphenylalanine (L-DOPA), which is mainly used in the treatment of Parkinson's disease. In the present study, isolation of an actinomycetes microbial strain capable of producing only tyrosinase is reported. Among all s...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1106.06031

    authors: Sambasiva Rao KR,Tripathy NK,Mahalaxmi Y,Prakasham RS

    更新日期:2012-02-01 00:00:00

  • Characterization of Dye Decolorization in Cell-Free Culture Broth of Trametes versicolor CBR43.

    abstract::The dye decolorization rate in a cell-free culture broth of the white-rot fungus Trametes versicolor CBR43 was studied, including the effects of inhibitors of NaCl, Zn(II), and Cd(II) on dye decolorization activity. The maximum rates of dye decolorization in cell-free culture broth were 1,410, 44.7, 41.2, and 0.19 μmo...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1608.08008

    authors: Ryu H,Ryu HW,Cho KS

    更新日期:2017-01-28 00:00:00

  • Intermolecular interaction between Cry2Aa and Cyt1Aa and its effect on larvicidal activity against Culex quinquefasciatus.

    abstract::The Cyt1Aa protein of Bacillus thuringiensis susbp. israelensis elaborates demonstrable toxicity to mosquito larvae, but more importantly, it enhances the larvicidal activity of this species Cry proteins (Cry11Aa, Cry4Aa, and Cry4Ba) and delays the phenotypic expression of resistance to these that has evolved in Culex...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1301.01062

    authors: Bideshi DK,Waldrop G,Fernandez-Luna MT,Diaz-Mendoza M,Wirth MC,Johnson JJ,Park HW,Federici BA

    更新日期:2013-08-01 00:00:00

  • Kinetic studies of alkaline protease from Bacillus licheniformis NCIM-2042.

    abstract::An extensive investigation was carried out to describe the kinetics of cell growth, substrate consumption, and product formation in the batch fermentation using starch as substrate. Evaluation of intrinsic kinetic parameters was carried out using a best-fit unstructured model. A nonlinear regression technique was appl...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1206.06015

    authors: Bhunia B,Basak B,Bhattacharya P,Dey A

    更新日期:2012-12-01 00:00:00

  • Sustainable Production of Dihydroxybenzene Glucosides Using Immobilized Amylosucrase from Deinococcus geothermalis.

    abstract::The amylosucrase encoding gene from Deinococcus geothermalis DSM 11300 (DgAS) was codon-optimized and expressed in Escherichia coli. The enzyme was employed for biosynthesis of three different dihydroxybenzene glucosides using sucrose as the source of glucose moiety. The reaction parameters, including temperature, pH,...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1805.05054

    authors: Lee HS,Kim TS,Parajuli P,Pandey RP,Sohng JK

    更新日期:2018-09-28 00:00:00

  • Antagonistic potentiality of Trichoderma harzianum towards seed-borne fungal pathogens of winter wheat cv. Protiva in vitro and in vivo.

    abstract::The antagonistic effect of Trichoderma harzianum on a range of seed-borne fungal pathogens of wheat (viz. Fusarium graminearum, Bipolaris sorokiniana, Aspergillus spp., and Penicillium spp.) was assessed. The potential of T. harzianum as a biocontrol agent was tested in vitro and under field conditions. Coculture of t...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1107.07063

    authors: Hasan MM,Rahman SM,Kim GH,Abdallah E,Oh DH

    更新日期:2012-05-01 00:00:00

  • Pinus Densiflora Bark Extract (PineXol) Decreases Adiposity in Mice by Down-Regulation of Hepatic De Novo Lipogenesis and Adipogenesis in White Adipose Tissue.

    abstract::PineXol, extracted from Korean red pine bark, has beneficial effects, such as antioxidant, antiinflammatory, and antilipogenic activities in vitro. We tested the hypothesis that PineXol supplementation could have anti-obesity effects on mice fed a high-fat diet (HFD). Four-week-old male C57BL/6 mice were fed normal ch...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1612.12037

    authors: Ahn H,Go GW

    更新日期:2017-04-28 00:00:00

  • Metagenome resource for D-serine utilization in a DsdA-disrupted Escherichia coli.

    abstract::To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Lim MY,Lee HJ,Kim P

    更新日期:2011-04-01 00:00:00

  • Antimicrobial Peptide, Lumbricusin, Ameliorates Motor Dysfunction and Dopaminergic Neurodegeneration in a Mouse Model of Parkinson's Disease.

    abstract::We recently reported that the antimicrobial peptide Lumbricusin (NH2-RNRRWCIDQQA), isolated from the earthworm, increases cell proliferation in neuroblastoma SH-SY5Y cells. Here, we investigated whether Lumbricusin has neurotropic activity in mouse neural stem cells (MNSCs) and a protective effect in a mouse model of ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1507.07011

    authors: Kim DH,Lee IH,Nam ST,Hong J,Zhang P,Lu LF,Hwang JS,Park KC,Kim H

    更新日期:2015-10-01 00:00:00

  • Lipoteichoic Acid Isolated from Staphylococcus aureus Induces Both Epithelial-Mesenchymal Transition and Wound Healing in HaCaT Cells.

    abstract::Lipoteichoic acid (LTA), a cell wall component of gram-positive bacteria, is recognized by Toll-like receptor 2, expressed on certain mammalian cell surfaces, initiating signaling cascades that include nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinase. There ar...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1703.03068

    authors: Kim S,Kim HE,Kang B,Lee YW,Kim H,Chung DK

    更新日期:2017-10-28 00:00:00

  • Mitotic cohesin subunit Mcd1 regulates the progression of meiotic recombination in budding yeast.

    abstract::The cohesin complex holds sister chromatids together and prevents premature chromosome segregation until the onset of anaphase. Mcd1 (also known as Scc1), the α-kleisin subunit of cohesin, is a key regulatory subunit of the mitotic cohesin complex and is required for maintaining sister chromatid cohesion, chromosome o...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1501.01081

    authors: Lee MS,Yoon SW,Kim KP

    更新日期:2015-05-01 00:00:00

  • NaCl concentration-dependent aminoglycoside resistance of Halomonas socia CKY01 and identification of related genes.

    abstract::Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, there was not much information on antibiotic resistance of Halomonas species. As an effort to find antibiotic resistance of Halomonas socia CKY01, which showed produ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.2009.09017

    authors: Park YL,Choi TR,Kim HJ,Song HS,Lee HS,Park SL,Lee SM,Kim SH,Park S,Bhatia SK,Gurav R,Sung C,Seo SO,Yang YH

    更新日期:2020-11-04 00:00:00

  • Bifunctional recombinant fusion enzyme between maltooligosyltrehalose synthase and maltooligosyltrehalose trehalohydrolase of thermophilic microorganism Metallosphaera hakonensis.

    abstract::MhMTS and MhMTH are trehalose (alpha-D-glucopyranosyl- [1,1]-alpha-D-glucopyranose) biosynthesis genes of the thermophilic microorganism Metallosphaera hakonensis, and encode a maltooligosyltrehalose synthase (MhMTS) and a maltooligosyltrehalose trehalohydrolase (MhMTH), respectively. In this study, the two genes were...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Seo JS,An JH,Cheong JJ,Choi YD,Kim CH

    更新日期:2008-09-01 00:00:00

  • Interaction between the quorum sensing and stringent response regulation systems in the enterohemorrhagic Escherichia coli O157:H7 EDL933 strain.

    abstract::Quorum sensing and the stringent response are well-known regulation systems for the expression of virulence genes in enterohemorrhagic Escherichia coli (EHEC). However, how these two systems interact is not well known. E. coli strains with mutations in two regulation systems, ΔluxS (ECM101) and ΔluxSΔrelAΔspoT (ECM201...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Oh KH,Cho SH

    更新日期:2014-03-28 00:00:00

  • Exploring the effects of carbon sources on the metabolic capacity for shikimic acid production in Escherichia coli using in silico metabolic predictions.

    abstract::Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-s...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Ahn JO,Lee HW,Saha R,Park MS,Jung JK,Lee DY

    更新日期:2008-11-01 00:00:00

  • Inhibition of Herpes Simplex Viruses, Types 1 and 2, by Ginsenoside 20(S)-Rg3.

    abstract::Infections by herpes simplex viruses have an immense impact on humans, ranging from selflimiting, benign illness to serious, life-threatening diseases. While nucleoside analog drugs are available, resistance has been increasing and currently no vaccine exists. Ginsenosides derived from Panax ginseng have been document...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1908.08047

    authors: Wright S,Altman E

    更新日期:2020-01-28 00:00:00

  • Recombinant S-layer proteins of Lactobacillus brevis mediating antibody adhesion to calf intestine alleviated neonatal diarrhea syndrome.

    abstract::A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced i...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.0805.325

    authors: Khang YH,Park HY,Jeong YS,Kim JA,Kim YH

    更新日期:2009-05-01 00:00:00

  • Comparison of the Virulence-Associated Phenotypes of Five Species of Acinetobacter baumannii Complex.

    abstract::In this study, we compared the virulence-associated factors of Acinetobacter baumannii complex species. Sixty-three isolates of five A. baumannii complex species, including 19 A. baumannii, 15 A. nosocomialis, 13 A. seifertii, 13 A. pittii, and 3 A. calcoaceticus isolates, were included in this study. For all isolates...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1507.07076

    authors: Na IY,Chung ES,Jung CY,Kim DH,Shin J,Kang K,Kim ST,Ko KS

    更新日期:2016-01-01 00:00:00

  • Conessine Treatment Reduces Dexamethasone-Induced Muscle Atrophy by Regulating MuRF1 and Atrogin-1 Expression.

    abstract::Conessine, a steroidal alkaloid, is a potent histamine H3 antagonist with antimalarial activity. We recently reported that conessine treatment interferes with H₂O₂-induced cell death by regulating autophagy. However, the cellular signaling pathways involved in conessine treatment are not fully understood. Here, we rep...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1711.11009

    authors: Kim H,Jang M,Park R,Jo D,Choi I,Choe J,Oh WK,Park J

    更新日期:2018-04-28 00:00:00

  • Diversity and Plant Growth-Promoting Effects of Fungal Endophytes Isolated from Salt-Tolerant Plants.

    abstract::Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.2006.06050

    authors: Khalmuratova I,Choi DH,Woo JR,Jeong MJ,Oh Y,Kim YG,Lee IJ,Choo YS,Kim JG

    更新日期:2020-11-28 00:00:00

  • Effect of culture conditions and signal peptide on production of human recombinant N-acetylgalactosamine-6-sulfate sulfatase in Escherichia coli BL21.

    abstract::The production and characterization of an active recombinant N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in Escherichia coli BL21(DE3) has been previously reported. In this study, the effect of the signal peptide (SP), inducer concentration, process scale, and operational mode (batch and semi-continuous) on GALN...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1211.11044

    authors: Hernández A,Velásquez O,Leonardi F,Soto C,Rodríguez A,Lizaraso L,Mosquera Á,Bohórquez J,Coronado A,Espejo Á,Sierra R,Sánchez OF,Alméciga-Díaz CJ,Barrera LA

    更新日期:2013-05-01 00:00:00

  • WITHDRAWN: Antioxidant properties and antimicrobial activities of Bacillus mojavensis I4 lipopeptides and application in the biocontrol of potato dry rots caused by Fusarium solani.

    abstract::Ahead of Print article withdrawn by publisher. ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1811.11034

    authors: Imen G,Safa C,Mariam C,Radhia GB,Semia EC,Anissa H

    更新日期:2018-12-24 00:00:00

  • Alternative Carcinogenicity Screening Assay Using Colon Cancer Stem Cells: A Quantitative PCR (qPCR)-Based Prediction System for Colon Carcinogenesis.

    abstract::The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are ti...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1712.12043

    authors: Bak Y,Jang HJ,Shin JW,Kim SJ,Chun HW,Seo JH,No SH,Chae JI,Son DH,Lee SY,Hong J,Yoon DY

    更新日期:2018-04-28 00:00:00

  • Development of predictive mathematical model for the growth kinetics of Staphylococcus aureus by response surface model.

    abstract::A response surface model was developed for predicting the growth rates of Staphylococcus aureus in tryptic soy broth (TSB) medium as a function of combined effects of temperature, pH, and NaCl. The TSB containing six different concentrations of NaCl (0, 2, 4, 6, 8, and 10%) was adjusted to an initial of six different ...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Seo KY,Heo SK,Lee C,Chung DH,Kim MG,Lee KH,Kim KS,Bahk GJ,Bae DH,Kim KY,Kims CH,Ha SD

    更新日期:2007-09-01 00:00:00

  • Enhancement of L-lactic acid production in Lactobacillus casei from Jerusalem artichoke tubers by kinetic optimization and citrate metabolism.

    abstract::Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:

    authors: Ge XY,Qian H,Zhang WG

    更新日期:2010-01-01 00:00:00

  • Effect of Fc Fusion on Folding and Immunogenicity of Middle East Respiratory Syndrome Coronavirus Spike Protein.

    abstract::Middle East respiratory syndrome coronavirus (MERS-CoV) induces severe respiratory impairment with a reported mortality rate of ~36% in humans. The absence of clinically available MERS-CoV vaccines and treatments to date has resulted in uncontrolled incidence and propagation of the virus. In vaccine design, fusion wit...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1903.03043

    authors: Chun J,Cho Y,Park KH,Choi H,Cho H,Lee HJ,Jang H,Kim KH,Oh YK,Kim YB

    更新日期:2019-05-28 00:00:00

  • Effect of Corynebacterium glutamicum on Livestock Material Burial Treatment.

    abstract::In recent years, foot-and-mouth disease has occurred in all parts of the world. The animals with the disease are buried in the ground; therefore, their concentration could affect ground or groundwater. Moreover, the complete degradation of carcasses is not a certainty, and their disposal is important to prevent humans...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1603.03056

    authors: Kim BN,Cho HS,Cha Y,Park JK,Kim G,Kim YH,Min J

    更新日期:2016-08-28 00:00:00

  • Overexpression of YbeD in Escherichia coli Enhances Thermotolerance.

    abstract::Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. c...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1901.01036

    authors: Kim S,Kim Y,Yoon SH

    更新日期:2019-03-28 00:00:00

  • Characterization of Microbial Communities in Chinese Rice Wine Collected at Yichang City and Suzhou City in China.

    abstract::Two typical microbial communities from Chinese rice wine fermentation collected in Yichang city and Suzhou city in China were investigated. Both communities could ferment glutinous rice to rice wine in 2 days. The sugar and ethanol contents were 198.67 and 14.47 mg/g, respectively, for rice wine from Yichang city, and...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1704.04029

    authors: Lü Y,Gong Y,Li Y,Pan Z,Yao Y,Li N,Guo J,Gong D,Tian Y,Peng C

    更新日期:2017-08-28 00:00:00

  • Rice straw-decomposing fungi and their cellulolytic and xylanolytic enzymes.

    abstract::Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cell...

    journal_title:Journal of microbiology and biotechnology

    pub_type: 杂志文章

    doi:10.4014/jmb.1107.07022

    authors: Lee S,Jang Y,Lee YM,Lee J,Lee H,Kim GH,Kim JJ

    更新日期:2011-12-01 00:00:00