Simultaneous detection and identification of Bacillus cereus group bacteria using multiplex PCR.

abstract：:We developed a novel immunochromatographic assay (ICA) (EZ-Step VanA rapid kit; Dinona, Korea) for the detection of VanA ligase from vancomycin-resistant enterococci (VRE). Of eight monoclonal antibodies screened by ELISAs, the VanA ligase ICA constructed with 1H9 plus 3G11 showed the greatest reactivity. The detectio...

journal_title：Journal of microbiology and biotechnology

authors： Ji GY,Song HG,Son BR,Hong SB,Kim JW,Shin KS

更新日期：2014-03-28 00:00:00

abstract：:Several isolates of Bacillus thuringiensis (Bt) were screened for the vegetative insecticidal protein (Vip) effective against sap-sucking insect pests. Screening results were based on LC(50) values against cotton aphid (Aphis gossypii), one of the dangerous pests of various crop plants including cotton. Among the isol...

journal_title：Journal of microbiology and biotechnology

authors： Sattar S,Maiti MK

更新日期：2011-09-01 00:00:00

abstract：:Screening of a gene library from Paenibacillus sp. PBS-2 generated in Escherichia coli led to the identification of a clone with lipolytic activity. Sequence analysis showed an open reading frame encoding a polypeptide of 378 amino acid residues with a predicted molecular mass of 42 kDa. The esterase displayed 69% and...

journal_title：Journal of microbiology and biotechnology

authors： Kim YO,Park IS,Nam BH,Kim DG,Jee YJ,Lee SJ,An CM

更新日期：2014-09-01 00:00:00

abstract：:Beta-1,3-glucans enhance immune reactions such as antitumor, antibacterial, antiviral, anticoagulatory, and wound healing activities. beta-1,3-Glucans have various functions depending on the molecular weight, degree of branching, conformation, water solubility, and intermolecular association. The molecular weight of t...

journal_title：Journal of microbiology and biotechnology

authors： Shim JH,Sung KJ,Cho MC,Choi WA,Yang Y,Lim JS,Yoon DY

更新日期：2007-09-01 00:00:00

abstract：:Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The ...

journal_title：Journal of microbiology and biotechnology

authors： Hong I,Kim YS,Choi SG

更新日期：2010-02-01 00:00:00

abstract：:The identification of bacterial pathogens to humans is critical for environmental microbial risk assessment. However, current methods for identifying pathogens in environmental samples are limited in their ability to detect highly diverse bacterial communities and accurately differentiate pathogens from commensal bact...

journal_title：Journal of microbiology and biotechnology

authors： Yang J,Howe A,Lee J,Yoo K,Park J

更新日期：2020-09-28 00:00:00

abstract：:Polyester cloth (PC) was selected as a prospective inexpensive substitute separator material for microbial fuel cells (MFCs). PC was compared with a traditional Nafion proton exchange membrane (PEM) as an MFC separator by analyzing its physical and electrochemical properties. A single layer of PC showed higher mass tr...

journal_title：Journal of microbiology and biotechnology

authors： Kim T,Kang S,Sung JH,Kang YK,Kim YH,Jang JK

更新日期：2016-12-28 00:00:00

abstract：:The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protectio...

journal_title：Journal of microbiology and biotechnology

authors： Lee HS,Kim Y

更新日期：2020-07-28 00:00:00

abstract：:Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinas...

journal_title：Journal of microbiology and biotechnology

authors： Jang MK,Lee DG,Kim NY,Yu KH,Jang HJ,Lee SW,Jang HJ,Lee YJ,Lee SH

更新日期：2009-10-01 00:00:00

abstract：:A cDNA encoding cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum and ChainA, stefin B te...

journal_title：Journal of microbiology and biotechnology

authors： Li S,Guo D,Zhao B,Ye J,Tian J,Ren W,Ju Y,Cui P,Li R

更新日期：2010-08-01 00:00:00

abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Qian H,Zhang WG

更新日期：2010-01-01 00:00:00

abstract：:A bacterium, Pseudomonas aeruginosa BM114, capable of accumulating a blend of medium-chain-length (MCL)- and short-chain-length (SCL)-polyhydroxyalkanoic acid (PHA), was isolated. Salicylic acid (SA), without being metabolized, was found to specifically inhibit only the accumulation of MCL-PHA without affecting cell g...

journal_title：Journal of microbiology and biotechnology

authors： Rho JK,Choi MH,Shim JH,Lee SY,Woo MJ,Ko BS,Chi KW,Yoon SC

更新日期：2007-12-01 00:00:00

abstract：:Soils amended for long-term with high levels of compost demonstrated greater abundance of bacterial members of the phylum Bacteroidetes whereas a decreasing trend in the relative abundance of phylum Acidobacteria was noted with increasing levels of compost. Metabolic profiles predicted by PICRUSt demonstrated differen...

journal_title：Journal of microbiology and biotechnology

authors： Samaddar S,Han GH,Chauhan P,Chatterjee P,Jeon S,Sa T

更新日期：2019-02-28 00:00:00

abstract：:Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange c...

journal_title：Journal of microbiology and biotechnology

authors： Heo S,Lee SK,Lee CH,Min SG,Park JS,Kim HY

更新日期：2007-04-01 00:00:00

abstract：:To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: lahA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of ...

journal_title：Journal of microbiology and biotechnology

authors： Oh IJ,Lee HW,Park CH,Lee SY,Lee J

更新日期：2008-05-01 00:00:00

abstract：:Platelet-activating factor receptor (PAFR) plays an important role in bacterial infection and inflammation. We examined the effect of the bacterial cell wall components lipopolysaccharide (LPS) and lipoteichoic acid (LTA) from Lactobacillus plantarum (pLTA) and Staphylococcus aureus (aLTA) on PAFR expression in THP-1,...

journal_title：Journal of microbiology and biotechnology

authors： Kim H,Jung BJ,Jeong J,Chun H,Chung DK

更新日期：2014-08-01 00:00:00

abstract：:Gene delivery that provides targeted delivery of therapeutic genes to the cells of a lesion enhances therapeutic efficacy and reduces toxic side effects. This process is especially important in cancer therapy when it is advantageous to avoid unwanted damage to healthy, normal cells. Incorporating cancer-specific ligan...

journal_title：Journal of microbiology and biotechnology

authors： Tu Y,Kim JS

更新日期：2010-04-01 00:00:00

abstract：:Phenol-soluble modulins (PSMs) are responsible for regulating biofilm formation, persister cell formation, pmtR expression, host cell lysis, and anti-bacterial effects. To determine the effect of psm deletion on methicillin-resistant Staphylococcus aureus, we investigated psm deletion mutants including Δpsmα, Δpsmβ, a...

journal_title：Journal of microbiology and biotechnology

authors： Song HS,Bhatia SK,Choi TR,Gurav R,Kim HJ,Lee SM,Park SL,Lee HS,Joo HS,Kim W,Seo SO,Yang YH

更新日期：2021-01-28 00:00:00

abstract：:In this study, three of the representative EDCs, 17beta-estradiol, bisphenol A, and styrene, were employed to find their mode of toxic actions in E. coli. To accomplish this, four different stress response genes, recA, katG, fabA, and grpE genes, were used as a representative for DNA, oxidative, membrane, or protein d...

journal_title：Journal of microbiology and biotechnology

authors： Kim YS,Min J,Hongz HN,Park JH,Park KS,Gu MB

更新日期：2007-08-01 00:00:00

abstract：:Leaves are the major biomass-producing organs in herbaceous plants and mainly develop during vegetative stage by activities of shoot apical meristem. There is a strong correlation between leaf number and bolting, a characteristic phenotype during the transition to reproductive phase in Arabidopsis thaliana. In order t...

journal_title：Journal of microbiology and biotechnology

authors： Lee BH

更新日期：2009-06-01 00:00:00

abstract：:This study was done to establish a mouse model for catheter-related biofilm infection suitable to bioluminescence imaging (BLI). Biofilm formation of Pseudomonas aeruginosa (P. aeruginosa) Xen5 grown on catheter disks in vitro and in an implanted mouse model was real-time monitored during a 7-day study period using BL...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Yin H,Xu X,Cheng Y,Cai Y,Wang R

更新日期：2015-10-01 00:00:00

abstract：:L-Arabinose, a five carbon sugar, has not been considered as an important bioresource because most studies have focused on D-xylose, another type of five-carbon sugar that is prevalent as a monomeric structure of hemicellulose. In fact, L-arabinose is also an important monomer of hemicellulose, but its content is much...

journal_title：Journal of microbiology and biotechnology

authors： Ye S,Kim JW,Kim SR

更新日期：2019-03-28 00:00:00

abstract：:A tightly regulated gene expression system composed of a single-copy target gene under the control of a lac promoter derivative and lacI gene in a multicopy plasmid is proposed, and its ability to control the flux of a metabolic pathway is demonstrated. A model system to control the flux of acetyl-CoA to acetyl phosph...

journal_title：Journal of microbiology and biotechnology

authors： Lee SG,Liao JC

更新日期：2008-02-01 00:00:00

abstract：:Tannase (Tan410) from a soil metagenomic library was immobilized on different supports, including mesoporous silica SBA-15, chitosan, calcium alginate, and amberlite IRC 50. Entrapment in calcium alginate beads was comparatively found to be the best method and was further characterized. The optimum pH of the immobiliz...

journal_title：Journal of microbiology and biotechnology

authors： Yao J,Chen Q,Zhong G,Cao W,Yu A,Liu Y

更新日期：2014-01-01 00:00:00

abstract：:In this study, we compared the virulence-associated factors of Acinetobacter baumannii complex species. Sixty-three isolates of five A. baumannii complex species, including 19 A. baumannii, 15 A. nosocomialis, 13 A. seifertii, 13 A. pittii, and 3 A. calcoaceticus isolates, were included in this study. For all isolates...

journal_title：Journal of microbiology and biotechnology

authors： Na IY,Chung ES,Jung CY,Kim DH,Shin J,Kang K,Kim ST,Ko KS

更新日期：2016-01-01 00:00:00

abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...

journal_title：Journal of microbiology and biotechnology

authors： Lim HD,Cheong DE,Shin HJ,Kim GJ

更新日期：2010-11-01 00:00:00

abstract：:Our previous report determined that miR-144 is a key regulator of intestinal epithelial permeability in irritable bowel syndrome with diarrhea (IBS-D) rats. Recent evidence has shown that lactobacilli play an important role in the relief of IBS-D symptoms. However, few studies have addressed the mechanisms by which mi...

journal_title：Journal of microbiology and biotechnology

authors： Hou Q,Huang Y,Wang Y,Liao L,Zhu Z,Zhang W,Liu Y,Li P,Chen X,Liu F

更新日期：2020-10-28 00:00:00

abstract：:Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fe...

journal_title：Journal of microbiology and biotechnology

authors： Kang D,Li Z,Ji GE

更新日期：2018-05-28 00:00:00

abstract：:The diversity elucidation by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of Gram-positive over Gram-negative bacteria within the Kangra valley in Himachal P...

journal_title：Journal of microbiology and biotechnology

authors： Gulati A,Sood S,Rahi P,Thakur R,Chauhan S,Chawla I

更新日期：2011-06-01 00:00:00

abstract：:Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...

journal_title：Journal of microbiology and biotechnology

authors： Yang SJ,Ko JA,Kim HS,Jo MH,Lee HN,Park BR,Kim Y

更新日期：2018-12-28 00:00:00