Abstract:
:To investigate clonal variations of recombinant Chinese hamster ovary (rCHO) clones in response to culture pH and temperature, serum-free suspension cultures of two antibody-producing CHO clones (clones A and B), which were isolated from the same parental clone by the limiting dilution method, were performed in a bioreactor at pH values in the range of 6.8-7.6, and two different temperatures, 33 degrees C and 37 degrees C. In regard to cell growth, clone A and clone B displayed similar responses to temperature, although their degree of response differed. In contrast, clones A and B displayed different responses to temperature in regard to antibody production. In the case of clone A, no significant increase in maximum antibody concentration was achieved by lowering the culture temperature. The maximum antibody concentration obtained at 33 degrees C (pH 7.4) and 37 degrees C (pH 7.0) were 82.0 +/- 2.6 and 73.2 +/- 4.1 microg/ml, respectively. On the other hand, in the case of clone B, an approximately 2.5-fold increase in maximum antibody concentration was achieved by lowering the culture temperature. The enhanced maximum antibody concentration of clone B at 33 degrees C (132.6 +/- 14.9 microg/ml at pH 7.2) was due to not only enhanced specific antibody productivity but also to prolonged culture longevity. At 33 degrees C, the culture longevity of clone A also improved, but not as much as that of clone B. Taken together, CHO clones derived from the same parental clone displayed quite different responses to culture temperature and pH with regards antibody production, suggesting that environmental parameters such as temperature and pH should be optimized for each CHO clone.
journal_name
J Microbiol Biotechnoljournal_title
Journal of microbiology and biotechnologyauthors
Kim HS,Lee GMsubject
Has Abstractpub_date
2007-05-01 00:00:00pages
712-20issue
5eissn
1017-7825issn
1738-8872journal_volume
17pub_type
杂志文章abstract::Retrovirus tropism can be restricted by host cell factors such as Fv1, TRIM5alpha, and Lv1 that inhibit infection by targeting the incoming viral capsid. The Fv1 gene inhibits murine leukemia virus infection in mice, but the precise mechanism of Fv1-mediated restriction is poorly understood. Our previous studies had d...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-04-01 00:00:00
abstract::Two alternative pathways for methionine biosynthesis are known in Corynebacterium glutamicum: one involving transsulfuration (mediated by metB and metC) and the other involving direct sulthydrylation (mediated by metY). In this study, MetB (cystathionine gamma-synthase) and MetY (O-acetylhomoserine sulfhydrylase) from...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::A beta-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain o...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2009-03-01 00:00:00
abstract::Bacteriophages have gained substantial attention as biocontrol and biorecognition agents, substituting antibodies. In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is hi...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1711.11017
更新日期:2017-12-28 00:00:00
abstract::Based on its α-amylase activity at pH 5.0 and optimal pH of the crude enzyme, a strain (named B-5) with acid α-amylase production was screened. The B-5 strain was identified as Bacillus amyloliquefaciens through morphological, physiological, and biochemical characteristics analysis, as well as 16S rDNA phylogenetic an...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1409.09094
更新日期:2015-06-01 00:00:00
abstract::Copper-containing compounds are introduced into the environment through agricultural chemicals, mining, and metal industries and cause severe detrimental effects on ecosystems. Certain microorganisms exposed to these stressors exhibit molecular mechanisms to maintain intracellular copper homeostasis and avoid toxicity...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1111.11042
更新日期:2012-06-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging phlebovirus of the Phenuiviridae family that has been circulating in East Asian countries, such as China, Japan, and South Korea. Despite the increasing infection rates and relatively high mortality rate, there is limited information available re...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2008.08048
更新日期:2020-12-31 00:00:00
abstract::A novel antifungal protein produced by the fungal strain Penicillium citrinum W1, which was isolated from a Southwest Indian Ocean sediment sample, was purified and characterized. The culture supernatant of P. citrinum W1 inhibited the mycelial growth of some plant pathogenic fungi. After saturation of P. citrinum W1 ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1405.05008
更新日期:2014-10-01 00:00:00
abstract::The identification of bacterial pathogens to humans is critical for environmental microbial risk assessment. However, current methods for identifying pathogens in environmental samples are limited in their ability to detect highly diverse bacterial communities and accurately differentiate pathogens from commensal bact...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.2005.05033
更新日期:2020-09-28 00:00:00
abstract::Organic solvent-tolerant (OST) enzymes are widely applied in various industries due to their activity and stability in organic solvents, higher substrate solubility, and increased stereo-selectivity. However, the criteria for identifying OST enzymes largely remain unresolved. In this study, we compared the amino acid ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1806.06032
更新日期:2018-09-28 00:00:00
abstract::This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory con...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::Anthrax is a bacterial disease caused by the aerobic sporeforming bacterium Bacillus anthracis, which is an important pathogen owing to its ability to be used as a terror agent. B. anthracis spores can escape phagocytosis and initiate the germination process even in antimicrobial conditions, such as oxidative stress. ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1209.09070
更新日期:2013-06-28 00:00:00
abstract::TolC is an outer membrane porin protein and an essential component of drug efflux and type-I secretion systems in Gram-negative bacteria. TolC comprises a periplasmic alpha- helical barrel domain and a membrane-embedded beta-barrel domain. TdeA, a functional and structural homolog of TolC, is required for toxin and dr...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00
abstract::Controlling the residual glucose concentration is important for improving productivity in L-threonine fermentation. In this study, we developed a procedure to automatically control the feeding quantity of glucose solution as a function of ammonia-water consumption rate. The feeding ratio (RC/N) of glucose and ammonia ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1708.08072
更新日期:2018-02-28 00:00:00
abstract::Cellulase and xylanase are main hydrolysis enzymes for the degradation of cellulosic and hemicellulosic biomass, respectively. In this study, our aim was to develop and test the efficacy of a rapid, high-throughput method to screen hydrolytic-enzyme-producing microbes. To accomplish this, we modified the 3,5-dinitrosa...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1405.05052
更新日期:2014-11-28 00:00:00
abstract::To investigate the potential applications of bacterial heme, aminolevulinic acid synthase (HemA) was expressed in a Corynebacterium glutamicum HA strain that had been adaptively evolved against oxidative stress. The red pigment from the constructed strain was extracted and it exhibited the typical heme absorbance at 4...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1611.11010
更新日期:2017-03-28 00:00:00
abstract::Gene delivery that provides targeted delivery of therapeutic genes to the cells of a lesion enhances therapeutic efficacy and reduces toxic side effects. This process is especially important in cancer therapy when it is advantageous to avoid unwanted damage to healthy, normal cells. Incorporating cancer-specific ligan...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2010-04-01 00:00:00
abstract::A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-06-01 00:00:00
abstract::The exopolysaccharide (EPS) produced by Bacillus amyloliquefaciens GSBa-1 was isolated and purified by ethanol precipitation, and DEAE-cellulose and Sepharose CL-6B chromatographies. The molecular mass of the purified EPS was determined to be 54 kDa. Monosaccharide analysis showed that the EPS was composed of predomin...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1801.01012
更新日期:2018-08-28 00:00:00
abstract::Previous studies have demonstrated that repeated restraint stress in rodents produces increases in depression and anxietylike behaviors and alters the expression of corticotrophinreleasing factor (CRF) in the hypothalamus. The current study focused on the impact of Bupleurum falcatum (BF) extract administration on rep...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1110.10077
更新日期:2012-03-01 00:00:00
abstract::The cohesin complex holds sister chromatids together and prevents premature chromosome segregation until the onset of anaphase. Mcd1 (also known as Scc1), the α-kleisin subunit of cohesin, is a key regulatory subunit of the mitotic cohesin complex and is required for maintaining sister chromatid cohesion, chromosome o...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1501.01081
更新日期:2015-05-01 00:00:00
abstract::VP7, an outer capsid protein of grass carp reovirus (GCRV), was expressed and displayed on the surface of Saccharomyces cerevisiae for developing an efficient vaccine against hemorrhagic disease of grass carp. The result of flow cytometry analysis indicated that protein VP7 could be displayed on the surface of yeast c...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1505.05041
更新日期:2015-12-28 00:00:00
abstract::Four series (S, M, R, and W) of Alternaria longipes isolates were obtained based on consecutive induction with Dimethachlon (Dim) and ultraviolet irradiation. These isolates were then characterized according to their tolerance to Dim, sensitivity to osmotic stress, and phenotypic properties. All the induced Dim-resist...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The hepatitis C virus (HCV) RNA genome is replicated by an RNA replicase complex (RC) consisting of cellular proteins and viral nonstructural (NS) proteins, including NS5B, an RNAdependent RNA polymerase (RdRp) and key enzyme for viral RNA genome replication. The HCV RC is known to be associated with an intracellular ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1409.09063
更新日期:2014-12-28 00:00:00
abstract::Monoamine oxidase (MAO) is found in most cell types and catalyzes the oxidation of monoamines. Three anithiactins (A-C, modified 2-phenylthiazoles) isolated from Streptomyces sp. were tested for inhibitory activity of two isoforms, MAO-A and MAO-B. Anithiactin A was effective and selective for the inhibition of MAO-A,...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1505.05020
更新日期:2015-09-01 00:00:00
abstract::A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced i...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.0805.325
更新日期:2009-05-01 00:00:00
abstract::Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange c...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:
更新日期:2007-04-01 00:00:00
abstract::Cycloisomaltooligosaccharide glucanotransferase (CITase) was isolated from alkaliphilic Paenibacillus daejeonensis via an amino acid homology search for the reported CITase. The recombinant alkaliphilic CITase (PDCITase) from P. daejeonensis was expressed in an Escherichia coli expression system and purified as a sing...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1810.10007
更新日期:2018-12-28 00:00:00
abstract::A gene encoding lipolytic enzyme, lip7-3, was isolated from Bacillus sp. W130-35 isolated from a tidal mud flat. The gene encoded a protein of 215 amino acids with a signal peptide composed of 34 amino acid residues. Lip7-3 belonged to the family I.4 lipase and showed its maximal activity at pH 9.0 and 60°C. Its activ...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1507.07104
更新日期:2015-12-28 00:00:00
abstract::Ralstonia solanacearum causes bacterial wilt in a wide variety of host plant species and produces a melanin-like blackish-brown pigment in stationary phase when grown in minimal medium supplemented with tyrosine. To study melanin production regulation in R. solanacearum, five mutants exhibiting overproduction of melan...
journal_title:Journal of microbiology and biotechnology
pub_type: 杂志文章
doi:10.4014/jmb.1705.05049
更新日期:2017-09-28 00:00:00