Simultaneous assessment of autophagy and apoptosis using multispectral imaging cytometry.

abstract：:The yeast serine threonine kinase Atg1 appears to be a key regulator of autophagy and its kinase activity is crucial for autophagy induction. Recent reports have indicated that a mammalian Atg1 homolog, UNC-51-like kinase (ULK) 1, is required for autophagy. We found that ULK1 localizes to the autophagic isolation memb...

journal_title：Autophagy

authors： Hara T,Mizushima N

更新日期：2009-01-01 00:00:00

abstract：:Heme is an essential molecule expressed in many tissues where it plays key roles as the prosthetic group of several proteins involved in vital physiological and metabolic processes such as gas and electron transport. Structurally, heme is a tetrapyrrole ring containing an atom of iron (Fe) in its center. When released...

journal_title：Autophagy

authors： Travassos LH,Vasconcellos LR,Bozza MT,Carneiro LA

更新日期：2017-03-04 00:00:00

abstract：:The highly invasive and chemoresistant phenotype of pancreatic cancer highlights the urgency to identify prognostic biomarkers and novel therapeutic targets. Recently, we observed a significant correlation between shorter survival and loss of the cytoband 18q22.3. Here we investigated genes encoded by this cytoband, a...

journal_title：Autophagy

authors： Giovannetti E,Giaccone G

更新日期：2014-04-01 00:00:00

abstract：:Autophagy as a natural part of cellular homeostasis usually takes place unnoticed by neighboring cells. However, its co-occurrence with cell death may contribute to the clearance of these dying cells by recruited phagocytes. Autophagy associated with programmed cell death has recently been reported to be essential for...

journal_title：Autophagy

authors： Petrovski G,Zahuczky G,Májai G,Fésüs L

更新日期：2007-09-01 00:00:00

abstract：:The N-terminal amino acid of a protein is an essential determinant of ubiquitination and subsequent proteasomal degradation in the N-end rule pathway. Using para-chloroamphetamine (PCA), a specific inhibitor of the arginylation branch of the pathway (Arg/N-end rule pathway), we identified that blocking the Arg/N-end r...

journal_title：Autophagy

authors： Jiang Y,Lee J,Lee JH,Lee JW,Kim JH,Choi WH,Yoo YD,Cha-Molstad H,Kim BY,Kwon YT,Noh SA,Kim KP,Lee MJ

更新日期：2016-11-01 00:00:00

abstract：:Loss of the lysosomal chloride transport protein ClC-7 leads to complex phenotypes in mice and man, including osteopetrosis, accumulation of lysosomal storage material, and neurodegeneration. Using novel tissue-specific ClC-7 knockout mice, we have shown that upon loss of ClC-7, lysosomal degradation of endocytosed pr...

journal_title：Autophagy

authors： Wartosch L,Stauber T

更新日期：2010-01-01 00:00:00

abstract：:Dysfunction of macroautophagy/autophagy has been postulated as a major cellular toxicological response to nanomaterials. It has been reported that excessive autophagy activation, induced by silica nanoparticles (SiNPs), contributes to autophagy dysfunction, whereas little is known how SiNPs trigger autophagy activatio...

journal_title：Autophagy

authors： Ruan C,Wang C,Gong X,Zhang Y,Deng W,Zhou J,Huang D,Wang Z,Zhang Q,Guo A,Lu J,Gao J,Peng D,Xue Y

更新日期：2020-05-23 00:00:00

abstract：:Autophagy genes were first identified in the yeast system and some of their mammalian orthologues have also been characterized. Increasing lines of evidence indicate that various intracellular proteins, including G proteins, mammalian target of rapamycin (mTor) and Pl3K/Akt/PKB, of transmembrane signaling pathways are...

journal_title：Autophagy

authors： Jia G,Cheng G,Agrawal DK

更新日期：2007-01-01 00:00:00

abstract：:Atg8 and its homologs are essential for autophagosome formation in various species. In animal cells, Atg8 homologs have an additional function in clearance of damaged organelles and bacteria, acting as a landmark for selective autophagy. We have recently shown that OATL1, a Rab-GTPase-activating protein (Rab-GAP), is ...

journal_title：Autophagy

authors： Itoh T,Fukuda M

更新日期：2011-09-01 00:00:00

abstract：:Macroautophagy sequesters superflous cytosol and organelles into double-membraned autophagosomes. Over 30 autophagy-related (ATG) genes have been identified without elucidating the molecular details of autophagosome biogenesis. All proposed models for autophagosome formation require membrane fusion events (Fig. 1). Pr...

journal_title：Autophagy

authors： Krick R,Bremer S,Welter E,Eskelinen EL,Thumm M

更新日期：2011-02-01 00:00:00

abstract：:ULK1 and ATG13 assemble with RB1CC1/FIP200 and ATG101 to form a macroautophagy (hereafter autophagy) induction (ULK1) complex in higher eukaryotes. The yeast counterpart, the Atg1 complex, is comprised of Atg1 and Atg13 (ULK1 and ATG13 homologs), Atg17 (a proposed functional homolog of RB1CC1), and either the Atg101 s...

journal_title：Autophagy

authors： Popelka H,Klionsky DJ

更新日期：2015-11-02 00:00:00

abstract：:Macroautophagy is an intracellular catabolic process involved in the formation of multiple membrane structures ranging from phagophores to autophagosomes and autolysosomes. Dysfunction of macroautophagy is implicated in both physiological and pathological conditions. To date, 38 autophagy-related (ATG) genes have been...

journal_title：Autophagy

authors： Xie Y,Kang R,Sun X,Zhong M,Huang J,Klionsky DJ,Tang D

更新日期：2015-01-01 00:00:00

abstract：:microRNAs (miRNAs) are a class of small regulatory RNAs that regulate gene expression at the post-transcriptional level. miRNAs play important roles in the regulation of development, growth, and metastasis of cancer, and in determining the response of tumor cells to anticancer therapy. In recent years, they have also ...

journal_title：Autophagy

authors： Yu Y,Cao L,Yang L,Kang R,Lotze M,Tang D

更新日期：2012-05-01 00:00:00

abstract：:Autophagosomes arise in yeast and animals from the sealing of a cup-shaped double-membrane precursor, the phagophore. The concerted action of about 30 evolutionarily conserved autophagy related (ATG) proteins lies at the core of this process. However, the mechanisms allowing phagophore generation and its differentiati...

journal_title：Autophagy

authors： Le Bars R,Marion J,Satiat-Jeunemaitre B,Bianchi MW

更新日期：2014-10-01 00:00:00

abstract：:ISG15 (ISG15 ubiquitin-like modifier), a ubiquitin-like protein, is one of the major type I IFN (interferon) effector systems. ISG15 can be conjugated to target proteins (ISGylation) via the stepwise action of E1, E2, and E3 enzymes. Conjugated ISG15 can be removed (deISGylated) from target proteins by USP18 (ubiquiti...

journal_title：Autophagy

authors： Xu D,Zhang T,Xiao J,Zhu K,Wei R,Wu Z,Meng H,Li Y,Yuan J

更新日期：2015-04-03 00:00:00

abstract：:A major side effect of the powerful immunosuppressive drug cyclosporine (CsA) is the development of a chronic nephrotoxicity whose mechanisms are not fully understood. Recent data suggest that tubular cells play a central role in the pathogenesis of chronic nephropathies. We have shown that CsA is responsible for endo...

journal_title：Autophagy

authors： Pallet N,Bouvier N,Legendre C,Gilleron J,Codogno P,Beaune P,Thervet E,Anglicheau D

更新日期：2008-08-01 00:00:00

abstract：:ATM (ataxia telangiectasia mutated) protein is found associated with multiple organelles including synaptic vesicles, endosomes and lysosomes, often in cooperation with ATR (ataxia telangiectasia and Rad3 related). Mutation of the ATM gene results in ataxia-telangiectasia (A-T), an autosomal recessive disorder with de...

journal_title：Autophagy

authors： Cheng A,Tse KH,Chow HM,Gan Y,Song X,Ma F,Qian YXY,She W,Herrup K

更新日期：2020-08-14 00:00:00

abstract：:Neuroepithelial cells undergoing differentiation efficiently remodel their cytoskeleton and shape in an energy-consuming process. The capacity of autophagy to recycle cellular components and provide energy could fulfill these requirements, thus supporting differentiation. However, little is known regarding the role of...

journal_title：Autophagy

authors： Vázquez P,Arroba AI,Cecconi F,de la Rosa EJ,Boya P,de Pablo F

更新日期：2012-02-01 00:00:00

abstract：:In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update the...

journal_title：Autophagy

authors： Klionsky DJ,Abdalla FC,Abeliovich H,Abraham RT,Acevedo-Arozena A,Adeli K,Agholme L,Agnello M,Agostinis P,Aguirre-Ghiso JA,Ahn HJ,Ait-Mohamed O,Ait-Si-Ali S,Akematsu T,Akira S,Al-Younes HM,Al-Zeer MA,Albert ML,Albin RL

更新日期：2012-04-01 00:00:00

abstract：:Atg18p and Atg21p are two highly homologous yeast autophagy proteins. Atg18p functions in both autophagy and the selective Cvt-pathway, while the function of Atg21p is restricted to the Cvt-pathway. The yeast genome encodes with Ygr223cp (Hsv2p), a third member of this protein family. So far no function has been assig...

journal_title：Autophagy

authors： Krick R,Henke S,Tolstrup J,Thumm M

更新日期：2008-10-01 00:00:00

abstract：:Oxidative stress-induced granulosa cell (GCs) death represents a common reason for follicular atresia. Follicle-stimulating hormone (FSH) has been shown to prevent GCs from oxidative injury, although the underlying mechanism remains to be elucidated. Here we first report that the suppression of autophagic cell death v...

journal_title：Autophagy

authors： Shen M,Jiang Y,Guan Z,Cao Y,Li L,Liu H,Sun SC

更新日期：2017-08-03 00:00:00

abstract：:Earlier studies have shown that macroautophagy is not a constitutively activated process, however, the mechanism of activation is not fully understood. Here, we report that autophagy is a dynamic process in cancer cells in response to glucose starvation. In addition, we determined that FOXO1 turnover is involved in th...

journal_title：Autophagy

authors： Zhao Y,Li X,Ma K,Yang J,Zhou J,Fu W,Wei F,Wang L,Zhu WG

更新日期：2013-05-01 00:00:00

abstract：:Autophagy is a degradation pathway for the turnover of dysfunctional organelles or aggregated proteins in cells. Extracellular accumulation of beta-amyloid peptide has been reported to be a major cause of Alzheimer disease (AD) and large numbers of autophagic vacuoles accumulate in the brain of AD patient. However, ho...

journal_title：Autophagy

authors： Hung SY,Huang WP,Liou HC,Fu WM

更新日期：2009-05-01 00:00:00

abstract：:Macroautophagy ("autophagy") is regulated by the same insulin-amino acid-mTOR signaling pathway that controls protein synthesis. Although the literature does not so far include any direct studies confirming this, we expect autophagy to increase during insulin resistance. We discuss the possibility that this may be a u...

journal_title：Autophagy

authors： Meijer AJ,Codogno P

更新日期：2007-09-01 00:00:00

abstract：:In a series of studies into the fate of endothelial cells exposed to non-enzymatically glycated collagen I, a model of cytotoxic molecules relevant to diabetic vasculopathy, we demonstrate that cells either undergo apoptosis or become prematurely senescent despite relatively spared telomeres and telomerase activity. O...

journal_title：Autophagy

authors： Patschan S,Goligorsky MS

更新日期：2008-05-01 00:00:00

abstract：:Understanding the role of autophagy in cancer has been limited by the inability to measure this dynamic process in formalin-fixed tissue. We considered that 3-dimensional models including ex vivo tumor, such as we have developed for studying mesothelioma, would provide valuable insights. Using these models, in which w...

journal_title：Autophagy

authors： Follo C,Barbone D,Richards WG,Bueno R,Broaddus VC

更新日期：2016-07-02 00:00:00

abstract：:A hallmark of macroautophagy is the formation of autophagosomes, double-membrane vesicles that enwrap cellular components destined for lysosomal degradation. We examined autophagosomal protein dynamics under various inducing stimuli using a comprehensive mass spectrometry-based proteomics approach in combination with ...

journal_title：Autophagy

authors： Becker AC,Bunkenborg J,Eisenberg T,Harder LM,Schroeder S,Madeo F,Andersen JS,Dengjel J

更新日期：2012-06-01 00:00:00

abstract：:Autophagy is a highly conserved degradation pathway for intracellular macromolecules and organelles. Among those characterized autophagy regulators, the ubiquitin-like protein Atg8 is found to be a membrane modifier that both regulates biogenesis of transport vesicles and interacts with the cargo receptor Atg19 for se...

journal_title：Autophagy

authors： Ho KH,Chang HE,Huang WP

更新日期：2009-05-01 00:00:00

abstract：:In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy in fusion-induced nuclear degradation. A search of the F...

journal_title：Autophagy

authors： Corral-Ramos C,Roca MG,Di Pietro A,Roncero MI,Ruiz-Roldán C

更新日期：2015-01-01 00:00:00

abstract：:Phagocytosis plays a central role in immunity and tissue homeostasis. After internalization of cargo into single-membrane phagosomes, these compartments undergo a maturation sequences that terminates in lysosome fusion and cargo degradation. Components of the autophagy pathway have recently been linked to phagosome ma...

journal_title：Autophagy

authors： Cemma M,Grinstein S,Brumell JH

更新日期：2016-09-01 00:00:00