Abstract:
:The peptide hormone, GnRH has been hypothesized to play a direct antitumoral role in certain kinds of breast cancer. This direct effect is considered to function via high affinity membrane receptors to GnRH that have been demonstrated in tumors. However, no routine assay for GnRH receptors exist limiting further research in elucidating the mechanisms of GnRH action during treatment and both the clinical usefulness of this measure and the development or refinement of GnRH treatments. Part of the problem has been that previously reported procedures require expensive, difficult (for routine clinical laboratories) iodination of specific GnRH analogs. The use of iodinated substrates present the additional problems of radioactive waste disposal and safety of laboratory personal. Further, the use of different analogs complicates the comparison of data between laboratories. We present an assay that utilizes tritiated natural GnRH in order to assay the native receptor binding and reduce both safety problems and cost of assay/waste disposal. The use of nitrocellulose filters treated overnight with 1% BSA resolved the problems of high blank values. Kinetic data demonstrate that this assay is sensitive, accurate and give results comparable with other methods. We also show that the membranes derived from the tumor and the cell cultures may be frozen at -80 degrees C for up to 90 days without affecting the results of the assay.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Reshkin S,Pezzetta A,Albarani V,Schittulli F,Paradiso Asubject
Has Abstractpub_date
1995-08-01 00:00:00pages
371-5issue
2eissn
1019-6439issn
1791-2423journal_volume
7pub_type
杂志文章abstract::Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apopt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::The expression of proinflammatory cytokines in various malignant neoplasms is widely considered to represent the host immune response to tumor development. The role of interferon (IFN)γ in head and neck squamous cell carcinoma, and its association with endoplasmic reticulum (ER) stress pathways, remains a subject of o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4590
更新日期:2018-12-01 00:00:00
abstract::The murine double minute-2 (MDM2) gene encodes a 90 kDa protein which binds and inactivates the protein product of the p53 tumor suppressor gene. To elucidate the potential role of MDM2 in benign and malignant hyperproliferative conditions in the pancreas, we studied MDM2 expression in cultured human pancreatic cancer...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.6.1279
更新日期:1994-12-01 00:00:00
abstract::In a previous study, it was demonstrated that Rhein lysinate (RHL) inhibited HeLa cell proliferation via a specific mechanism. The aim of the present study was to clarify the mechanism of RHL by investigating its effect on mitochondrial damage and cell apoptosis. The results indicated that RHL inhibited cell growth an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4554
更新日期:2018-11-01 00:00:00
abstract::Cervical cancer constitutes the second most common cancer in women. It is evident from earlier studies that epidermal growth factor (EGF) is a mitogen, in that it mimics the function of estrogen by mediating cross-talk with other oncoproteins. Although epidermal growth factor recept...
journal_title:International journal of oncology
pub_type: 杂志文章,收录出版
doi:10.3892/ijo.2011.1211
更新日期:2012-01-01 00:00:00
abstract::Survivin is a member of the inhibitor of apoptosis family, which is known to inhibit mitochondrial apoptosis. Survivin is highly expressed in cancers and plays an important role in cancer cell survival, and increased survivin expression is an unfavorable prognostic marker in cancer patients. YM155, a novel small-molec...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3077
更新日期:2015-09-01 00:00:00
abstract::One of the early events of apoptosis is proteolysis. The enzyme(s) involved in this event appear to be the serine protease(s), inasmuch as the reversible or irreversible inhibitors of serine proteases prevent DNA degradation and abrogate other signs of apoptosis in several cell systems (Gorczyca et al, Int J Oncol 1: ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.4.627
更新日期:1993-10-01 00:00:00
abstract::Identification of aberrant expression patterns of genes in prostate cancer (PCa) is a key step towards the development of effective therapies. Prostate-specific antigen (PSA) levels are commonly measured for the early detection of PCa, but which itself is still not an ideal biomarker. We analysed the expression patter...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2117
更新日期:2013-12-01 00:00:00
abstract::Developing simple and effective approaches to detect tumor markers will be critical for early diagnosis or prognostic evaluation of prostate cancer treatment. Prostate‑specific membrane antigen (PSMA) has been validated as an important tumor marker for prostate cancer progression including angiogenesis and metastasis....
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2256
更新日期:2014-03-01 00:00:00
abstract::We have isolated a new -ene, named BAC, which is the human equivalent of the murine H19 gene and is highly expressed in most fetal tissues and in a variety of fresh tumors. BAC was analyzed in 130 untreated invasive carcinomas of different types. The frequency of BAC-expressing cancers as well as the level of expressi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.5.753
更新日期:1993-05-01 00:00:00
abstract::The Warburg effect is a unique metabolic feature of the majority of tumor cells and is closely related to chemotherapeutic resistance. Pyruvate dehydrogenase kinase 1 (PDK1) is considered a 'switch' that controls the fate of pyruvate in glucose metabolism. However, to date, to the best of our knowledge, there are only...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5098
更新日期:2020-09-01 00:00:00
abstract::Proteasome activator 28γ (PA28γ) binds to and activates the proteasome in an ATP-independent manner to promote mainly ubiquitin-independent protein degradation in cells. Previously, four transcript variants of PA28γ have been identified, which have been closely correlated with the progression of cancers. In the presen...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2980
更新日期:2015-07-01 00:00:00
abstract::Acquirement of multi-drug resistance by tumor cells represents a major obstacle in the management of prostate cancer. Such resistance was demonstrated in the androgen-independent DU-145 cells in response to paclitaxel and the mechanisms by which these cell develops resistance was not understood. The objective of this ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::To test the effects of combined transduction of a suicide gene and genes coding for various immunostimulatory factors on the oncogenicity and immunogenicity of TC-1 cells (HPV-16 transformed C57BL/6 mouse cells), several bicistronic recombinant adeno-associated viruses (rAAV) were constructed. Each of these constructs...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-03-01 00:00:00
abstract::The cytosine analogue 5'-azacytidine (5'-aza) induces DNA hypomethylation by inhibiting DNA methyltransferase. In clinical trials, 5'-aza is widely used in epigenetic anticancer treatments. Accumulated evidence shows that cyclooxygenase-2 (COX-2) is overexpressed in various cancers, indicating that it may play a criti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3110
更新日期:2015-10-01 00:00:00
abstract::Chemotherapy (CT) resistance in ovarian cancer is related to multiple factors, and assessment of these factors is necessary for the development of new drugs and therapeutic regimens. In an effort to identify such determinants, we evaluated the expression of approximately 21,000 genes using DNA microarray screening in ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Experimental evidence indicates that prostate apoptosis response-4 (Par-4, also known as PAWR) is a key regulator of cancer cell survival and may be a target for cancer-selective targeted therapeutics. Par-4 was first identified in prostate cancer cells undergoing apoptosis. Both intracellular and extracellular Par-4 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1983
更新日期:2013-08-01 00:00:00
abstract::The support mechanisms that are involved in lymph-node metastasis of oral squamous cell carcinoma (OSCC) remain largely unknown. Recent studies have demonstrated that tumor cells express chemokine receptors and use chemokines to metastasize to the target organ in many malignancies in humans. In this study, we examined...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::Reduced expression of a cyclin-dependent kinase inhibitor, p27, has been reported to be associated with poor prognosis in several human cancers. The aim of this study was to investigate the potential role of p27 in ovarian cancer development and progression. Immunohistochemical expression of p27 was determined using 1...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.5.953
更新日期:2001-05-01 00:00:00
abstract::The α6 integrin subunit (α6) has been implicated in cancer cell migration and in the progression of several malignancies, but its role in tumor angiogenesis is unclear. In mice, anti-α6 blocking antibodies reduce tumor angiogenesis, whereas Tie1-dependent α6 gene deletion enhances neovessel formation in melanoma and l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2631
更新日期:2014-11-01 00:00:00
abstract::Magnolol, a neolignan from the traditional medicinal plant Magnolia obovata, has been shown to possess neuroprotective, anti-inflammatory, anticancer and anti-angiogenic activities. However, the precise mechanism of the anti-angiogenic activity of magnolol remains to be elucidated. In the present study, the anti-angio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1959
更新日期:2013-08-01 00:00:00
abstract::The Drosophila ras2 and rop gene pair are governed by a common promoter. Characterisation of the Drosophila ras2/rop bidirectional promoter has revealed that a single major protein complex (M), composed of two subunits (factors A and B) interacts with two central promoter domains (regions A and B). To define the trans...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.5.1185
更新日期:1995-11-01 00:00:00
abstract::Neuroblastoma is the second most common extracranial malignant solid tumor that occurs in childhood, and metastasis is one of the major causes of death in neuroblastoma patients. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. T...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3954
更新日期:2017-05-01 00:00:00
abstract::Many genes that are frequently mutated in human cancer are known to be involved in the control of normal cellular proliferation. One of the genes involved in DNA mismatch repair is hMLH1, defective mutations of which are found in some familial and various sporadic cancers. Although the DNA mismatch repair activity of ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.3.609
更新日期:1998-03-01 00:00:00
abstract::Dose is a critical determinant of the efficacy of chemotherapy. The dose response curve for virtually all anticancer agents is steep in both in vitro and in vivo experimental studies. However the application of the steep dose-response curve in the clinic has been troublesome for many years because of dose limiting bon...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Extracellular matrix breakdown as well as increased expression in cancer cells and tumor microvascular endothelial cells make matrix metalloproteinase 2 (MMP2) an attractive target for cancer treatment. By taking advantage of MMP2's properties, an MMP2 cleavable melittin/avidin conjugate was designed. Melittin alone i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Autocrine/paracrine stimulation of KIT has been observed in colorectal carcinoma (CRC) cell lines. We investigated the expression of KIT and stem cell factor (SCF) in CRC in comparison with premalignant colon lesions and normal colonic mucosa to assess the prognostic and therapeutic relevance of this receptor/ligand s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-10-01 00:00:00
abstract::Cholangiocarcinoma (CCA) is a lethal malignancy which occurs with relatively high incidence in Thailand. This cancer is often difficult to diagnose and associated with high mortality. The secretome, containing the secreted proteins from cells, are potentially useful as biomarkers of cancers. Since three-dimensional (3...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2636
更新日期:2014-11-01 00:00:00
abstract::Neuroblastoma shows complex patterns of genetic aberrations including MYCN amplification, deletion of chromosome 1p or 11q, and gain of chromosome 17q. The 17q gain is frequently observed in high-risk neuroblastomas, however, the candidate genes still remain elusive. In the present study, we integrated the data of com...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000219
更新日期:2009-04-01 00:00:00
abstract::The Pleomorphic adenoma gene 1 (PLAG1) is involved in various human neoplasias, including pleomorphic adenomas of the salivary glands. Moreover, the oncogenic role of PLAG1 was clearly demonstrated in two independent PLAG1 transgenic mouse founders, in which PLAG1 expression could be targeted to different tissues usin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-05-01 00:00:00