Abstract:
:Developing simple and effective approaches to detect tumor markers will be critical for early diagnosis or prognostic evaluation of prostate cancer treatment. Prostate‑specific membrane antigen (PSMA) has been validated as an important tumor marker for prostate cancer progression including angiogenesis and metastasis. As a type II membrane protein, PSMA can be constitutively internalized from the cell surface into endosomes. Early endosomes can fuse with multivesicular bodies (MVB) to form and secrete exosomes (40-100 nm) into the extracellular environment. Herein, we tested whether some of the endosomal PSMA could be transferred to exosomes as an extracellular resource for PSMA. Using PSMA-positive LNCaP cells, the secreted exosomes were collected and isolated from the cultured media. The vesicular structures of exosomes were identified by electron microscopy, and exosomal marker protein CD9 and tumor susceptibility gene (TSG 101) were confirmed by western blot analysis. Our present data demonstrate that PSMA can be enriched in exosomes, exhibiting a higher content of glycosylation and partial proteolysis in comparison to cellular PSMA. An in vitro enzyme assay further confirmed that exosomal PSMA retains functional enzymatic activity. Therefore, our data may suggest a new role for PSMA in prostate cancer progression, and provide opportunities for developing non-invasive approaches for diagnosis or prognosis of prostate cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Liu T,Mendes DE,Berkman CEdoi
10.3892/ijo.2014.2256subject
Has Abstractpub_date
2014-03-01 00:00:00pages
918-22issue
3eissn
1019-6439issn
1791-2423journal_volume
44pub_type
杂志文章abstract::The over-expression of urokinase receptor in breast cancer cells results in increased tumor invasion, growth and metastasis. As LY-6K belongs to the Ly6/uPAR superfamily, we assessed the association of LY-6K with metastasis in breast cancer cell lines and how the LY-6K gene mediates cancer cell metastasis. The migrati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000371
更新日期:2009-09-01 00:00:00
abstract::Prostate cancer, the third most common cancer in men worldwide, varies substantially according to geographic region and race/ethnicity. Obesity and associated endocrine variation are foremost among the risk factors that may underlie these regional and ethnic differences. The association between obesity and prostate ca...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
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abstract::Sentinel node (SN) mapping and biopsy is a procedure that accurately stages the regional lymph node (LN) basin. Defined patterns of lymphatic drainage allow intraoperative determination of the first (sentinel) lymph node in the regional basin, and the absence of metastatic disease in the SN accurately reflects the abs...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
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abstract::Because of the constant inflow of information and educational efforts by many organizations, public attention is increasingly focused in finding information about strategies for prevention of cancer. Because of the research efforts of the last twenty years it is now increasingly appreciated that changes in dietary hab...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1387
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journal_title:International journal of oncology
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doi:10.3892/ijo.2015.3241
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.3.401
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pub_type: 杂志文章
doi:10.3892/ijo.15.5.1039
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2647
更新日期:2014-12-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.2.347
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
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journal_title:International journal of oncology
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.968
更新日期:2011-05-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:International journal of oncology
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doi:
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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