Abstract:
:Proteasome activator 28γ (PA28γ) binds to and activates the proteasome in an ATP-independent manner to promote mainly ubiquitin-independent protein degradation in cells. Previously, four transcript variants of PA28γ have been identified, which have been closely correlated with the progression of cancers. In the present study, we predicted the alternative splicing of PA28γ via the bioinformatics tool ASPicDB and 49 splices were predicted. Then, we cloned some new segment according to predication in oral cancer cells using reverse transcription PCR and a novel variant of PA28γ was found. The novel transcript encodes a truncated form compared with other isoforms of PA28γ. However, it contains most of the conserved residues and the 'activation loop' of the PA28γ family. In order to explore its function, we overexpressed the variant in HEK293 cells and demonstrated that this variant is likely to further regulate cell cycle and apoptosis via regulating p53 and the mouse double minute2 homolog (Mdm2).
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Xu X,Liu D,Ji N,Li T,Li L,Jiang L,Li J,Zhang P,Zeng X,Chen Qdoi
10.3892/ijo.2015.2980subject
Has Abstractpub_date
2015-07-01 00:00:00pages
188-94issue
1eissn
1019-6439issn
1791-2423journal_volume
47pub_type
杂志文章abstract::Optimal conditions have been defined to grow primary cultures of malignant melanoma suitable for in vitro pharmacological studies. A feasibility of primary cultures was observed in 60% of 62 clinical melanoma lymph node metastases. The neoplastic nature of the cells grown in culture, as assessed by the highly specific...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.6.619
更新日期:1992-11-01 00:00:00
abstract::We have previously reported that Insulin-like growth factor (IGF-I) is a cellular growth factor of human transformed T-lymphocytes. To evaluate the role of IGF-I for nontransformed T-cells we investigated synthesis of IGF-I and the influence of an IGF-I antiserum, UBK487, during proliferation process. IGF-I is synthes...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.6.1003
更新日期:1993-06-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is characterized by extensive angiogenesis that is mostly orchestrated by the hypoxia inducible factor HIF-1. Deregulation of HIF-1 is believed to contribute to cancer initiation and progression. However, instances have been described in which loss of HIF-1 leads to more aggressive tumors...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000554
更新日期:2010-04-01 00:00:00
abstract::Placenta-specific protein 1 (PLAC1) expression is co-opted in numerous human cancers. As a consequence of PLAC1 expression, tumor cells exhibit enhanced proliferation and invasiveness. This characteristic is associated with increased aggressiveness and worse patient outcomes. Recently, the presence of the tumor suppre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3931
更新日期:2017-05-01 00:00:00
abstract::We investigated the expression and promoter methylation of dbpA in human hepatocellular carcinoma (HCC) and examined their correlation with clinicopathological features. In 96 paired samples of HCC and adjacent non-tumorous liver, and 10 normal liver specimens, dbpA mRNA was quantif...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1282
更新日期:2012-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding, endogenous, single-stranded RNAs. MiRNAs have been implicated in different areas such as the immune response, neural development, DNA repair, apoptosis, oxidative stress response and cancer. However, while the majority of miRNAs are found int...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2012.1647
更新日期:2012-12-01 00:00:00
abstract::Mos is a germ cell-specific serine/threonine protein kinase that plays an important role during meiotic divisions of oocytes. Upon expression in somatic cells, Mos causes cell cycle perturbations leading to neoplastic transformation. Mos activates the MAP kinase pathway in both oocytes and transformed somatic cells. T...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.5.1199
更新日期:1998-05-01 00:00:00
abstract::Neuroblastoma, originated from neural crest cells, is the most common extracranial solid tumor in childhood. In the present study, we evaluated in vitro the oncolytic effect of live-attenuated poliovirus on human neuroblastoma cell lines, and in vivo its therapeutic efficacy in human neuroblastoma-bearing athymic mice...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::We have examined six patients with multiple low grade, low stage superficial multifocal bladder tumors with surrounding tissues for loss of heterozygosity (LOH) and microsatellite instability at chromosome 3, totaling 76 samples. The majority (4/5) of the patients had LOH at or close to the fragile histidine triad (FH...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.1.203
更新日期:2001-01-01 00:00:00
abstract::Von Hippel-Lindau (VHL) disease is a rare autosomal dominant syndrome that is the main cause of inherited clear-cell renal cell carcinoma (ccRCC), which generally occurs in the form of multiple recurrent synchronized tumors. Affected patients are carriers of a germline mutation in the VHL tumor suppressor gene. Somati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4490
更新日期:2018-10-01 00:00:00
abstract::The CXC chemokine receptor 7 (CXCR7) has been reported to be involved in cell growth, metastasis and apoptosis in certain cancers. However, the function and molecular mechanisms of CXCR7 in human colorectal cancer (CRC) are still undefined. In the present study, sixty-eight cases of CRC tissues and corresponding adjac...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2547
更新日期:2014-10-01 00:00:00
abstract::Growth factors may play a role in autocrine or paracrine growth control of tumour cells. We have now examined the expression pattern in vivo by in situ hybridization (ISH) on a series of 13 ductal adenocarcinomas of the pancreas using the non-radioactive digoxigenin system to generate specific antisense orientated rib...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.6.1219
更新日期:1994-06-01 00:00:00
abstract::MicroRNA‑301a (miRNA/miR‑301a) and nuclear factor (NF)‑κB signaling play important roles in tumor invasion, migration and progression. However, the role of miRNA‑301a‑3p in human gastric cancer (GC), and specifically in the activation of NF‑κB signaling, remains unclear. The aim of the present study was to investigate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5072
更新日期:2020-08-01 00:00:00
abstract::The aim of the present study was to evaluate the effects of programmed cell death 4 (PDCD4) on cell proliferation and apoptosis, and to elucidate the potential role of the Jun N-terminal kinase (JNK)/c-Jun pathway in human bladder cancer (BCa) cells. Mixed BCa cells were transfected with plasmids containing PDCD4 (PDC...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4303
更新日期:2018-05-01 00:00:00
abstract::In the study of 69 matched pairs of lung tumors and normal lung parenchyma, the median activities of cathepsin B (CB) and cystein proteinase inhibitors (CPI's) in tumors were found to be increased 4.4-fold and twofold, respectively. The levels of CB and CPI activities did not correlate with TNM stages nor with cell di...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.1.77
更新日期:1994-07-01 00:00:00
abstract::Inhibin and activin, members of transforming growth factor beta (TGF beta) superfamily, have been localized immunohistochemically in fetal bovine heart endothelial cells (FBHE), suggesting that these cells produce inhibin/activin proteins. In addition, the mRNAs coding for alpha-, and beta A-subunits of inhibin/activi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.4.759
更新日期:1995-10-01 00:00:00
abstract::Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apopt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::The p16INK4 gene, encoding a cyclin-dependent kinase inhibitor, is frequently methylated in colorectal cancer, and a side-specific methylation frequency was suggested. To clarify the frequency of real loss of tumor suppressor function dependent on anatomical localization, we investigated 43 primary colorectal carcinom...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::MicroRNA (miRNA)-126 (miR-126) was reported to be downregulated and to act as a tumor suppressor in cancers of the lung, cervix, bladder and prostate. However, the functions of miR-126 in gastric cancer appear to be diverse and are largely unknown. MiR-126 was reported to act as a tumor suppressor by targeting the Crk...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2516
更新日期:2014-09-01 00:00:00
abstract::Metal-containing compounds have been extensively studied for many years as potent proteasome inhibitors. The 20S proteasome, the main component of the ubiquitin proteasome pathway, is one of the excellent targets in anticancer drug development. We recently reported that several copper complexes were able to inhibit ca...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3542
更新日期:2016-08-01 00:00:00
abstract::Selectivity of the anticancer agent PB-100 for malignant cells, already demonstrated using cell growth and viability evaluation, is now confirmed by microscopic observations. PB-100 is easily detected inside cells by its yellow color under visible light and by its blue fluorescence; it may be measured in isolated nucl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.1.81
更新日期:1995-07-01 00:00:00
abstract::Enhanced Notch signaling contributes to uncontrolled cell growth and cell death resistance in cancer. Here, we demonstrate that in colorectal carcinoma cells the Notch1-dependent activation of cell cycle and proliferation is mediated by repression of the cyclin-dependent kinase inhibitor (CDKI) p27. The half-life of p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2140
更新日期:2013-12-01 00:00:00
abstract::Over the past few decades, there has been a tremendous increase in cancer biology data and treatment. Cancer research has opened exciting new areas of cellular and molecular biology. Month by month, new genes which regulate the carcinogenesis process are being discovered. The result is an incredible knowledge of cance...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.15.6.1213
更新日期:1999-12-01 00:00:00
abstract::Globally, breast cancer in women is the leading cause of cancer death. This fact has generated an interest to obtain insight into breast tumorigenesis and also to develop drugs to control the disease. Ras is a proto-oncogene that is activated as a response to extracellular signals. As a member of the Ras GTPase superf...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3298
更新日期:2016-02-01 00:00:00
abstract::Overexpression of either of the multidrug resistance genes MDR1 and MRP is associated with resistance of tumors to multiple chemotherapeutic agents. Overexpression of MDR1 has been reported in some cell lines derived from human hepatocellular carcinomas (HCC) and hepatoblastomas (HB). The human gene for cMOAT ( journal_title:International journal of oncology pub_type: 杂志文章 doi:10.3892/ijo.15.3.559 更新日期:1999-09-01 00:00:00
abstract::To investigate the usefulness of oral doxifluridine (5'-DFUR), an active intermediate metabolite of capecitabine (XELODA), in gastric cancer patients after curative resection, we conducted a phase III randomized controlled study to compare oral 5'-DFUR and oral 5-fluorouracil (5-FU). 485 gastric cancer patients with S...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3892/ijo.16.5.1021
更新日期:2000-05-01 00:00:00
abstract::Survivin is a member of the inhibitor of apoptosis protein (IAP) family and is frequently expressed in cancers, including meningiomas and gliomas. Survivin may be associated with tumor progression and poor prognosis of patients with brain tumors. Using ELISA and immunoblot analysis we asked whether survivin is capable...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::We have carried out three rounds of screening tests using 239 synthetic tripeptides for the growth-inhibitory activity toward tumor cells and found that those having amino acid sequences of Pro-Thr-Trp or Glu-Arg-Pro effectively inhibit proliferation of transformed cells. These peptides could be the fundamental struct...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::Specific RE protein species modulated by autocrine TGF alpha are unknown. HCT116 cells transfected with TGF alpha antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RE species. Nutrients alone (N) induced maximal mitog...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.6.1025
更新日期:1996-06-01 00:00:00
abstract::Quantitative nuclear morphometry (QNM) provides a reliable, reproducible method for measuring the degree of nuclear pleomorphism, a qualitative parameter widely incorporated into histopathological grading schemes. Nuclear shape has successfully been shown to predict primary prostate adenocarcinoma metastatic potential...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.3.673
更新日期:1995-09-01 00:00:00