Abstract:
:Optimal conditions have been defined to grow primary cultures of malignant melanoma suitable for in vitro pharmacological studies. A feasibility of primary cultures was observed in 60% of 62 clinical melanoma lymph node metastases. The neoplastic nature of the cells grown in culture, as assessed by the highly specific monoclonal antibodies anti-S100 and HMB45, was confirmed in 100% and 65% of the cases, respectively. Flow cytometric analysis showed a high stability of DNA content profiles observed in clinical samples through all the methodologic steps used to obtain in vitro cultures. The intertumor variability of the degree of the antiproliferative effect of melphalan and its relation with DNA interstrand cross-links (DNA ISC) provided preliminary evidence of the reliability of the experimental system for in vitro evaluation of anticancer drug activity.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Villa R,Zaffaroni N,Orlandi L,Costa A,Veronese S,Vaglini M,Silvestrini Rdoi
10.3892/ijo.1.6.619subject
Has Abstractpub_date
1992-11-01 00:00:00pages
619-24issue
6eissn
1019-6439issn
1791-2423journal_volume
1pub_type
杂志文章abstract::MicroRNAs (miRs) are short non-coding single stranded RNAs regulating the translation of target mRNAs in normal and cancer cells in which they are frequently dysregulated promoting tumor progression. Cancer stem cells (CSCs) of head and neck squamous cell carcinoma (HNSCC), identified by aldehyde-dehydrogenase express...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3142
更新日期:2015-10-01 00:00:00
abstract::Interleukin-2 (IL-2) which has no cross-resistance with chemotherapy, has given responses in tumors resistant to chemotherapy, and shown to be more effective when tumor burden is low. 13-cis retinoic acid (RA) has immunomodulatory properties, potentially synergistic with IL-2. The objective of this pilot phase II stud...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Since the efficacy of chemotherapy can be enhanced by targeting to specific receptors on tumors, we investigated the expression of LH-RH receptors in 5 human colon cancer lines and the effects of cytotoxic LH-RH analogs on these tumors. Nude mice bearing HT-29, HCT-116, HCT-15, LoVo and Colo-320DM cancers were treated...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-06-01 00:00:00
abstract::Although many chemotherapies have been developed for melanomas, successful therapy would be aided by the identification of intrinsic mechanisms that are crucial for melanoma survival. Here, we used resveratrol, a phytoalexin, as an anti-melanoma reagent. Applying resveratrol to various human and murine melanoma cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2480
更新日期:2014-08-01 00:00:00
abstract::Malignant pleural mesothelioma (MPM) is an aggressive cancer for which more effective treatments are needed. In this study, strong to moderate staining of MET and ERK5 was detected in 67.1 and 48% of the analyzed 73 human mesothelioma tumors, and significant correlation of MET and ERK5 expression was identified (P<0.0...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4021
更新日期:2017-07-01 00:00:00
abstract::Multidrug resistance (MDR) plays a pivotal role in human chronic myelogenous leukemia (CML) chemotherapy failure. MDR is mainly associated with the overexpression of drug efflux transporters of the ATP-binding cassette (ABC) proteins. Phosphoinositide 3-kinase (PI3K)/Akt signaling cascade is involved in the MDR phenot...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3738
更新日期:2016-12-01 00:00:00
abstract::Cancer stem cells (CSC) exhibit therapy resistance and drive self-renewal of the tumour, making cancer stem cells an important target for therapy. The PI3K signalling pathway has been the focus of considerable research effort, including in glioblastoma (GBM), a cancer that is notoriously resistant to conventional ther...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3510
更新日期:2016-07-01 00:00:00
abstract::Aurora kinase A (AURKA) regulates the cell cycle checkpoint and maintains genomic integrity. AURKA is overexpressed in various malignant tumors and its upregulation induces chromosomal instability, which leads to aneuploidy and cell transformation. To investigate the role of AURKA in endometrial cancer, we evaluated t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2842
更新日期:2015-04-01 00:00:00
abstract::The proteins of the rho gene family have been isolated from cytosolic as well as particulate fractions. The rho proteins have values of about 22KDa and have consensus amino acid sequences for GTP/GDP-binding and GTPase activities. We have localized the rho proteins in mammalian cells by using affinity-purified anti-pe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.6.1103
更新日期:1993-12-01 00:00:00
abstract::Clear cell renal cell carcinoma (ccRCC) is the most common renal malignancy in adults, the incidence of which continues to increase. The lipid droplet protein perilipin 2 (PLIN2), which was originally considered an RNA transcript, is markedly expressed during adipocyte differentiation. In addition, it has been observe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4384
更新日期:2018-07-01 00:00:00
abstract::The cell-adhesion properties of cancer cells can be targeted to block cancer metastasis. Although cytosolic lysyl-tRNA synthetase (KRS) functions in protein synthesis, KRS on the plasma membrane is involved in cancer metastasis. We hypothesized that KRS is involved in cell adhesion-related signal transduction for cell...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3381
更新日期:2016-04-01 00:00:00
abstract::Antagonists of growth hormone-releasing hormone (GH-RH) inhibit growth of various human cancers including osteosarcomas and Ewing's sarcomas, xenografted into nude mice or cultured in vitro. The antiproliferative effect of GH-RH antagonists could be mediated, in part, through the splice variants (SVs) of receptors for...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
abstract::Prognosis of patients with undifferentiated gastric cancer is generally poor. The expression of various microRNAs (miRNAs) has not been comprehensively investigated in undifferentiated gastric cancer. Total RNA was extracted from the specimens of 42 undifferentiated gastric cancer tissues and paired normal gastric tis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract::Induction of differentiation represents a promising concept for chemotherapy of malignant gliomas, which are often refractory even to the combined treatment with surgery, irradiation and chemotherapy. Since anti-neoplastic alkylphosphocholines can induce differentiation of leukemic cell lines, the effects of the intra...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.4.673
更新日期:2001-10-01 00:00:00
abstract::A431 resistant variants to epoxomicin (EXM) were established, showing 4.0-6.7 times more resistance to EXM than parental A431P. Both variants demonstrated increased expression of the beta-subunit molecules of 26S proteasome with approximately 2.5 times increased activity. In variant cells, cyclin B and P34cdc2 were ov...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::We analyzed the representation of CD44 isoforms with both exons v9 and v10 among CD44 total amount and also examined correlation between their expression, clinical parameters and survival. We used a semi-quantitative RT-PCR reaction and a panel of 25 normal breast specimens, 10 mammary fibroadenomas, 8 cystic samples ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.1.171
更新日期:1997-01-01 00:00:00
abstract::Telomerase activity and its subunits (hTERC, hTERT mRNA) were evaluated in ovarian tumours in relation to the expression of p53, c-Myc and estrogen receptor (ER). Furthermore, relations between telomerase activity, hTERC and hTERT with known clinicopathologic prognostic factors and survival in patients with malignant ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.23.5.1451
更新日期:2003-11-01 00:00:00
abstract::In this study tumor-specific CTL effector and memory responses were analyzed in normal mice, in tumor bearing mice and in animals treated by active specific immunotherapy (ASI) with an autologous virus modified tumor vaccine. In the well defined DBA/2 mouse lymphoma model ESb, the tumor specific CTL response requires ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.2.217
更新日期:1999-08-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) has four isoforms (VEGF121, VEGF165, VEGF189, VEGF206). We examined the expression patterns of VEGF isoforms in non-small cell lung cancer (NSCLC) by RT-PCR. The cell-associated isoform VEGF189 was more frequently expressed in NSCLC (90.5%) than in extraneoplastic lung tissue ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.3.541
更新日期:1998-03-01 00:00:00
abstract::Frizzled (FZD) genes encode receptors for WNTs, which play key roles in carcinogenesis and embryogenesis. We have previously cloned the FZD10 gene, and demonstrated up-regulation of FZD10 mRNA in the cervical cancer cell line HeLa S3, gastric cancer cell lines TMK1 and MKN74, and 4 cases out of 10 cases of primary gas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Gene amplification is a frequent event in lung cancer, specifically in squamous cell lung carcinoma. Recently, we reported amplifications on chromosomal bands 3q26.1-q26.3 with the genes BCHE and SLC2A2 amplified in 40% of squamous cell lung carcinomas. Here, we identified an amplified domain within chromosomal bands ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.1.67
更新日期:2000-07-01 00:00:00
abstract::Currently, some evidence suggests that human multipotential mesenchymal stems cells (hMSCs) aid tumor growth and metastasis. Nutrient deprivation and oxygen deficiency are representative characteristics of solid tumor microenvironment during the cancer development. Because the effects of hMSCs on tumors under stressfu...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1810
更新日期:2013-04-01 00:00:00
abstract::Human malignant neuroblastoma is characterized by poor differentiation and uncontrolled proliferation of immature neuroblasts. Retinoids such as all-trans-retinoic acid (ATRA), 13-cis-retinoic acid (13-CRA), and N-(4-hydroxyphenyl) retinamide (4-HPR) at low doses are capable of inducing differentiation, while flavonoi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000201
更新日期:2009-03-01 00:00:00
abstract::Transforming growth factor-β receptor II (TGFBR2) is implicated in various types of cancer. Most molecules involved in the TGF-β pathway can be degraded by one or more microRNAs (miRNAs). In the present study, we show that miRNA plays an important role in downregulating TGFBR2 expression in Ewing's sarcoma (ES) cells....
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4155
更新日期:2017-12-01 00:00:00
abstract::The intensity of post-treatment melanoma patient follow-up varies widely among physicians. We investigated whether physician age accounts for the observed variation in surveillance intensity among plastic surgeons. A custom-designed questionnaire was mailed to USA and non-USA surgeons, all of whom were members of the ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.1.175
更新日期:2001-07-01 00:00:00
abstract::Baicalein is a flavonoid derived originally from the root of Scutellaria baicalensis Georgi, which has been used in Oriental medicines for treating various diseases. Although this compound has been reported to have anticancer activities in several human cancer cell lines, the therapeutic effects of baicalein on human ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3606
更新日期:2016-09-01 00:00:00
abstract::Cadherins play a crucial role in epithelial morphogenesis and mediate intercellular adhesion. These receptors bind catenins and are involved in signal transduction pathways that regulate cell growth and apoptosis, and are frequently down-regulated in invasive and metastatic carcinomas. In order to assess the role of E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Gap junctional intercellular communication (GJIC) has been shown to be involved in the bystander effect through herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) gene therapy. In this study, we examined the expression of connexins, the components of gap junction, and the degree of GJIC in esophageal cance...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::The most common therapeutic approach for many cancers is chemotherapy. However, many patients relapse after treatment due to the development of chemoresistance. Recently, targeted therapies represent novel approaches to destroy cancer cells. The PI3K/PTEN/AKT pathway is a key signal...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2011.1312
更新日期:2012-03-01 00:00:00
abstract::Chemokine-mediated activation of G protein-coupled receptors CXCR1/2 promotes tumor growth, invasion, inflammation and metastasis. Repertaxin, a CXCR1/2 small-molecule inhibitor, has been shown to attenuate many of these tumor-associated processes. The present study aimed to investigate the effects of repertaxin alone...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3371
更新日期:2016-04-01 00:00:00