Abstract:
:Erythropoietin (EPO), the key hormone for erythropoiesis, also increases nitric oxide (NO) bioavailability in endothelial cells (ECs), yet the definitive mechanisms are not fully understood. Increasing evidence has demonstrated that β common receptor (βCR) plays a crucial role in EPO-mediated non-hematopoietic effects. We investigated the role of βCR in EPO-induced endothelial NO synthase (eNOS) activation in bovine aortic ECs (BAECs) and the molecular mechanisms involved. Results of confocal microscopy and immunoprecipitation analyses revealed that βCR was colocalized and interacted with EPO receptor (EPOR) in ECs. Inhibition of βCR or EPOR by neutralizing antibodies or small interfering RNA abolished the EPO-induced NO production. Additionally, blockage of βCR abrogated the EPO-induced increase in the phosphorylation of eNOS, Akt, Src, or Janus kinase 2 (JAK2). Immunoprecipitation analysis revealed that treatment with EPO increased the interaction between βCR and eNOS, which was suppressed by inhibition of Src, JAK2, or Akt signaling with specific pharmacological inhibitors. Furthermore, EPO-induced EC proliferation, migration, and tube formation were blocked by pretreatment with βCR antibody and Src, JAK2, or PI3K/Akt inhibitors. Moreover, in vivo experiments showed that EPO increased the level of phosphorylated eNOS, Src, JAK2, and Akt, as well as βCR-eNOS association in aortas and promoted the angiogenesis in Matrigel plug, which was diminished by βCR or EPOR neutralizing antibodies. Our findings suggest that βCR may play an integrative role in the EPO signaling-mediated activation of eNOS in ECs.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Su KH,Shyue SK,Kou YR,Ching LC,Chiang AN,Yu YB,Chen CY,Pan CC,Lee TSdoi
10.1002/jcp.22678subject
Has Abstractpub_date
2011-12-01 00:00:00pages
3330-9issue
12eissn
0021-9541issn
1097-4652journal_volume
226pub_type
杂志文章abstract::As a natural metabolite of limonoids from Dictamnus dasycarpus, fraxinellone has been reported to be neuroprotective and anti-inflammatory. However, its influence on cellular metabolism remains largely unknown. In the present study, we investigated the effect of fraxinellone on cellular senescence-induced by oxidative...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26169
更新日期:2018-05-01 00:00:00
abstract::Overexpression of IGF-1 in C2C12 myoblasts causes hypertrophy when myoblasts fuse to form myotubes, a response that requires elevated intracellular calcium. We show that myoblasts contain a store-operated Ca2+ channel (SOCC) whose activity is enhanced with IGF-1 overexpression. A membrane protein, CD20, can cause Ca2+...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10347
更新日期:2003-10-01 00:00:00
abstract::The presence of an appropriate number of viable cells is prerequisite for successive differentiation during chondrogenesis. Chondrogenic differentiation has been reported to be influenced by mechanical stimuli. This research aimed to study the effects of cyclic compressive stress on cell viability of rat bone marrow-d...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24359
更新日期:2013-09-01 00:00:00
abstract::Diabetic nephropathy (DN) is one of the most serious and dangerous chronic complications caused by diabetes mellitus, and the identification and development of novel biomarkers could be beneficial for the diagnosis and prognosis of DN patients. This study focused on researching the differently expressed pattern of the...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.26958
更新日期:2019-02-01 00:00:00
abstract::Addition of bombesin in the presence of either forskolin or cholera toxin caused a marked (4-6 fold) enhancement of cAMP accumulation in Swiss 3T3 cells. This effect was time and concentration dependent, induced by various bombesin-like peptides and blocked by a bombesin antagonist. Enhancement of cAMP accumulation by...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041370203
更新日期:1988-11-01 00:00:00
abstract::3-Hydroxy 3-methylglutaryl coenzyme A reductase (HMG-CoAR) is the key and rate-limiting enzyme of cholesterol biosynthetic pathway. Although HMG-CoAR activity has already been related to the differentiation of some cellular lines there are no studies that analyze the role of HMG-CoAR, and the pathway it is involved wi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21810
更新日期:2009-08-01 00:00:00
abstract::Gicerin/CD146 is a cell adhesion molecule, which belongs to the immunoglobulin (Ig) superfamily. We have reported that it has a homophilic binding activity, which participates in the neurite extension from embryonic neurons. To elucidate how gicerin is involved in the neurite extension mechanism, we employed PC12 cell...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20365
更新日期:2005-08-01 00:00:00
abstract::Curcumin is known to be a potent wound healer. Despite this, studies on curcumin using certain model systems have shown it to be anti-angiogenic. Results of the present investigations suggest that curcumin causes opposing effects on angiogenesis in serum stimulated and unstimulated conditions. The evidence in support ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21307
更新日期:2008-04-01 00:00:00
abstract::Recent evidence has verified the cardioprotective actions of irisin in different diseases models. However, the beneficial action of irisin on hypoxia-reoxygenation (HR) injury under high glucose stress has not been described. Herein our research investigated the influence of irisin on HR-triggered cardiomyocyte death ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29030
更新日期:2020-02-01 00:00:00
abstract::Priapism, a prolonged penile erection in the absence of sexual arousal, is common among patients with sickle cell disease (SCD). Hypogonadism is also common in patients with SCD. While the administration of exogenous testosterone reverses hypogonadism, it is contraceptive. We hypothesized that the stimulation of endog...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.30075
更新日期:2020-09-24 00:00:00
abstract::Protease nexin-I (PN-I, Mr approximately 43,000) is representative of a newly described class of cell-secreted protease inhibitors. PN-I has been purified to apparent homogeneity, partially sequenced, and monospecific antibodies have been raised against it. PN-I is a potent inhibitor of urokinase, thrombin, plasmin, a...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041320217
更新日期:1987-08-01 00:00:00
abstract::The main aim of this investigation was to determine whether a functional relationship existed between epidermal growth factor (EGF) and voltage-gated sodium channel (VGSC) upregulation, both associated with strongly metastatic prostate cancer cells. Incubation with EGF for 24 h more than doubled VGSC current density. ...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21289
更新日期:2008-04-01 00:00:00
abstract::Mouse fetuses at 15th and 18th day of gestation, and newborns aging 0, 5, 10, and 15 days were injected i.p. with 131 I-albumin (RISA). The degree of incorporation by liver and the intraparticulate hydrolysis of RISA in 27,000g x 10 min. particles increased after birth. By this time, changes in subcellular distributio...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041090211
更新日期:1981-11-01 00:00:00
abstract::Cyclosporine A (CyA) is a powerful nonsteroidal immunosuppressive agent used to prevent graft rejection of organ and bone marrow transplants. A major side effect observed can be attributed to the fibroblast and its functions: proliferation of fibroblasts and formation of fibrotic tissue in the gingiva (fibrous hyperpl...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041520222
更新日期:1992-08-01 00:00:00
abstract::Vascular endothelial cells receive proangiogenic or antiangiogenic signals from components of extracellular matrix (ECM) depending upon the situation and many molecular signals can have opposite effects in different vascular beds. Tissue inhibitor of metalloproteinase 1 is antiangiogenic in several tissues, but promot...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20645
更新日期:2006-07-01 00:00:00
abstract::Copper, an essential trace element, can be toxic to some cells when present in excess. But thorough investigations into the cytotoxicity of copper and subsequent molecular mechanisms are rare, although the cytotoxicity of copper has been applied to cancer chemotherapy. The present study demonstrates that Cu(2+) inhibi...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/1097-4652(200008)184:2<161::AID-JCP3>3.0.C
更新日期:2000-08-01 00:00:00
abstract::Non-small-cell lung cancer (NSCLC) remains the leading cause of cancer death worldwide. As a platinum-based chemotherapeutic drug, cisplatin has been used for over 30 years in NSCLC treatment while its effects are diminished by drug resistance. Therefore, we aimed to study the potential role of UCA1 in the development...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29266
更新日期:2020-04-01 00:00:00
abstract::This study aimed at investigating the expression and function of uracil nucleotide-sensitive receptors (P2Y(2), P2Y(4), and P2Y(6)) on osteogenic differentiation of human bone marrow stromal cells (BMSCs) in culture. Bone marrow specimens were obtained from postmenopausal female patients (68 ± 5 years old, n = 18) und...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.23014
更新日期:2012-06-01 00:00:00
abstract::Evidence is emerging that long noncoding RNAs (lncRNAs) play vital roles in tumorigenesis. LncRNA gastric cancer-associated transcript 3 (GACAT3) is reported to participate in the development of breast cancer, colorectal cancer, nonsmall cell lung cancer, and gastric cancer. However, whether it is implicated in glioma...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.27542
更新日期:2019-06-01 00:00:00
abstract::Topical intranasal application of the antifungal Amphotericin B (AmphoB) has been shown as an effective medical treatment of chronic rhinosinusitis. Because this antibiotic forms channels in lipid membranes, we considered the possibility that it affects the properties and/or cell surface expression of ion channels/pum...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20347
更新日期:2005-09-01 00:00:00
abstract::The C-terminal region of parathyroid hormone-related protein (PTHrP) containing the sequence (107-111) appears to be a potent inhibitor of osteoclastic bone resorption. In the present study, we have investigated the effect of human (h)PTHrP (107-139) and hPTHrP (107-111)NH2 on the proliferation of osteoblastic rat ost...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1
更新日期:1997-02-01 00:00:00
abstract::The epithelium-specific ETS (ESE)-1 transcription factor is induced in chondrocytes by interleukin-1beta (IL-1beta). We reported previously that early activation of EGR-1 by IL-1beta results in suppression of the proximal COL2A1 promoter activity by displacement of Sp1 from GC boxes. Here we report that ESE-1 is a pot...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.21338
更新日期:2008-05-01 00:00:00
abstract::Acute lung injury (ALI) is a severe acute inflammatory reaction of the lungs caused by a variety of factors, which can lead to a high mortality rate. MicroRNAs are a novel therapeutic molecule that play a vital role in many diseases. However, its mechanism of action in lipopolysaccharide (LPS)-induced mouse ALI is not...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.28850
更新日期:2019-12-01 00:00:00
abstract::The exposure to non-thermal microwave electromagnetic field (MW-EMF) at 1.95 MHz, a frequency used in mobile communication, affects the refolding kinetics of eukaryotic proteins (Mancinelli et al., 2004). On these basis we have evaluated the in vivo effect of MW-EMF in human epidermoid cancer KB cells. We have found t...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.20327
更新日期:2005-08-01 00:00:00
abstract::The Schneider membrane is the mucosa that covers the inner part of the maxillary sinus cavities. The free surface is a ciliated pseudostratified epithelium, while the deeper portion is a highly vascularized connective tissue. The stromal fraction, bordering the bony wall of the sinus, after tooth loss can exhibit incr...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24022
更新日期:2012-09-01 00:00:00
abstract::Hyperinsulinemia is a likely cause of the increased cancer incidence and mortality in diabetic patients, but its role is difficult to define in vivo. Previous in vitro studies testing the mitogenic potential of insulin and its analogs provided incomplete and sometimes contradictory results. To better evaluate cancer c...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.24635
更新日期:2014-11-01 00:00:00
abstract::Tight junctions govern the paracellular permeability of endothelial and epithelial cells. Aberrations of tight junction function are an early and key event during the vascular spread of cancer and inflammation. This study sought to determine the role of estrogen in the regulation of tight junctions and expression of m...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.10315
更新日期:2003-08-01 00:00:00
abstract::Considering the high rate of osteoclast-related diseases worldwide, research targeting osteoclast formation/function is crucial. In vitro, we demonstrated that chitooligosaccharide (CS) dramatically inhibited osteoclastogenesis as well as osteoclast function dose-dependently. CS suppressed osteoclast-specific genes ex...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.29207
更新日期:2020-03-01 00:00:00
abstract::Ischemic stroke (IS), which is characterized by high morbidity, disability, and mortality, is recognized as a major cerebrovascular disease. MicroRNA-31 (miR-31) was reported to participate in the progression of brain disease. The present study was conducted in order to investigate the effect of miR-31 on oxidative st...
journal_title:Journal of cellular physiology
pub_type: 杂志文章,收录出版
doi:10.1002/jcp.29146
更新日期:2020-03-01 00:00:00
abstract::When calcium is removed from culture medium, motility of cultured cells is decreased. The effect is rapid, reversible and pronounced. Decreased motility is observed with normal mouse Balb/c 3T3 cells, mouse L929 cells, rat kidney fibroblasts and chick embryo fibroblasts. The calcium dependence of movement can be obser...
journal_title:Journal of cellular physiology
pub_type: 杂志文章
doi:10.1002/jcp.1041010112
更新日期:1979-10-01 00:00:00