Abstract:
:The C-terminal region of parathyroid hormone-related protein (PTHrP) containing the sequence (107-111) appears to be a potent inhibitor of osteoclastic bone resorption. In the present study, we have investigated the effect of human (h)PTHrP (107-139) and hPTHrP (107-111)NH2 on the proliferation of osteoblastic rat osteosarcoma UMR 106 cells. We found that both C-terminal PTHrP peptides, like hPTHrP (1-141), were antimitogenic for these cells, between 1 pM and 10 nM. [Tyr34]hPTHrP (1-34)NH2 was as potent as these peptides but less effective as growth inhibitor in these cells. UMR 106 cells were found to produce and secrete immunoreactive PTHrP. Addition of anti-PTHrP neutralizing antibodies to C- and N-terminal epitopes of PTHrP increased the growth of these cells. Our data suggest that the antiproliferative effect of these C-terminal PTHrP analogs may be independent of cyclic adenosine 3':5'-monophosphate (cAMP) and mediated by protein kinase C. These findings support an autocrine role of PTHrP in bone metabolism.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Valín A,García-Ocaña A,De Miguel F,Sarasa JL,Esbrit Pdoi
10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1subject
Has Abstractpub_date
1997-02-01 00:00:00pages
209-15issue
2eissn
0021-9541issn
1097-4652pii
10.1002/(SICI)1097-4652(199702)170:2<209::AID-JCP1journal_volume
170pub_type
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