Involvement of glutathione in the inhibition of sea urchin egg mitosis by phenyl glyoxal.

Abstract:

:Cell division in fertilized sea urchin eggs was reversibly inhibited when the ketoaldehyde phenyl glyoxal (PG) at a concentration of 0.1 mM was added to eggs for ten minutes prior to the formation of the mitotic spindle. We investigated whether inhibition of mitosis was due to PG binding to the cell surface (as previously suggested by Stein and Berestecky, '74) or to some intracellular effect. When 14C-PG was added to eggs, label was readily taken up into the egg cytoplasm; very little label was associated with the egg surface. In the cytoplasm PG combined with equimolar amounts of reduced glutathione (GSH), decreasing the levels of cellular GSH to less than 15% of normal and accounting for at least 50% of the PG taken up by eggs. The concentrations of oxidized and protein-bound glutathione were unaffected by PG treatment. We showed that glyoxalase enzymes were present in sea urchin eggs and were capable of metabolizing the PG-GSH complex, thereby restoring GSH to normal levels after PG was removed from the sea water. Though some other effect of PG cannot be ruled out, the major fate of PG in eggs was to combine with GSH, and the transient decrease in GSH which resulted could lead to inhibition of mitosis. While other reports (Nath and Rebhun, '76; Oliver et al., '76) have shown that reagents which oxidize GSH disrupt microtubule-related events, our results showed that such inhibition could be caused by decreased GSH levels alone.

journal_name

J Cell Physiol

authors

Amy CM,Rebhun LI

doi

10.1002/jcp.1041000119

subject

Has Abstract

pub_date

1979-07-01 00:00:00

pages

187-98

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

100

pub_type

杂志文章
  • Characterization of cellular and humoral immune responses to alkaline phosphatase from fertile hydatid cysts in the human peripheral blood.

    abstract::Hydatid cyst, the larval stage of the tapeworm Echinococcus granulosus and a causative agent of cystic echinococcosis, possesses a vast number of antigenic peptides that are constantly presented in the host immune system during infection. Here, we sought to provide more information about the cellular/humoral component...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.27092

    authors: Vatankhah A,Ghaffari SMR,Vatankhah RG,Piurkó V,Tímár J,Avan A,Jazayeri MH

    更新日期:2019-03-01 00:00:00

  • The effects and mechanisms of myeloid differentiation protein 2 on intestinal mucosal permeability in mice with chronic colitis.

    abstract::The present study was designed to investigate the mechanism of myeloid differentiation protein 2 (MD2) on intestinal mucosa destruction in mice with chronic colitis. Briefly, a chronic colitis mouse model was established by the administration of dextran sulfate sodium (DSS) in transgenic mice of MD2 overexpression (Tr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28711

    authors: Han C,Guan Q,Guo L,Yang Y,Ruan S,Zhang X

    更新日期:2019-11-01 00:00:00

  • Isolation and characterization of a cloned growth factor dependent macrophage cell line, BAC1.2F5.

    abstract::The SV40 transformed murine macrophage cell line, BAC1, proliferates in response to the colony stimulating factor, CSF-1 (Schwarzbaum et al., J. Immunol., 132:1158, 1984). In order to obtain a cell line suitable for biochemical and genetic studies of CSF-1 signal transduction, clones of BAC1 were established. Clones r...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041300316

    authors: Morgan C,Pollard JW,Stanley ER

    更新日期:1987-03-01 00:00:00

  • β2-Adrenoreceptor-Mediated Proliferation Inhibition of Embryonic Pluripotent Stem Cells.

    abstract::Adrenoreceptors (ARs) are widely expressed and play essential roles throughout the body. Different subtype adrenoceptors elicit distinct effects on cell proliferation, but knowledge remains scarce about the subtype-specific effects of β2-ARs on the proliferation of embryonic pluripotent stem (PS) cells that represent ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24937

    authors: Sun F,Yang XJ,Lv HY,Tang YB,An SM,Ding XP,Li WB,Teng L,Shen Y,Chen HZ,Zhu L

    更新日期:2015-11-01 00:00:00

  • Expression of parathyroid hormone-related protein in cells of osteoblast lineage.

    abstract::The expression of parathyroid hormone-related protein (PTHrP) was studied in a range of cell cultures representative of the osteoblast lineage and in rat calvarial sections. Primary newborn rat calvarial cells, a rat preosteoblastic cell line (UMR 201), a mouse stromal cell line (ST 2), a mouse calvaria-derived osteob...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199601)166:1<94::AID-JCP11

    authors: Suda N,Gillespie MT,Traianedes K,Zhou H,Ho PW,Hards DK,Allan EH,Martin TJ,Moseley JM

    更新日期:1996-01-01 00:00:00

  • Inhibition of chemotactic factor-induced neutrophil responsiveness by arachidonic acid.

    abstract::Arachidonic acid when added simultaneously with the chemotactic peptide formyl-methionyl-leucyl-phenylalanine (f-Met-Leu-Phe) inhibits the ability of the latter to initiate several but not all of its effects on rabbit peritoneal neutrophils. Stimulated neutrophil aggregation, calcium uptake, and increases in the stead...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041150305

    authors: Naccache PH,Molski TF,Volpi M,Mackin WM,Becker EL,Sha'afi RI

    更新日期:1983-06-01 00:00:00

  • Versican modulates gap junction intercellular communication.

    abstract::Versican is a large chondroitin sulfate proteoglycan and belongs to the family of lecticans. Versican possesses two globular domains, G1 and G3 domain, separated by a CS-attachment region. The CS-attachment region present in the middle region is divided into two spliced domains named CSalpha and beta. Alternative spli...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20921

    authors: Sheng W,Dong H,Lee DY,Lu WY,Yang BB

    更新日期:2007-04-01 00:00:00

  • Induction of microvillar hydrolase activities by cell density and exogenous differentiation inducers in an established kidney epithelial cell line (LLC-PK1).

    abstract::Several hydrolase activities characteristic of the apical brush border membrane of renal proximal tubule, leucine aminopeptidase, gamma-glutamyl transpeptidase, alkaline phosphatase, maltase, and trehalase, were identified in cultures of the LLC-PK1 kidney epithelial cell line. A coordinate increase in activities of t...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041210109

    authors: Yoneyama Y,Lever JE

    更新日期:1984-10-01 00:00:00

  • C1q/tumor necrosis factor-related protein-6 attenuates TNF-α-induced apoptosis in salivary acinar cells via AMPK/SIRT1-modulated miR-34a-5p expression.

    abstract::C1q/tumor necrosis factor-related protein-6 (CTRP6) is a newly identified adipokine involved in diverse biological processes. However, its role in salivary glands remains unknown. Here, we demonstrated that CTRP6 was mainly distributed in the nuclei, apicolateral membranes, and cytoplasm of human submandibular glands ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.30262

    authors: Qu LH,Hong X,Zhang Y,Cong X,Xiang RL,Mei M,Su JZ,Wu LL,Yu GY

    更新日期:2021-01-05 00:00:00

  • Hyperthermia inhibits homologous recombination repair and sensitizes cells to ionizing radiation in a time- and temperature-dependent manner.

    abstract::Hyperthermia has long been known as a radio-sensitizing agent that displays anti-tumor effects, and has been developed as a therapeutic application. The mechanisms of hyperthermia-induced radio-sensitization are highly associated with inhibition of DNA repair. Our investigations aimed to show how hyperthermia inactiva...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24302

    authors: Genet SC,Fujii Y,Maeda J,Kaneko M,Genet MD,Miyagawa K,Kato TA

    更新日期:2013-07-01 00:00:00

  • Thrombin activates mitogen-activated protein kinase in primary astrocyte cultures.

    abstract::Thrombin is known to evoke numerous inflammatory and proliferative responses in a wide variety of its target cells. Recent studies have demonstrated morphoregulatory and mitogenic effects of thrombin on astroglial cells (astrocytes). The present study deals with thrombin-induced activation of mitogen-activated protein...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041650223

    authors: Bhat NR,Zhang P,Hogan EL

    更新日期:1995-11-01 00:00:00

  • COMMD7 as a novel NEMO interacting protein involved in the termination of NF-κB signaling.

    abstract::NEMO/IKKγ is the regulatory subunit of the IκB Kinase (IKK) complex, required for the activation of the NF-κB pathway, which is involved in a variety of key processes, including immunity, inflammation, differentiation, and cell survival. Termination of NF-κB activity on specific -κB responsive genes, which is crucial ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25066

    authors: Esposito E,Napolitano G,Pescatore A,Calculli G,Incoronato MR,Leonardi A,Ursini MV

    更新日期:2016-01-01 00:00:00

  • High glucose alters the secretome of mechanically stimulated osteocyte-like cells affecting osteoclast precursor recruitment and differentiation.

    abstract::Diabetes mellitus (DM) induces bone deterioration, while mechanical stimulation promotes osteocyte-driven bone formation. We aimed to evaluate the interaction of acute exposure (24 h) to high glucose (HG) with both the pro-survival effect conferred to osteocytic MLO-Y4 cells and osteoblastic MC3T3-E1 cells by mechanic...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25829

    authors: Maycas M,Portolés MT,Matesanz MC,Buendía I,Linares J,Feito MJ,Arcos D,Vallet-Regí M,Plotkin LI,Esbrit P,Gortázar AR

    更新日期:2017-12-01 00:00:00

  • Regulation of cerebroside sulfotransferase activity in cultured oligodendrocytes: effect of growth factors and insulin.

    abstract::Cerebroside sulfotransferase (EC 2.8.2.11, CST) specific activity has been determined in oligodendrocyte (OL)-enriched glial cell cultures from newborn rat brain grown in serum supplemented medium. This activity is detectable at 5 days in vitro (DIV) and reaches its maximum value at 12 DIV. This period corresponds to ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041410327

    authors: Fressinaud C,Sarliève LL,Labourdette G

    更新日期:1989-12-01 00:00:00

  • Mesenchymal stem cells contribute to endogenous FVIII:c production.

    abstract::Besides the liver, it has been difficult to identify which organ(s) and/or cellular component(s) contribute significantly to the production of human FVIII:c (FVIII). Thus far, only endothelial cells have been shown to constitute a robust extrahepatic source of FVIII, possibly explaining both the diverse presence of FV...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24247

    authors: Sanada C,Kuo CJ,Colletti EJ,Soland M,Mokhtari S,Knovich MA,Owen J,Zanjani ED,Porada CD,Almeida-Porada G

    更新日期:2013-05-01 00:00:00

  • Second messenger signaling of c-fos gene induction by parathyroid hormone (PTH) and PTH-related peptide in osteoblastic osteosarcoma cells: its role in osteoblast proliferation and osteoclast-like cell formation.

    abstract::The present study was performed to clarify second messenger signaling in parathyroid hormone (PTH)-induced c-fos gene expression, to characterize the participation of the c-fos gene in the regulation of osteoblast proliferation and function as well as osteoclast-like cell formation by PTH and to compare these effects ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041610221

    authors: Kano J,Sugimoto T,Kanatani M,Kuroki Y,Tsukamoto T,Fukase M,Chihara K

    更新日期:1994-11-01 00:00:00

  • Studies on the regulation of the three enzymes of the Leloir pathway in cultured mammalian cells. II. A search for quantitative interrelationships between the three enzyme activities.

    abstract::Studies on a normal human diploid cell strain revealed that the specific activity of the cell protein, for each of the three enzymes of the Leloir pathway, changed significantly as the cells grew. The kinetics of change in specific activity varied according to the enzyme being studied, and the kinetics for each enzyme...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040860112

    authors: Stern ES,Krooth RS

    更新日期:1975-08-01 00:00:00

  • The effects of Clostridium perfringens enterotoxin on morphology, viability, and macromolecular synthesis in Vero cells.

    abstract::Vero (African green monkey kidney) cells grown in tissue culture monolayer were sensitive to Clostridium perfringens enterotoxin. Within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. Nearly half of the cells were non...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040990205

    authors: McClane BA,McDonel JL

    更新日期:1979-05-01 00:00:00

  • Transmembrane potential responses during HL-60 promyelocyte differentiation.

    abstract::Myeloid cells, including granulocytes and monocyte/macrophages, are important in disease-associated inflammatory reactions. These cells come from a common progenitor, the promyelocyte. The human promyelocytic cell line, HL-60, can be induced to terminally differentiate into granulocytes or monocyte/ macrophages in a c...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199607)168:1<155::AID-JCP1

    authors: Brent LH,Rubenstein B,Gong QH,Wieland SJ

    更新日期:1996-07-01 00:00:00

  • Regulation of mesangial cell Na+/Ca2+ exchanger isoforms.

    abstract::An isoform of the Na(+)/Ca(2+) exchanger (SDNCX1.10) was cloned from mesangial cells of Sprague-Dawley rat. Regulation of this isoform was compared to two other clones that were derived from the Dahl/Rapp salt sensitive (SNCX) and salt resistant rat (RNCX). All isoforms differ at the alternative splice site and at ami...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10447

    authors: Williams I,Williams C,Siroky B,Bates E,Kovacs G,Peti-Peterdi J,Unlap MT,Bell PD

    更新日期:2004-05-01 00:00:00

  • Diacylglycerols and mezerein activate neutrophils by a phorbol myristate acetate-like mechanism.

    abstract::rac-1-O-Myristoyl-2-O-acetylglycerol, rac-1-O-palmitoyl-2-O-acetylglycerol, and rac-1-O-oleoyl-2-O-acetylglycerol acted like phorbol myristate acetate and mezerein in stimulating human neutrophil aggregation. Responses to these agents were equally influenced by cytochalasin B, extracellular calcium and magnesium, arac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041250204

    authors: O'Flaherty JT,Schmitt JD,Wykle RL,Redman JF Jr,McCall CE

    更新日期:1985-11-01 00:00:00

  • Temperature dependent expression of polyoma virus in murine embryonal carcinoma cells.

    abstract::At 37 degrees C, undifferentiated murine teratocarcinoma cells (PCC4) are resistant to infection with SV40 and Polyoma virus. When infection is carried out at 31 degrees C, these cells become fully susceptible to a variety of polyoma virus strains, including wt, ts-a, and hr-t; they also display an increased susceptib...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041050104

    authors: Dandolo L,Vasseur M,Kress C,Aghion J,Blangy D

    更新日期:1980-10-01 00:00:00

  • Decreased in vivo and in vitro colony stimulating activity responses to bacterial lipopolysaccharide in C3H/HeJ mice.

    abstract::Mice of the C3H/HeJ strain exhibit low inflammatory and immunological responses to certain bacterial lipopolysaccharide (LPS) preparations. Lymphocytes from C3H/HeJ mice also show defective LPS-induced mitogenesis. We tested the colony stimulating activity (CSA) response of C3H/HeJ mice. As controls we used mice of th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1040920219

    authors: Russo M,Lutton JD

    更新日期:1977-08-01 00:00:00

  • ELP2 negatively regulates osteoblastic differentiation impaired by tumor necrosis factor α in MC3T3-E1 cells through STAT3 activation.

    abstract::Tumor necrosis factor-α (TNF-α) is a pluripotent signaling molecule. The biological effect of TNF-α includes slowing down osteogenic differentiation, which can lead to bone dysplasia in long-term inflammatory microenvironments. Signal transducer and activator of transcription 3 (STAT3)-interacting protein 1 (StIP1, al...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28440

    authors: Xu CP,Sun HT,Yang YJ,Cui Z,Wang J,Yu B,Wang FZ,Yang QP,Qi Y

    更新日期:2019-08-01 00:00:00

  • A cloning assay for 6-thioguanine resistance provides evidence against certain somatic mutational theories of aging.

    abstract::The frequencies of 6-thioguanine-resistant primary clones from the kidneys and skeletal muscles of aging male cohorts of two F1 hybrid strains of Mus musculus varied from 0.59 to 10.96 X 10(-5) and did not increase as a function of donor age (up to 40 months). Resistant clones were shown to be severely deficient in th...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041210207

    authors: Horn PL,Turker MS,Ogburn CE,Disteche CM,Martin GM

    更新日期:1984-11-01 00:00:00

  • Transforming growth factor beta inhibits plasminogen activator (PA) activity and stimulates production of urokinase-type PA, PA inhibitor-1 mRNA, and protein in rat osteoblast-like cells.

    abstract::Transforming growth factor beta (TGF beta) treatment of rat osteoblast-rich calvarial cells or of the clonal osteogenic sarcoma cells, UMR 106-01, resulted in dose-dependent inhibition of plasminogen activator (PA) activity, and increased production of 3.2 kb mRNA and protein for PA inhibitor -1 (PAI-1). Although tiss...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041490106

    authors: Allan EH,Zeheb R,Gelehrter TD,Heaton JH,Fukumoto S,Yee JA,Martin TJ

    更新日期:1991-10-01 00:00:00

  • Selenium deficiency in cultured adrenocortical cells: restoration of glutathione peroxidase and resistance to hydroperoxides on addition of selenium.

    abstract::Cultured bovine adrenocortical cells were previously shown to be functionally deficient in selenium and vitamin E when grown in medium supplemented with fetal bovine serum. In the present experiments, the lack of significant bioavailable amounts of selenium in the medium was demonstrated by the finding of only low lev...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041230106

    authors: Hornsby PJ,Pearson DW,Autor AP,Aldern KA,Harris SE

    更新日期:1985-04-01 00:00:00

  • Tranexamic acid suppresses the release of mitochondrial DNA, protects the endothelial monolayer and enhances oxidative phosphorylation.

    abstract::Damage-associated molecular patterns, including mitochondrial DNA (mtDNA) are released during hemorrhage resulting in the development of endotheliopathy. Tranexamic acid (TXA), an antifibrinolytic drug used in hemorrhaging patients, enhances their survival despite the lack of a comprehensive understanding of its cellu...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.28603

    authors: Prudovsky I,Carter D,Kacer D,Palmeri M,Soul T,Kumpel C,Pyburn K,Barrett K,DeMambro V,Alexandrov I,Brandina I,Kramer R,Rappold J

    更新日期:2019-11-01 00:00:00

  • Presence of MUC4 in human milk and at the luminal surfaces of blood vessels.

    abstract::MUC4 is a heterodimeric membrane mucin, composed of a mucin subunit ASGP-1 (MUC4alpha) and a transmembrane subunit ASGP-2 (MUC4beta), which has been implicated in the protection of epithelial cell surfaces. Surprisingly, development and characterization of a new monoclonal antibody (mAb), called 1G8, against ASGP-2 de...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20277

    authors: Zhang J,Perez A,Yasin M,Soto P,Rong M,Theodoropoulos G,Carothers Carraway CA,Carraway KL

    更新日期:2005-07-01 00:00:00

  • SOCS-2 interferes with myotube formation and potentiates osteoblast differentiation through upregulation of JunB in C2C12 cells.

    abstract::Suppressor of cytokine signaling (SOCS)-2 regulates normal postnatal growth and its deficiency in mice causes gigantism with increased bone length and proportional enlargement in skeletal muscles. Using C2C12 mesenchymal precursor cell line as a model, we investigated a possible role of SOCS-2 in the differentiation p...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20579

    authors: Ouyang X,Fujimoto M,Nakagawa R,Serada S,Tanaka T,Nomura S,Kawase I,Kishimoto T,Naka T

    更新日期:2006-05-01 00:00:00