Protein kinase C can inhibit TRPC3 channels indirectly via stimulating protein kinase G.


:There are two known phosphorylation-mediated inactivation mechanisms for TRPC3 channels. Protein kinase G (PKG) inactivates TRPC3 by direct phosphorylation on Thr-11 and Ser-263 of the TRPC3 proteins, and protein kinase C (PKC) inactivates TRPC3 by phosphorylation on Ser-712. In the present study, we explored the relationship between these two inactivation mechanisms of TRPC3. HEK cells were first stably transfected with a PKG-expressing construct and then transiently transfected with a TRPC3-expressing construct. Addition of 1-oleoyl-2-acetyl-sn-glycerol (OAG), a membrane-permeant analog of diacylglycerol (DAG), elicited a TRPC3-mediated [Ca2+]i rise in these cells. This OAG-induced rise in [Ca2+]i could be inhibited by phorbol 12-myristate 13-acetate (PMA), an agonist for PKC, in a dose-dependent manner. Importantly, point mutations at two PKG phosphorylation sites (T11A-S263Q) of TRPC3 markedly reduced the PMA inhibition. Furthermore, inhibition of PKG activity by KT5823 (1 microM) or H8 (10 microM) greatly reduced the PMA inhibition of TRPC3. These data strongly suggest that the inhibitory action of PKC on TRPC3 is partly mediated through PKG in these PKG-overexpressing cells. The importance of this scheme was also tested in vascular endothelial cells, in which PKG plays a pivotal functional role. In these cells, OAG-induced [Ca2+]i rise was inhibited by PMA, which activates PKC, and by 8-BrcGMP and S-nitroso-N-acetylpenicillamine (SNAP), both of which activate PKG. Importantly, the PMA inhibition on OAG-induced [Ca2+]i rise was significantly reduced by PKG inhibitor KT5823 (1 microM) or DT-3 (500 nM), suggesting an important role of PKG in the PMA-induced inhibition of TRPC channels in native endothelial cells.


J Cell Physiol


Kwan HY,Huang Y,Yao X





Has Abstract


2006-05-01 00:00:00












  • Induction of angiogenesis by smooth muscle cell-derived factor: possible role in neovascularization in atherosclerotic plaque.

    abstract::The development of atherosclerotic plaque is associated with neovascularization in the thickened intima and media of vascular walls. Neovascularization may have a role in the progression of atherosclerotic plaque as well as in the development of intraplaque hemorrhage. However, the mechanism and stimulus for neovascul...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Kuzuya M,Satake S,Esaki T,Yamada K,Hayashi T,Naito M,Asai K,Iguchi A

    更新日期:1995-09-01 00:00:00

  • Perlecan Heparan Sulfate Is Required for the Inhibition of Smooth Muscle Cell Proliferation by All-trans-Retinoic Acid.

    abstract::Smooth muscle cell (SMC) proliferation is a key process in stabilization of atherosclerotic plaques, and during restenosis after interventions. A clearer understanding of SMC growth regulation is therefore needed to design specific anti-proliferative therapies. Retinoic acid has been shown to inhibit proliferation of ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tran-Lundmark K,Tannenberg P,Rauch BH,Ekstrand J,Tran PK,Hedin U,Kinsella MG

    更新日期:2015-02-01 00:00:00

  • Assembly of the peripheral domain of the bovine vacuolar H(+)-adenosine triphosphatase.

    abstract::The biosynthesis and assembly of the peripheral sector (V1) of the vacuolar proton-translocating adenosine triphosphatase (V-ATPase) was studied in a bovine kidney epithelial cell line. Monolayer cultures of cells were metabolically radiolabeled with Tran35S-label and the V-ATPase subsequently immunoprecipitated using...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Myers M,Forgac M

    更新日期:1993-07-01 00:00:00

  • Emerging role of SIRT3 in endothelial metabolism, angiogenesis, and cardiovascular disease.

    abstract::Sirtuin 3 (SIRT3) a mitochondrial enzyme that plays an important role in energy homeostasis, cardiac remodeling, and heart failure (HF). The expression of SIRT3 declines with advanced age, cardiovascular, and metabolic diseases. Accumulating evidence suggests that SIRT3 plays a critical role in protecting the heart fr...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: He X,Zeng H,Chen JX

    更新日期:2019-03-01 00:00:00

  • Methylation-independent CHFR expression is a potential biomarker affecting prognosis in acute myeloid leukemia.

    abstract::CHFR acts as a tumor suppressor gene, which is frequently inactivated caused by its promoter hypermethylation in various solid tumors. Although a recent study showed that CHFR hypermethylation was a frequent event in acute myeloid leukemia (AML) and correlated with adverse clinical outcome, herein, we found that CHFR ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Zhou JD,Zhang TJ,Li XX,Ma JC,Guo H,Wen XM,Yao DM,Zhang W,Lin J,Qian J

    更新日期:2018-06-01 00:00:00

  • Increased P-glycoprotein messenger RNA stability in rat liver tumors in vivo.

    abstract::P-glycoproteins (Pgp) are comprised of a small family of plasma membrane proteins whose abundance in cultured cells is often associated with the multidrug resistance phenotype. Overexpression of Pgp has been observed in many types of human cancers, but the molecular basis for this overexpression has not been establish...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee CH,Bradley G,Ling V

    更新日期:1998-10-01 00:00:00

  • Evaluation of BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients.

    abstract::BAG3 is a member of human BAG (Bcl-2-associated athanogene) proteins and plays a role in apoptosis, cell adhesion, cytoskeleton remodeling, and autophagy. The aim of this study was to evaluate BAG3 levels in healthy subjects, hypertensive patients, and hypertensive diabetic patients. We enrolled 209 Caucasian adults, ...

    journal_title:Journal of cellular physiology

    pub_type: 临床试验,杂志文章


    authors: Derosa G,Maffioli P,Rosati A,M M,Basile A,D'Angelo A,Romano D,Sahebkar A,Falco A,Turco MC

    更新日期:2018-03-01 00:00:00

  • Postnatal development of peroxisomal and mitochondrial enzymes in rat liver.

    abstract::Subcellular organellles from livers of rats three days prenatal to 50 weeks postnatal were separated on sucrose gradients. The peroxisomes had a constant density of 1.243 g/ml throughout the life of the animal. The density of the mitochondria changed from about 1.236 g/ml at birth to a constant value of 1.200 g/ml aft...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Krahling JB,Gee R,Gauger JA,Tolbert NE

    更新日期:1979-12-01 00:00:00

  • Effects of macrophages and CXCR2 on adipogenic differentiation of bone marrow mesenchymal stem cells.

    abstract::Macrophages and many chemokines are closely associated with the adipogenic differentiation of bone marrow mesenchymal stem cells (MSCs), but their roles in adipogenesis and the underlying mechanisms are not fully understood. Here, we first investigated the influence of macrophages on the differentiation of MSCs in vit...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Cao D,Ma F,Ouyang S,Liu Z,Li Y,Wu J

    更新日期:2019-06-01 00:00:00

  • Therapeutics for advanced hepatocellular carcinoma: Recent advances, current dilemma, and future directions.

    abstract::Hepatocellular carcinoma (HCC) is one of the most common malignancies and is a serious threat to people's health worldwide. The prognosis of advanced HCC is dim if left untreated. In the clinic, the treatment options for advanced HCC include surgery, radiotherapy, transcatheter arterial chemoembolization, and so forth...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Zhu J,Yin T,Xu Y,Lu XJ

    更新日期:2019-08-01 00:00:00

  • Isoprenylated proteins in cultured cells: subcellular distribution and changes related to altered morphology and growth arrest induced by mevalonate deprivation.

    abstract::In the presence of lovastatin (mevinolin), an inhibitor of endogenous mevalonate synthesis, C1300 murine neuroblastoma cells incorporated (2-14C)mevalonate into several discrete polypeptides that were separable by SDS-PAGE. The electrophoretic pattern of the labeled proteins did not vary substantially when cells were ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Maltese WA,Sheridan KM

    更新日期:1987-12-01 00:00:00

  • Effect of oxygen and endotoxin on lactate dehydrogenase release, 5-hydroxytryptamine uptake, and antioxidant enzyme activities in endothelial cells.

    abstract::We compared the effects of 95% O2 (hyperoxia) alone, endotoxin (20 ng/ml) alone, and 95% O2 plus endotoxin on the release of lactate dehydrogenase (LDH), uptake of 5-hydroxytryptamine (5-HT), and antioxidant enzyme activities in porcine pulmonary arterial and aortic endothelial cells in monolayer culture. Hyperoxia in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Block ER,Patel JM,Sheridan NP

    更新日期:1985-02-01 00:00:00

  • Establishment and characterization of a telomerase-immortalized porcine bronchial epithelial cell line.

    abstract::Primary porcine bronchial epithelial cells (PBECs) are an ideal model to study the molecular and pathogenic mechanisms of various porcine respiratory pathogens. However, the short lifespan of primary PBECs greatly limit their application. Here, we isolated and cultured primary PBECs and established immortalized PBECs ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Xie X,Gan Y,Pang M,Shao G,Zhang L,Liu B,Xu Q,Wang H,Feng Y,Yu Y,Chen R,Wu M,Zhang Z,Hua L,Xiong Q,Liu M,Feng Z

    更新日期:2018-12-01 00:00:00

  • Differential binding, biological and biochemical actions of recombinant PDGF AA, AB, and BB molecules on connective tissue cells.

    abstract::We have compared the biological and biochemical properties of recombinant PDGF AA, AB, and BB using three types of fibroblastic cells: NIH/3T3, human skin fibroblast, and fetal bovine aortic smooth muscle. PDGF binding, receptor autophosphorylation, phosphatidyl inositol hydrolysis, as well as chemotactic and mitogeni...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Grotendorst GR,Igarashi A,Larson R,Soma Y,Charette M

    更新日期:1991-11-01 00:00:00

  • Syndecans in chronic inflammatory and autoimmune diseases: Pathological insights and therapeutic opportunities.

    abstract::Syndecans (SDCs) are a family of heparan sulfate proteoglycans (HSPGs) glycoproteins ubiquitously expressed on the cell surfaces and extracellular matrix of all mammalian tissues. There are four mammalian syndecans, SDC-1 thorough 4, which play a critical role in cell adhesion, migration, proliferation, differentiatio...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Agere SA,Kim EY,Akhtar N,Ahmed S

    更新日期:2018-09-01 00:00:00

  • Epidermal growth factor receptor-dependent PI3K-activation promotes restitution of wounded human gastric epithelial monolayers.

    abstract::Restitution is a crucial event during the healing of superficial injury of the gastric mucosa involving epithelial cell sheet movement into the damaged area. We demonstrated that growth factors promote the restitution of human gastric epithelial cells. However, the intracellular signaling pathways that transmit extrac...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tétreault MP,Chailler P,Beaulieu JF,Rivard N,Ménard D

    更新日期:2008-02-01 00:00:00

  • Retinoic acid induces adult muscle cell differentiation mediated by the retinoic acid receptor-alpha.

    abstract::Retinoic acid (RA) is known to induce differentiation in many cell systems. This induction is mediated by nuclear RA receptors (RARs), which act as transactivating factors belonging to the superfamily of steroid/thyroid nuclear receptors. In this study, effects of RA were studied in a mouse myogenic C2 cell line and i...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Halevy O,Lerman O

    更新日期:1993-03-01 00:00:00

  • Cytoskeleton regulates expression of genes for transforming growth factor-beta 1 and extracellular matrix proteins in dermal fibroblasts.

    abstract::Cytoskeleton not only controls cell morphology but also regulates cell growth, migration, differentiation, and gene expression, events which are fundamental to embryogenesis, carcinogenesis, and wound healing. We have recently reported that reorganization of cytoskeleton induces expression of mRNA for transforming gro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Varedi M,Ghahary A,Scott PG,Tredget EE

    更新日期:1997-08-01 00:00:00

  • NAD(P)H oxidase participates in the palmitate-induced superoxide production and insulin secretion by rat pancreatic islets.

    abstract::Nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase complex has been shown to be involved in the process of glucose-stimulated insulin secretion (GSIS). In this study, we examined the effect of palmitic acid on superoxide production and insulin secretion by rat pancreatic islets and the mechanism involved. R...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Graciano MF,Santos LR,Curi R,Carpinelli AR

    更新日期:2011-04-01 00:00:00

  • Flavonoid genistein protects bone marrow sinusoidal blood vessels from damage by methotrexate therapy in rats.

    abstract::Cancer chemotherapy can cause significant damage to the bone marrow (BM) microvascular (sinusoidal) system. Investigations must now address whether and how BM sinusoidal endothelial cells (SECs) can be protected during chemotherapy. Herein we examined the potential protective effects of genistein, a soy-derived flavon...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Hassanshahi M,Su YW,Khabbazi S,Fan CM,Chen KM,Wang JF,Qian A,Howe PR,Yan DW,Zhou HD,Xian CJ

    更新日期:2019-07-01 00:00:00

  • Inflammatory cytokines in bladder cancer.

    abstract::The presence of inflammatory cells and their products in the tumor microenvironment plays a crucial role in the pathogenesis of a tumor. Releasing the cytokines from a host in response to infection and inflammation can inhibit tumor growth and progression. However, tumor cells can also respond to the host cytokines wi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审


    authors: Shadpour P,Zamani M,Aghaalikhani N,Rashtchizadeh N

    更新日期:2019-02-18 00:00:00

  • High resolution analysis of cell cycle-correlated vimentin expression in asynchronously grown, TPA-treated MPC-11 cells by the novel flow cytometric multiparameter BrdU-Hoechst/PI and immunolabeling technique.

    abstract::High resolution, multiparameter analysis using the flow cytometric BrdU/Hoechst quenching technique has been applied to study cell cycle kinetics and vimentin expression in individual cells of asynchronously grown MPC-11 mouse plasmacytoma cell cultures treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Giese G,Kubbies M,Traub P

    更新日期:1994-11-01 00:00:00

  • Effects of interaction between estradiol-17 beta and progesterone on the proliferation of cloned breast tumor cells (MCF-7 and T47D).

    abstract::We have previously reported that the proliferation of cloned MCF-7 and T47D human mammary tumor cells can be inhibited by increasing concentrations of charcoal-dextran stripped female human serum (CDFHS). The maximal proliferation rate was restored by the addition of 3 X 10(-11) M estradiol-17 beta to the culture medi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Schatz RW,Soto AM,Sonnenschein C

    更新日期:1985-09-01 00:00:00

  • Cabbage-derived three-dimensional cellulose scaffold-induced osteogenic differentiation of stem cells.

    abstract::Herbal-derived three-dimensional scaffolds have a unique structure that represents the natural cellular microenvironment and can be potentially used for tissue engineering applications. In the present study, cabbage (Cb) leaves were decellularized and then their characteristics, such as surface roughness, wettability,...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Salehi A,Mobarhan MA,Mohammadi J,Shahsavarani H,Shokrgozar MA,Alipour A

    更新日期:2020-12-30 00:00:00

  • Protein kinases and adherens junction dynamics in the seminiferous epithelium of the rat testis.

    abstract::Earlier studies in multiple epithelia have shown that cell-cell actin-based adherens junction (AJ) dynamics are regulated, at least in part, by the interplay of kinases and phosphatases that determines the intracellular phosphoprotein content. Yet it is virtually unknown regarding the role of protein kinases in Sertol...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Lee NP,Cheng CY

    更新日期:2005-02-01 00:00:00

  • γ-d-Glutamyl-meso-diaminopimelic acid induces autophagy in bovine hepatocytes during nucleotide-binding oligomerization domain 1-mediated inflammation.

    abstract::Autophagy is a crucial cellular homeostatic process and an important part of the host defense system. Dysfunction in autophagy enhances tissue susceptibility to infection and multiple diseases. However, the role of nucleotide oligomerization domain 1 (NOD1) in autophagy in bovine hepatocytes is not well known. Therefo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Roy AC,Chang G,Roy S,Ma N,Gao Q,Shen X

    更新日期:2020-12-24 00:00:00

  • Normal skin wound and hypertrophic scar myofibroblasts have differential responses to apoptotic inductors.

    abstract::During wound healing, myofibroblasts play a central role in matrix formation and wound contraction. At the end of healing, there is evidence that myofibroblasts disappear via apoptotic pathways. Hypertrophic scars are a fibroproliferative disorder that leads to considerable morbidity. It has been postulated that a def...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Moulin V,Larochelle S,Langlois C,Thibault I,Lopez-Vallé CA,Roy M

    更新日期:2004-03-01 00:00:00

  • Alternative splicing of CARMA2/CARD14 transcripts generates protein variants with differential effect on NF-κB activation and endoplasmic reticulum stress-induced cell death.

    abstract::The caspase recruitment domain (CARD)-containing proteins CARMA1-3 share high degree of sequence, structure and functional homology. Whereas CARMA1 and CARMA3 have been identified as crucial components of signal transduction pathways that lead to activation of NF-κB transcription factor, little is known about the func...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Scudiero I,Zotti T,Ferravante A,Vessichelli M,Vito P,Stilo R

    更新日期:2011-12-01 00:00:00

  • Phosphoinositide 3-kinase/Akt involvement in arsenic trioxide resistance of human leukemia cells.

    abstract::The purpose of this study was to evaluate the possible involvement of the phosphoinositide 3-kinase (PI3K)/Akt survival pathway in determining resistance to arsenic trioxide (As2O3)-induced apoptosis. We employed a HL60 cell clone (HL60AR) with a constitutively active PI3K/Akt survival pathway, as well as U937 and K56...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Tabellini G,Cappellini A,Tazzari PL,Falà F,Billi AM,Manzoli L,Cocco L,Martelli AM

    更新日期:2005-02-01 00:00:00

  • Studies on the mechanism of Ca2+ stimulation of plasminogen activator synthesis/release by Swiss 3T3 cells.

    abstract::Stimulation of postconfluent Swiss 3T3 cells in serum-free medium with 4.3 mM Ca2+ results in marked increases in both released and cell-associated plasminogen activator (PA). Increased release of PA commenced approximately 10 to 12 hours post-stimulation and continued to increase steadily until 48 hours at which time...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章


    authors: Chou IN,Cox R,Black PH

    更新日期:1979-09-01 00:00:00