Glucosamine promotes osteogenic differentiation of dental pulp stem cells through modulating the level of the transforming growth factor-beta type I receptor.

Abstract:

:Dental pulp stem cells (DPSCs) are clonogenic, self-renewing, and multi-potential DPSCs capable of differentiating into osteoblasts. In this study, primary cell cultures were obtained from human dental pulp tissue of developing third molars, and flow cytometry was used to sort the subpopulation of DPSCs with STRO-1 and CD146 double-positive expression (denoted "DPSCs"). It was noted that DPSCs exhibited superior clonogenic potential and osteogenic differentiation capability than the dental pulp cell subpopulation with STRO-1 and CD146 double-negative expression (denoted DPCs). Furthermore, a low concentration (0.005 mg/ml) of exogenous glucosamine (GlcN) was effective in promoting the early osteogenic differentiation of DPSCs through the transforming growth factor-beta receptor (TGF-betar) type I and Smads signal pathways, which upregulated the Runt-related transcription factor 2/core-binding factor alpha1 (Runx2/Cbfa1) and alkaline phosphatase at both the mRNA and protein levels. In the presence of osteogenic supplements, GlcN-treated DPSCs produced more mineralized-matrix deposition than did the untreated groups. Taken together, this study demonstrates the capacity of GlcN to promote the osteogenic differentiation of human DPSCs, and the underlying mechanism involves a TGF-betar-dependent Smad signal pathway.

journal_name

J Cell Physiol

authors

Huang CH,Tseng WY,Yao CC,Jeng JH,Young TH,Chen YJ

doi

10.1002/jcp.22206

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

140-51

issue

1

eissn

0021-9541

issn

1097-4652

journal_volume

225

pub_type

杂志文章
  • Chrysophanol Induces Apoptosis of Choriocarcinoma Through Regulation of ROS and the AKT and ERK1/2 Pathways.

    abstract::Chrysophanol is an anthraquinone compound, mainly isolated from rhubarb, with anti-cancer effects on some types of cancer cells. However, effects of chrysophanol on human choriocarcinoma cells are not known. Therefore, the objective of this study was to determine effects of chrysophanol on choriocarcinoma cells (JAR a...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25423

    authors: Lim W,Yang C,Bazer FW,Song G

    更新日期:2017-02-01 00:00:00

  • N-carbamoyloxyurea-resistant Chinese hamster ovary cells with elevated levels of ribonucleotide reductase activity.

    abstract::We describe the isolation and characterization of a Chinese hamster ovary cell line selected for resistance to N-carbamoyloxyurea. Using the mammalian cell permeabilization assay developed in our laboratory, a detailed analysis of the target enzyme, ribonucleotide reductase (EC 1.17.4.1), was carried out. Both drug-re...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041060218

    authors: Hards RG,Wright JA

    更新日期:1981-02-01 00:00:00

  • Regulation of Cu,Zn-superoxide dismutase in bovine pulmonary artery endothelial cells.

    abstract::To evaluate the regulation of endothelial cell Cu,Zn-SOD, we have exposed bovine pulmonary artery endothelial cells in culture to hyperoxia and hypoxia, second messengers or related agonists, hormones, free radical generating systems, endotoxin, and cytokines and have measured Cu,Zn-SOD protein of these cells by an EL...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041530308

    authors: Kong XJ,Fanburg BL

    更新日期:1992-12-01 00:00:00

  • Susceptibility to verotoxin as a function of the cell cycle.

    abstract::Infection with Verotoxin producing Escherichia coli (VTEC) has been implicated in hemolytic uremic syndrome, the leading cause of pediatric renal failure. Verotoxin (VT) binds to globotriaosylceramide (Gal alpha 1-4Gal beta 1-4GlcCer Gb3) in susceptible cells. Gb3 is required for cytotoxicity and toxin-resistant cells...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041500324

    authors: Pudymaitis A,Lingwood CA

    更新日期:1992-03-01 00:00:00

  • Early replication and the apoptotic pathway.

    abstract::In higher eukaryotes there is a link between time of replication and transcription. It is generally accepted that genes that are actively transcribed are replicated in the first half of S phase while inactive genes replicate in the second half of S phase. We have recently reported that in normal human fibroblasts ther...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.21156

    authors: Cohen SM,Cordeiro-Stone M,Kaufman DG

    更新日期:2007-11-01 00:00:00

  • Consequences of aberrant ornithine decarboxylase regulation in rat hepatoma cells.

    abstract::DH23A cells, an alpha-difluoromethylornithine (DFMO)-resistant variant of rat hepatoma tissue culture cells (HTC), contain high levels of very stable ornithine decarboxylase (ODC). In the absence of DFMO, the high ODC activity results in a large accumulation of endogenous putrescine. Concomitant with the putrescine in...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041580205

    authors: Tome ME,Fiser SM,Gerner EW

    更新日期:1994-02-01 00:00:00

  • Acute ethanol exposure-induced autophagy-mediated cardiac injury via activation of the ROS-JNK-Bcl-2 pathway.

    abstract::Binge drinking is associated with increased cardiac autophagy, and often triggers heart injury. Given the essential role of autophagy in various cardiac diseases, this study was designed to investigate the role of autophagy in ethanol-induced cardiac injury and the underlying mechanism. Our study showed that ethanol e...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25934

    authors: Zhu Z,Huang Y,Lv L,Tao Y,Shao M,Zhao C,Xue M,Sun J,Niu C,Wang Y,Kim S,Cong W,Mao W,Jin L

    更新日期:2018-02-01 00:00:00

  • Molecular cloning of Drosophila HCF reveals proteolytic processing and self-association of the encoded protein.

    abstract::HCF-1 functions as a coactivator for herpes simplex virus VP16 and a number of mammalian transcription factors. Mature HCF-1 is composed of two subunits generated by proteolytic cleavage of a larger precursor at six centrally-located HCF(PRO) repeats. The resulting N- and C-terminal subunits remain tightly associated ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.10193

    authors: Mahajan SS,Johnson KM,Wilson AC

    更新日期:2003-02-01 00:00:00

  • Mitogenic effect of erythropoietin on neonatal rat cardiomyocytes: signal transduction pathways.

    abstract::The mitogenic effect of recombinant human erythropoietin (rHuEpo) on primary cultures of neonatal rat cardiac myocytes was observed. rHuEpo triggered a dose-dependent increase in myocyte proliferation. The hormone effect over optimally grown control culture 1 day after addition was maximum with 0.5 U/ml and was inhibi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199606)167:3<461::AID-JCP1

    authors: Wald MR,Borda ES,Sterin-Borda L

    更新日期:1996-06-01 00:00:00

  • Role of phospholipase C and D signalling pathways in vasopressin-dependent myogenic differentiation.

    abstract::Arg8-vasopressin (AVP) is a potent inducer of myogenic differentiation stimulating the expression of myogenic regulatory factors. To understand the mechanism of its effect on myogenesis, we investigated the early signals induced by AVP in myogenic target cells. In the rat skeletal muscle cell line L6, AVP selectively ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199704)171:1<34::AID-JCP5>

    authors: Naro F,Donchenko V,Minotti S,Zolla L,Molinaro M,Adamo S

    更新日期:1997-04-01 00:00:00

  • Effects of retinoic acid on protein synthesis in cultured melanoma cells.

    abstract::Retinoic acid reduces the growth rate of mouse S91 melanoma cells in culture and increases the proportion of cells in the G1 phase of the cell cycle. Because of the integral role protein synthesis has been shown to play in growth control we studied the effect of retinoic acid on the protein synthesis machinery with a ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041130110

    authors: Lotan R,Fischer I,Meromsky L,Moldave K

    更新日期:1982-10-01 00:00:00

  • An Innovative Assay for the Analysis of In Vitro Endothelial Remodeling: Experimental and Computational Evidence.

    abstract::The molecular and cellular mechanisms underlying vascular remodeling are currently investigated by experimental strategies which aim to mimic the complex environmental conditions found in vivo. Some of them focus on the tubulogenic activity of dispersed endothelial cell populations, while others evaluate vascular spro...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25468

    authors: Scianna M,Bassino E,Munaron L

    更新日期:2017-02-01 00:00:00

  • Regulatory role of microRNA-320a in the proliferation, migration, invasion, and apoptosis of trophoblasts and endothelial cells by targeting estrogen-related receptor γ.

    abstract::Recently, microRNAs (miRNAs) have been demonstrated to participate in many physiological and biological processes, especially by acting as circulating biomarkers or modulators in cell differentiation. Therefore, the aim of the current study was to clarify whether microRNA-320a (miR-320a) regulates the proliferation, m...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.26842

    authors: Liu RH,Meng Q,Shi YP,Xu HS

    更新日期:2018-01-01 00:00:00

  • LIGHT regulates CD86 expression on dendritic cells through NF-kappaB, but not JNK/AP-1 signal transduction pathway.

    abstract::The members of the tumor necrosis factor (TNF) family play pivotal roles in the regulation of the immune system. LIGHT is a type II transmembrane protein belonging to the TNF family that was originally identified as a weak inducer of apoptosis. This cytokine has been extensively studied for its role in T cell regulati...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20420

    authors: Zou GM,Hu WY

    更新日期:2005-12-01 00:00:00

  • Interference with cellular differentiation by D-serine through antagonism at N-methyl-D-aspartate receptors composed of NR1 and NR3A subunits in chondrocytes.

    abstract::Serine racemase (SR) is responsible for the biosynthesis of D-serine (D-Ser), an endogenous co-agonist for the glycine (Gly)-binding site on N-methyl-D-aspartate (NMDA) receptors, from L-Ser in the brain. We have previously demonstrated high expression of SR by chondrocytes in cartilage. In this study, we attempted to...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21821

    authors: Takarada T,Takahata Y,Iemata M,Hinoi E,Uno K,Hirai T,Yamamoto T,Yoneda Y

    更新日期:2009-09-01 00:00:00

  • Epac/Rap and PKA are novel mechanisms of ANP-induced Rac-mediated pulmonary endothelial barrier protection.

    abstract::Acute lung injury, sepsis, lung inflammation, and ventilator-induced lung injury are life-threatening conditions associated with lung vascular barrier dysfunction, which may lead to pulmonary edema. Increased levels of atrial natriuretic peptide (ANP) in lung circulation reported in these pathologies suggest its poten...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21354

    authors: Birukova AA,Zagranichnaya T,Alekseeva E,Bokoch GM,Birukov KG

    更新日期:2008-06-01 00:00:00

  • Kinin B1 Receptor Deletion Affects Bone Healing in Type 1 Diabetic Mice.

    abstract::The effects of kinin B1 receptor (B1 R) deletion were examined on femur bone regeneration in streptozotocin (STZ)-type 1 diabetes. Diabetes induction in wild-type C57/BL6 (WTC57BL6) mice led to decrease in body weight and hyperglycemia, compared to the non-diabetic group of the same strain. The lack of B1 R did not af...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.25034

    authors: Cignachi NP,Pesquero JB,Oliveira RB,Etges A,Campos MM

    更新日期:2015-12-01 00:00:00

  • BDNF regulates GLAST and glutamine synthetase in mouse retinal Müller cells.

    abstract::This study investigated whether brain-derived neurotrophic factor (BDNF) regulates the L-glutamate/L-aspartate transporter (GLAST) and glutamine synthetase (GS) in mouse retinal Müller cells (RMCs) under normal and hypoxic conditions. Mouse RMCs were treated with recombinant human BDNF (50, 75, 100, 125, or 150  ng/ml...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.22762

    authors: Dai M,Xia XB,Xiong SQ

    更新日期:2012-02-01 00:00:00

  • Ubiquitin-specific protease 14 is a new therapeutic target for the treatment of diseases.

    abstract::Ubiquitin-specific protease 14 (USP14) is a ubiquitin-specific protease that is associated with the proteasome and plays important roles in cellular functions, viral infection, inflammatory responses, neurodegenerative diseases, and tumorigenesis. USP14 appears to have a dual function in regulating intracellular prote...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.30124

    authors: Wang D,Ma H,Zhao Y,Zhao J

    更新日期:2020-11-01 00:00:00

  • Effect of aging on the mechanisms of PTH-induced calcium influx in rat intestinal cells.

    abstract::We have investigated the effects of aging on parathyroid hormone (PTH) modulation of intracellular calcium homeostasis and their relationship to signal transduction pathways in isolated rat duodenal cells (enterocytes). PTH (10(-8)-10(-9) M) increased enterocyte (45)Ca(2+) influx and intracellular Ca(2+) concentration...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(200003)182:3<429::AID-JCP1

    authors: Massheimer V,Picotto G,Boland R,De Boland AR

    更新日期:2000-03-01 00:00:00

  • HER2 signaling enhances 5'UTR-mediated translation of c-Myc mRNA.

    abstract::The increased levels of c-Myc protein observed previously in an ovarian carcinoma cell line stably transfected to express HER2 has suggested a role for the HER2 pathway in c-Myc expression. Analysis of HER2-transfected cells stimulated with heregulin beta1 (HRG) revealed increased c-Myc protein levels but not a corres...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.20012

    authors: Galmozzi E,Casalini P,Iorio MV,Casati B,Olgiati C,Ménard S

    更新日期:2004-07-01 00:00:00

  • Evidence for the participation of nerve growth factor and its low-affinity receptor (p75NTR) in the regulation of the myogenic program.

    abstract::We have studied expression and function of neurotrophins and their receptors during myogenic differentiation of C2C12 cells, a clonal cell line derived from mouse muscle that is capable of in vitro differentiation. The genes coding for nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and their commo...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199807)176:1<10::AID-JCP2>

    authors: Seidl K,Erck C,Buchberger A

    更新日期:1998-07-01 00:00:00

  • Induction of Na+ channel voltage sensitivity in Xenopus oocytes depends on Ca2+ mobilization.

    abstract::An unusual inward current which is slowly elicited in the Xenopus oocyte membrane during sustained depolarization is reportedly carried by Na+. It is thought that Na+ selective channels are in some way induced to become voltage-sensitive by the depolarization. Earlier studies report that the induction process involves...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4652(199902)178:2<258::AID-JCP1

    authors: Charpentier G,Kado RT

    更新日期:1999-02-01 00:00:00

  • A new insight into thymosin β4, a promising therapeutic approach for neurodegenerative disorders.

    abstract::Thymosin β4 (Tβ4), a G-actin-sequestering secreted peptide, improves neurovascular remodeling and central nervous system plasticity, which leads to neurological recovery in many neurological diseases. Inflammatory response adjustment and tissue inflammation consequences from neurological injury are vital for neurologi...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.29293

    authors: Shomali N,Baradaran B,Deljavanghodrati M,Akbari M,Hemmatzadeh M,Mohammadi H,Jang Y,Xu H,Sandoghchian Shotorbani S

    更新日期:2020-04-01 00:00:00

  • Radiosensitivity enhancement by Co-NMS-mediated mitochondrial impairment in glioblastoma.

    abstract::We investigated the radiosensitizing effects of Co-NMS, a derivative of nimesulide based on a cobalt carbonyl complex, on malignant glioma cells. In the zebrafish exposed to Co-NMS ranging from 5 to 20 μM, cell death and heat shock protein 70 expression in the brain and neurobehavioral performance were evaluated. Our ...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29774

    authors: Liu Y,Zhang T,Li G,Li S,Li J,Zhao Q,Wu Q,Xu D,Hu X,Zhang L,Li Q,Zhang H,Liu B

    更新日期:2020-12-01 00:00:00

  • p38SJ, a novel DINGG protein protects neuronal cells from alcohol induced injury and death.

    abstract::Ethanol induces neuronal cell injury and death by dysregulating several signaling events that are controlled, in part, by activation of MAPK/ERK1/2 and/or inactivation of its corresponding phosphatase, PP1. Recently, we have purified a novel protein of 38 kDa in size, p38SJ, from a callus culture of Hypericum perforat...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.21903

    authors: Amini S,Merabova N,Khalili K,Darbinian N

    更新日期:2009-12-01 00:00:00

  • A role for soluble cAMP phosphodiesterases in differentiation of 3T3-L1 adipocytes.

    abstract::Differentiation of 3T3-L1 adipocytes, monitored by accumulation of neutral lipid and by increase in alpha-glycerophosphate dehydrogenase activity, is accelerated by incubation of confluent 3T3-L1 fibroblasts in media containing insulin, dexamethasone and isobutylmethylxantine (IBMX). IBMX inhibits cyclic nucleotide ph...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.1041240204

    authors: Elks ML,Manganiello VC

    更新日期:1985-08-01 00:00:00

  • Novel insights into Dhh signaling in antler chondrocyte proliferation and differentiation: Involvement of Foxa.

    abstract::The desert hedgehog (Dhh) is crucial for spermatogenesis and Leydig cell differentiation, but little is known regarding its physiological function in cartilage. In this study, Dhh mRNA was abundant in antler chondrocytes, where it advanced cell proliferation concomitant with accelerated transition from the G1 to the S...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.29528

    authors: Ma L,Duan CC,Yang ZQ,Ding JL,Liu S,Yue ZP,Guo B

    更新日期:2020-09-01 00:00:00

  • mTOR and Beclin1: Two key autophagy-related molecules and their roles in myocardial ischemia/reperfusion injury.

    abstract::Autophagy is the general term of lysosomal degradation of substances in cells, which is considered the key to maintaining the normal structure and function of the heart. It also has a correlation with several heart diseases, in particular, myocardial ischemia/reperfusion (I/R) injury. At the stage of myocardial ischem...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章,评审

    doi:10.1002/jcp.28125

    authors: Shi B,Ma M,Zheng Y,Pan Y,Lin X

    更新日期:2019-08-01 00:00:00

  • C/EBPα and the Vitamin D Receptor Cooperate in the Regulation of Cathelicidin in Lung Epithelial Cells.

    abstract::1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) and the vitamin D receptor (VDR) have been reported to have an important role in the regulation of innate immunity. We earlier reported that the antimicrobial peptide cathelicidin is induced by 1,25(OH)2D3 in normal human bronchial epithelial cells with a resultant increase in an...

    journal_title:Journal of cellular physiology

    pub_type: 杂志文章

    doi:10.1002/jcp.24729

    authors: Dhawan P,Wei R,Sun C,Gombart AF,Koeffler HP,Diamond G,Christakos S

    更新日期:2015-02-01 00:00:00