Abstract:
:The molecular and cellular mechanisms underlying vascular remodeling are currently investigated by experimental strategies which aim to mimic the complex environmental conditions found in vivo. Some of them focus on the tubulogenic activity of dispersed endothelial cell populations, while others evaluate vascular sprouting. Here we propose a new method to assess matrigel invasion starting from confluent or subconfluent monolayers of human microvascular ECs (HMVEC) seeded on different substrates. The experimental setting is also validated by an improved hybrid multiscale mathematical approach, which integrates a mesoscopic grid-based cellular Potts model, that describes HMVEC phenomenology, with a continuous one, accounting for the kinetics of diffusing growth factors. Both experimental and theoretical approaches show that the endothelial potential to invade, migrate, and organize in tubule structures is a function of selected environmental parameters. The present methodology is intended to be simple to use, standardized for rapid screening and suitable for mechanistic studies. J. Cell. Physiol. 232: 243-248, 2017. © 2016 Wiley Periodicals, Inc.
journal_name
J Cell Physioljournal_title
Journal of cellular physiologyauthors
Scianna M,Bassino E,Munaron Ldoi
10.1002/jcp.25468subject
Has Abstractpub_date
2017-02-01 00:00:00pages
243-248issue
2eissn
0021-9541issn
1097-4652journal_volume
232pub_type
杂志文章abstract::Staurosporine, a microbial-derived protein kinase inhibitor, reversibly blocked non-synchronized, replicating cultures of the human lung epithelial cell line EKVX in the G1 phase of cell cycle and inhibited DNA synthesis and cell replication. The mechanism of this cell-cycle arrest in EKVX cells by staurosporine was l...
journal_title:Journal of cellular physiology
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更新日期:1987-02-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章,评审
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更新日期:2000-09-01 00:00:00
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journal_title:Journal of cellular physiology
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更新日期:2017-07-01 00:00:00
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更新日期:2000-04-01 00:00:00
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journal_title:Journal of cellular physiology
pub_type: 杂志文章
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更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Journal of cellular physiology
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doi:10.1002/jcp.27360
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:Surgical stress has been suggested to facilitate colon cancer growth and metastasis. However, the precise mechanisms by which surgical trauma promotes colon cancer progression remain poorly understood. METHODS:To unravel the mechanisms underlying surgery-induced colon cancer progression, a syngenic transpla...
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更新日期:2019-04-01 00:00:00
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更新日期:1977-02-01 00:00:00
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更新日期:2007-12-01 00:00:00